Journal of Plant Diseases and Protection

, Volume 125, Issue 4, pp 405–413 | Cite as

Morphological and molecular characterization of Colletotrichum nymphaeae and C. fructicola associated with anthracnose symptoms of grape in Santa Catarina State, southern Brazil

  • Claudia Aparecida Guginski-Piva
  • Amauri Bogo
  • Beatriz Ribeiro Gomes
  • Jessica Karine Menon
  • Rubens Onofre Nodari
  • Leocir José WelterEmail author
Original Article


Recent review described that grape anthracnose in Brazil is caused by Elsinoe ampelina. However, in many countries, anthracnose can be associated with different species of Colletotrichum. A group of 20 isolates obtained from leaves and shoots of grapes genotypes showing typical anthracnose symptoms were characterized using morphological criteria, species-specific PCR and internal transcribed spacer (ITS) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequencing. Two morphological types (I and II) were identified on the basis of colony appearance and conidial shape. Type I (Colletotrichum acutatum complex) produced colonies of flat growth salmon concentric rings and short cylindrical conidia measuring 13 × 5.73 µm. Type II (C. gloeosporioides complex) produced cotton growth white colonies and cylindrical conidia with both ends rounded and the same size as in type I. Phylogenetic trees based on Bayesian inference and using combined ITS and GAPDH sequence alignment exhibited five main clades, which represented the species of C. acutatum (C. nymphaeae) and C. gloeosporioides (C. fructicola) complexes. The standard isolates of type I CB-a (Cnymphaeae) and type II CB-g (Cfructicola) were pathogenic to potted vines of the Niagara cultivar 1 week after inoculation. This is the first report of the occurrence of Cnymphaeae and Cfructicola associated with grape anthracnose symptoms in Santa Catarina State, southern Brazil.


Vitis sp. Diagnostic PCR Internal transcribed spacer (ITS) Phylogenic analysis 



This study was funded by FAPESC (Santa Catarina State Foundation for Scientific and Technological Development—Grant Number TR-2012-0066), CNPq (The National Council for Scientific and Technological Development—Grant Number Edital 2015) and CAPES.

Compliance with ethical standards

Conflict of interest

All authors declare no conflict of interest.


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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2018

Authors and Affiliations

  • Claudia Aparecida Guginski-Piva
    • 1
  • Amauri Bogo
    • 1
  • Beatriz Ribeiro Gomes
    • 1
  • Jessica Karine Menon
    • 1
  • Rubens Onofre Nodari
    • 2
  • Leocir José Welter
    • 3
    Email author
  1. 1.Santa Catarina State UniversityLagesBrazil
  2. 2.Federal University of Santa CatarinaFlorianópolisBrazil
  3. 3.Federal University of Santa CatarinaCuritibanosBrazil

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