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Comparative histomorphometric study of intraepithelial papillary capillaries on leukoplakia with/without different dysplastic grading and squamous cell carcinoma of the oral mucosa

  • Original Article
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Oral Cancer

Abstract

Purpose

Tumor growth is closely related to the development of an intrinsic vascular network. However, microvascular appearance was not regarding highly to pathological diagnosis, though clinical applicability of endoscopic narrow-bad imaging (NBI) is developed in the oral cavity. To analyze changes of intraepithelial papillary capillary loop (IPCLs) patterns as the utility for pathological diagnosis, comparative histomorphometric analysis of IPCLs among squamous cell hyperplasia, leukoplakia with low- or high-grade dysplasia, and oral squamous cell carcinoma (OSCC) had performed.

Methods

Subjects were 40 clinically hyperkeratosis, histologically consisted 5 groups; each 10 of squamous cell hyperplasia, low- or high-grade dysplasia, OSCC and normal mucosae. Sections with smooth muscle actin and CD34 immunoreactivities were used to examine comparative histomorphometric analysis on the basis of IPCL irregularities. The area, shortest diameter, proximity to the basement membrane and circularity were measured using Win Roof image analysis software. Statistical analyses were done by Kruskal–Wallis and Steel–Dwass tests. Values of p < 0.05 were considered significant.

Results

Larger vessel diameter/areas, more extension and smaller circularity with dysplastic grading, and OSCC showed the most prominent of them. In short, the changes in vessels of IPCLs in high-grade dysplasia and OSCC involve two factors: increased vessel diameter and prolongation of IPCLs toward the surface.

Conclusions

The microvascular irregularity and proliferation composed by IPCLs and IPCL like abnormal capillaries were observed pathologically in squamous epithelial lesions of the oral cavity. The change of IPCLs patterns can be of help in detecting for high-grade dysplasia or OSCC.

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Authors and Affiliations

  1. Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

    Kazuhiro Hasegawa, Masahiro Higuchi, Masaaki Suemitsu, Miyuki Morikawa, Tadahiko Utsunomiya & Kayo Kuyama

  2. Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

    Hiroyuki Sakamaki, Ko Ito & Toshirou Kondoh

  3. Department of Dental Hygiene, Faculty of Health Care Sciences, Chiba Prefectural University of Health Sciences, Chiba, Japan

    Hiroyuki Sakamaki

  4. Department of Preventive and Public Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

    Chieko Taguchi

Authors
  1. Kazuhiro Hasegawa
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  2. Hiroyuki Sakamaki
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  3. Masahiro Higuchi
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  4. Masaaki Suemitsu
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  5. Chieko Taguchi
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  6. Ko Ito
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  8. Tadahiko Utsunomiya
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  9. Toshirou Kondoh
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  10. Kayo Kuyama
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Corresponding author

Correspondence to Kayo Kuyama.

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Informed consent was obtained from all individuals included in the study.

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The authors declare that they have no conflict interest.

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All procedure performed in studies involving human participants were in accordance with the ethical standards of the Committee on Studies Involving Human Beings of Nihon University School of Dentistry at Matsudo (EC-15-14-033-1) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Hasegawa, K., Sakamaki, H., Higuchi, M. et al. Comparative histomorphometric study of intraepithelial papillary capillaries on leukoplakia with/without different dysplastic grading and squamous cell carcinoma of the oral mucosa. Oral Cancer 2, 37–44 (2018). https://doi.org/10.1007/s41548-018-0007-y

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  • DOI: https://doi.org/10.1007/s41548-018-0007-y

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