Skip to main content

Advertisement

SpringerLink
Log in

Identification of plant-parasitic nematodes associated with cut flowers

  • Original Article
  • Published:
Journal of Plant Diseases and Protection Aims and scope Submit manuscript

Abstract

Carnation, gerbera and lisianthus are an important part of the cut flower market worldwide, but plant-parasitic nematodes (PPN) affect their production and quality. Therefore, rapid and accurate identification of PPNs is required for effective pest management programmes and crop rotation. In this study, a survey was carried out in the Antalya province of Turkey, an area where carnation, gerbera, lisianthus and chrysanthemum are commercially grown. A total of 79 soil samples were collected from greenhouses in the area, and 12 species-specific primers for identification of Meloidogyne, Pratylenchus and Rotylenchulus species were used for molecular detection. Pratylenchus thornei, M. incognita, P. neglectus, M. javanica, M. arenaria and M. hapla were detected in 98.73%, 88.60%, 65.82%, 34.17%, 22.78% and 5.06% of samples, respectively. Results showed combination of different PPN species in the soil of greenhouses where cut flowers were grown. These findings may be used to improve management practices, reducing losses in cut flower production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Adam MAM, Phillips MS, Blok VC (2007) Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathol 56:190–197

    Article  CAS  Google Scholar 

  • Agudelo P, Robbins RT, Stewart JMcD, Szalanski AL (2005) Intraspecific Variability of Rotylenchulus reniformis from Cotton-growing Regions in the United States. J Nematol 37:105–114

    PubMed  PubMed Central  Google Scholar 

  • Agudelo PA, Lewis SA, Abril MA (2006) First report of root-knot nematode Meloidogyne javanica on chrysanthemum in Colombia. Plant Dis 90:828

    Article  CAS  PubMed  Google Scholar 

  • Al-Banna L, Ploeg AT, Williamson WM, Kaloshian I (2004) Discrimination of six Pratylenchus species using PCR and species specific primers. J Nematol 36:142–146

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bala G, Hosein F (1996) Plant parasitic nematodes associated with anthuriums and other tropical ornamentals. Nematropica 26:9–14

    Google Scholar 

  • Cabos RYM, Wang K-H, Sipes BS, Heller WP, Matsumoto TK (2013) Detection of plant-parasitic nematode DNA in the gut of predatory and omnivorous nematodes. Nematropica 43:44–48

    Google Scholar 

  • Deimi AM, Chitambar JJ, Maafi ZT (2008) Nematodes associated with flowering ornamental plants in Mahallat, Iran. Nematol Medit 36:115–123

    Google Scholar 

  • Devran Z, Söğüt MA (2009) Distribution and identification of Root-knot nematodes from Turkey. J Nematol 41:128–133

    CAS  PubMed  PubMed Central  Google Scholar 

  • Devran Z, Polat İ, Mıstanoğlu İ, Baysal Ö (2018) A novel multiplex PCR tool for simultaneous detection of three root-knot nematodes. Australas Plant Pathol 47:1–4

    Article  Google Scholar 

  • Gleason CA, Liu QL, Williamson VM (2008) Silencing a candidate nematode effector gene corresponding to the tomato resistance gene Mi-1 leads to acquisition of virulence. Mol Plant Mıcrobe Interact 21:576–585

    Article  CAS  PubMed  Google Scholar 

  • Handoo ZA, Golden AM (1989) A key and diagnostic compendium to the species of the genus Pratylenchus Filipjev 1936 (Lesion Nematodes). J Nematol 21:202–218

    CAS  PubMed  PubMed Central  Google Scholar 

  • Harris TS, Sandal LJ, Powers TO (1990) Identification of single Meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial DNA. J Nematol 22:518–524

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hooper DJ (1986) Extraction of free-living stages from soil. In: Southey JF (ed) Laboratory methods for work with plant and soil nematodes. Her Majesty’s Stationery Office, London, pp 5–30

    Google Scholar 

  • Hye-Rim H, Jae Kook L, Dong Ro D, Man Jong H, Byeong Yong P (2006) Occurrence of plant parasitic nematodes in chrysanthemum and ITS and D3-28S rDNA characterization of Pratylenchus spp. Korean J Appl Entomol 45:293–299

    Google Scholar 

  • Jepson SB (1987) Identification of Root-knot nematodes (Meloidogyne species), 1st edn. CAB Int, Wallingford

    Google Scholar 

  • Kepenekci İ, Öztürk G (2002) Ülkemizde süs bitkilerinde sorun olan bitki paraziti nematodlar. In: II. Ulusal Süs Bitkileri Kongresi, Antalya, Türkiye, pp 208–215

  • Lamberti F, Tacconi R, Marinari A, D’Errico FP, Basile M (1987) Major plant parasitic nematodes associated with flower crops in Italy and their control. Difesa delle Piante 10:77–84

    Google Scholar 

  • Langat JK, Kimenju JW, Mutua GK, Muiru WM, Otieno W (2008) Response of free-living nematodes to treatments targeting plant parasitic nematodes in carnation. Asia J Plant Sci 7:467–472

    Article  Google Scholar 

  • Manju P, Subramanian S (2015) Survey of plant parasitic nematodes associated with gerbera in Tamil Nadu. Int J Scı Nat 6:586–589

    Google Scholar 

  • Marroquin A, Arbelaez G (1992) Chemical control of cyst nematode, Heterodera trifolii on miniature carnation. In: ISHS Acta Horticulturae 307: IV international symposium on carnation culture, pp 131–136

  • Nagesh M, Parvatha Reddy P (1996) Management of Meloidogyne incognita on carnation and gerbera in commercial polyhouses. In: Crop productivity and sustainability—shaping the future, 2nd International crop science congress. NAAS, New Delhi, India, p 349

  • Nagesh M, Parvatha Reddy P (2000) Crop loss in carnation and gerbera due to the root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood. Pest Manag Hortic Ecosyst 6:158–159

    Google Scholar 

  • Neves CG, Belle C, Nascimento MB, Grolli PR, Gomes CB, Barros DR (2017) First Report of Meloidogyne arenaria on Lisianthus (Eustoma grandiflorum) in Brazil. Plant Dis 101:511

    Article  Google Scholar 

  • Nirmal Johnson SB (2000) Studies on nematode pests of cutflowers with special reference to carnation, gerbera, gladiolus and Asiatic lily. Dissertation, Tamil Nadu Agricultural University

  • Phyllis J (1997) Outlook on plant nematodes and their control in Cyprus. http://www.fao.org/docrep/v9978e/v9978e0d.htm Accessed 19 Dec 2018

  • Powers TO, Todd TC, Burnell AM, Murray PCB, Fleming CC, Szalanski AL, Adams BA, Harris TS (1997) The internal transcribed space region as a taxonomic marker for nematodes. J Nematol 29:441–450

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sasser JN, Freckman DW (1987) A world prospective in nematology: the role of society. In: Veech JA, Dickson DW (eds) Vistas on nematology. Society of Nematologists Inc., Hyattsville, pp 7–14

    Google Scholar 

  • Sharma S, Sharma N (2008) Carnation diseases and their management—a review. Agrıc Rev 29:11–20

    Google Scholar 

  • Söğüt MA, Devran Z (2011) Distribution and molecular identification of root lesion nematodes in temperate fruit orchards of Turkey. Nematropıca 41:91–99

    Google Scholar 

  • Taşçıoğlu Y, Sayın C (2005) Cut flower production and export structure in Turkey. Medit Agric Sci 18:343–354

    Google Scholar 

  • Trademap (2016) Trade Statistics For International Business Development http://www.trademap.org/Country_SelProduct_TS.aspx Accessed 19 Dec 2018

  • Troccoli A, De Luca F, Hando ZA, Di Vito M (2008) Morphological and molecular characterization of Pratylenchus lentis n. sp. (Nematoda: Pratylenchidae) from Sicily. J Nematol 40:190–196

    CAS  PubMed  PubMed Central  Google Scholar 

  • Turkish Statistical Institute (2018) Crop Production Statistics, https://biruni.tuik.gov.tr/bitkiselapp/bitkisel.zul Accessed 26 April 2018

  • Waeyenberge L, Ryss A, Moens M, Pinochet J, Vrain T (2000) Molecular characterization of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology 2:135–142

    Article  CAS  Google Scholar 

  • Wishart J, Phillips MS, Blok VC (2002) Ribosomal Intergenic Spacer: a Polymerase chain reaction diagnostic for Meloidogyne chitwoodi, M. fallax, and M. hapla. Nematology 92:884–892

    CAS  Google Scholar 

  • Yamamoto E, Toida Y (1995a) Fauna of plant parasitic nematodes in temperate region of Japan. Jpn Int Res Center Agric Sci J 2:43–48

    Google Scholar 

  • Yamamoto E, Toida Y (1995b) Fauna of plant parasitic nematodes in temperate region of Japan-2. Survey in China Perfecture. Jpn Int Res Center Agric Sci J 2:37–42

    Google Scholar 

  • Yan G, Smiley RW, Okubara PA, Skantar A, Easley SA, Sheedy JG, Thompson AL (2008) Detection and discrimination of Pratylenchus neglectus and P. thornei in DNA extracts from soil. Plant Dis 92:1480–1487

    Article  CAS  PubMed  Google Scholar 

  • Zijlstra C, Donkers-Venne DTHM, Fargette M (2000) Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterized amplified region (SCAR) based PCR assays. Nematology 2:847–853

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, 07058, Antalya, Turkey

    Elvan Sert Çelik, Tevfik Özalp, İbrahim Mıstanoğlu & Zübeyir Devran

Authors
  1. Elvan Sert Çelik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tevfik Özalp
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. İbrahim Mıstanoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zübeyir Devran
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zübeyir Devran.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sert Çelik, E., Özalp, T., Mıstanoğlu, İ. et al. Identification of plant-parasitic nematodes associated with cut flowers. J Plant Dis Prot 126, 409–420 (2019). https://doi.org/10.1007/s41348-019-00235-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s41348-019-00235-7

Keywords

Search

Navigation