Journal of Plant Diseases and Protection

, Volume 125, Issue 3, pp 267–272 | Cite as

First detection and complete genome characterization of a Cherry (C) strain isolate of plum pox virus from sour cherry (Prunus cerasus) in Germany

  • Wilhelm Jelkmann
  • Daniel Sanderson
  • Constanze Berwarth
  • Delano James
Original Article
  • 32 Downloads

Abstract

An isolate of plum pox virus (PPV) was detected in sour cherry (Prunus cerasus) in the Eastern region of Germany. Symptoms observed on infected sour cherry included leaf chlorosis, vein clearing, leaf abnormality and dark spots and rings on fruit. The complete genome sequence of this PPV isolate (GC27) was determined. The virus genome consists of 9795 nucleotides (nt), excluding the poly(A) tail at the 3′ terminus, with 5′ and 3′ untranslated regions (UTRs) of 146 and 217 nt, respectively. Phylogenetic analysis based on the complete genome, the nucleotide sequence of the coat protein and the deduced amino acid sequence of the PIPO protein indicate that PPV isolate GC27 is a member of the Cherry (C) strain of PPV. In pairwise comparisons, the complete genome (nt) of isolate GC27 is 98.2–99.7% identical to other C isolates; with much lower identities for isolates of other strains including approximately 83% identity for isolates of the strain Cherry Russian. This is the first detection of a strain C isolate of PPV in Germany.

Keywords

PPV Strain C First detection Sour cherry Isolate GC27 Complete genome Molecular characterization 

Notes

Acknowledgements

The authors wish to thank Mrs. Ulrike Holz, Land Office for Rural Development, Agriculture and Land Reform, Plant Protection, Müllroser Chaussee 54, 15236 Frankfurt, Germany, for providing the PPV-infected leaf samples.

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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2018

Authors and Affiliations

  • Wilhelm Jelkmann
    • 1
  • Daniel Sanderson
    • 2
  • Constanze Berwarth
    • 1
  • Delano James
    • 2
  1. 1.Julius Kühn-Institute, Institute for Plant Protection in Fruit Crops and ViticultureDossenheimGermany
  2. 2.Sidney LaboratoryCanadian Food Inspection AgencyNorth SaanichCanada

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