Abstract
This study was conducted to evaluate the suppression of Pyricularia oryzae, Colletotrichum coccodes, Rhizoctonia solani, Phytophthora capsici, and Botrytis cinerea, as well as the acaricidal activities against the two-spotted spider mite (Tetranychus urticae) and the weed control efficacy of solvent fractions of Pheum palmatum extract. In addition, we determined the inhibitory activities of the main bioactive substances of R. palmatum (aloe-emodin, emodin, chrysophanol, and physcion) against the aforementioned pathogens. The suppression rates of 5% ethylacetate and BuOH fractions against P. oryzae were 70 and 52%, respectively. However, the other fractions including hexane showed very little suppression of P. oryzae. Moreover, C. coccodes, B. cinerea, R. solani, and P. capsici were suppressed by 61, 47, 58, and 91% in response to treatment with the 5% ethylacetate fraction of R. palmatum, respectively, compared with the control. The ethylacetate and BuOH fractions were also confirmed in greenhouse trials of rice seedlings infected by P. oryzae. The ethylacetate and BuOH fractions of R. palmatum ethanol extract were shown to have the potential for control of powdery mildew (Sphaerotheca fuliginea causes) and weeds, but not to have acaricidal activities against the two-spotted spider mite. The suppression rates of P. oryzae and P. capsici increased with increasing concentrations of chrysophanol, emodin, aloe-emodin, and rhein, but these extracts had no effect on cucumber powdery mildew or two-spotted spider mites. The anthraquinone levels in the ethylacetate fraction were much higher than in the BuOH fraction. Thus, the higher suppression of P. oryzae and P. capsici of the ethylacetate fraction observed in this study may be related to higher levels of anthraquinone substances in the ethylacetate fraction than the BuOH fraction.
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References
Agarwal SK, Singh SS, Verma S, Kumar S (2000) Antifungal activity of anthraquinone derivatives from Rheum emodi. J Ethnopharmacol 72:43–46
Bezabih M, Abegaz BM, Dufall K, Croft K, Skinner-Adams T, Davis TME (2001) Antiplasmodial and antioxidant isofuranonaphthoquinones from the roots of Bulbine capitata. Planta Med 67:340–344
Cho JR, Kim YJ, Ahn YJ, Yoo JK, Lee JO (1995) Monitoring of acaricide resistance in field-collected populations of Tetranychus urticae (Acari: Tetranychidae) in Korea. Korean J Appl Entomol 34:40–45
Choi GJ, Lee SW, Jang KS, Kim JS, Cho KY, Kim JC (2004) Effects of chrysophanol, parietin, and nepodin of Rumex crispus on barley and cucumber powdery mildew. Crop Prot 23:1215–1221
Choi KJ, Kim JC, Jang KS, Lim HK, Park IK, Shin SC, Cho KY (2006) In vivo antifungal activities of 67 plant fruit extracts against six plant pathogenic fungi. J Microbiol Biotechnol 16:491–495
Daaye F, Schmitt A, Belanger RR (1995) The effects of plant extracts of Reynoutria sachalinesis on powdery mildew development and leaf physiology of long cucumber. Plant Dis 79:577–580
Dayan FE, Cantrell CL, Duke SO (2009) Natural products in crop protection. Bioorgan Med Chem 17:4022–4034
Dik AJ, Van Der Staay M (1995) The effect of Milsana on cucumber powdery mildew under dutch conditions. Med Fac Landouwkundige Univ Gent 59:1027–1034
Gao LM, Wei XM, Zheng ZS (2002) Studies on chemical constituents of Rumex patientia. Chin Tradit Herbal Drugs 33:207–209
Gomiero T, Pimental D, Paoletti M (2011) Environmental impact of different agri-cultural management practices: conventional vs. organic agriculture. Crit Rev Plant Sci 30:95–124
Hahn M (2014) The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study. J Biol Chem 7:133–141
Huang ZS (1999) Control technology of major pests of vegetables in sunlight greenhouse: generation and control of Sphaerotheca fuliginea. J Veg 12:23
Inoue N, Nishimura H, Li HH, Mizutani J (1992) Allelochemicals from Polygonum sachalinense Fr, Schm. (Polygonaceae). J Chem Ecol 18:1833–1840
Izhaki I (2002) Emodin—a secondary metabolite with multiple ecological functions in higher plants. New Phytol 155:205–217
Jang SJ (2017) Control of diseases, insects, and weeds and growth promotion of crops by useful plant extracts. Ph.D. thesis. Sunchon National University. South Korea, p 205
Jang SJ, Yun YB, Kim SS, Kuk YI (2016) Suppression levels of crop pathogens and plants by extracts of medicinal plants containing chrysophanol and emodin. J Food Agric Environ 14:48–53
Kim JC, Choi KJ, Lee SW, Kim JS, Chung KY, Choi KY (2004) Screening extracts of Achyranthes japonica and Rumex crispus for activity against various plant pathogenic fungi and control of powdery mildew. Pest Manag Sci 60:803–808
Ko SK, Whang WK, Kim IH (1995) Anthraquinone and stilbene derivatives from cultivated Korean rhubarb rhizomes. Arch Pharm Res 18:282–288
Kwon CS, Lee SG (2002) Occurrence and ecological characteristics of red pepper anthracnose. Res Plant Dis 8:120–123
Lee JH (2001) The new rice farming science. Sunjinmunhwasa, Seoul, p 468
Li B, Liu BP, Yu RR, Lou MM, Wang YL, Xie GL, Li HY, Sun GC (2011) Phenotypic and molecular characterization of rhizobacterium Burkholderia sp. strain R456 antagonistic to Rhizoctonia solani, sheath blight of rice. World J Microbiol Biotechnol 27:2305–2313
Lin LC, Chou CJ, Kuo YC (2001) Cytotoxic principles from Ventilago leiocarpa. J Nat Prod 64:674–676
Mew TW, Gonzales P (2002) A handbook of rice seedborne fungi. International Rice Research Institute, Los Banós, p 83
Okabe H, Matsuo K, Noshioka I (1973) Studies on rhubarb (Rhei Rhizoma). II. Anthraquinone glycosides. Chem Pharm Bull 21:1254–1260
Padaria JC, Singh A (2009) Molecular characterization of soil bacteria antagonistic to Rhizoctonia solani, sheath blight of rice. J Environ Sci Health B 44:397–402
Rosslenbroich HJ, Stuebler D (2000) Botrytis cinerea history of chemical control and novel fungicides for its management. Crop Prot 19:557–561
Ryu SY, Kim JC, Kim HT, Kim SK, Choi GI, Kim JS, Lee SW, Heor J, Cho KY (2001) Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungi. Kor J Pestic Sci 5:26–35
Shan B, Cai YZ, Brooks JD, Corke H (2008) Antibacterial properties of Polygonum cuspidatum roots and their major bioactive constituents. Food Chem 109:530–537
Skamnioti P, Gurr SJ (2009) Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol 27:141–150
Statistical Analysis System (SAS) (2000) SAS/STAT user’s guide, version 7, Statistical Analysis System Institute, Electronic Version, Cary
Tang R, Zhang XH, Zhao JY (2003) Research development of cucumber powdery mildew. J Acta Agric North China Sin 18:68–70
Wang CM, Wu GB, Wang TZ, Shi ZQ (2005) The research of osthol against cucumber powdery mildew. Jiansu Agric Sci 4:57–58
Wang L, Bai L, Nagasawa T, Hasegawa T, Yang X, Sakai J, Bai Y, Kataoka T, Oka S, Hirose K, Tomida A, Tsuruo T, Ando M (2008) Bioactive triterpene saponins from the roots of Phytolacca americana. J Nat Prod 71:35–40
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This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01083903)” Rural Development Administration, Republic of Korea.
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Jang, S.J., Kuk, Y.I. Effects of different fractions of Rheum palmatum root extract and anthraquinone compounds on fungicidal, insecticidal, and herbicidal activities. J Plant Dis Prot 125, 451–460 (2018). https://doi.org/10.1007/s41348-018-0179-z
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DOI: https://doi.org/10.1007/s41348-018-0179-z