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Journal of Plant Diseases and Protection

, Volume 125, Issue 3, pp 311–317 | Cite as

Colletotrichum coccodes in potato and tomato leaves in Russia

  • Grigoriy L. Belov
  • Arseniy F. Belosokhov
  • Irina A. Kutuzova
  • Natalia V. Statsyuk
  • Elena M. Chudinova
  • Alina V. Alexandrova
  • Lyudmila Y. Kokaeva
  • Sergey N. Elansky
Original Article

Abstract

Colletotrichum coccodes is a plant pathogenic fungus affecting different organs of potato, tomato, and some other plants. The leaf infection with C. coccodes may result in a rapid development of infection nidus and high losses caused by the infection of tomato fruits and potato tubers during harvesting. Our study showed the presence of C. coccodes on tomato and potato leaves with dry necrotic lesions. The presence of a DNA region specific for C. coccodes was detected in DNA samples isolated from infected tomato leaves collected in the Rostov region (7 and 1 samples from two distinct fields) and the Krasnodar Territory (5 samples from one field). In the case of potato leaf samples, the frequency of C. coccodes detection was lower than in tomato leaves. This fungus was revealed only in five potato leaf samples: one from the Northern Ossetia, one from the Kostroma region, and three from the Mariy El Republic. Pure cultures of two isolates were isolated from a naturally infected potato leaf. According to morphological criteria, size of spores and sclerotia, habitus of colonies, and species-specific DNA sequences, both strains completely corresponded to C. coccodes.

Keywords

Colletotrichum coccodes Anthracnose Black dot Potato diseases Tomato diseases 

Notes

Acknowledgements

This work was partially supported by the Russian Foundation for Basic Research (Project No. 15-29-02512). Arseniy Belosokhov and Lyudmila Kokaeva were supported by the Foundation for Assistance to Small Innovative Enterprises in Science and Technology.

References

  1. Alananbeh KM, Gudmestad NC (2016) Genetic diversity of Colletotrichum coccodes in the United States using amplified fragment length polymorphism analysis. J Gen Plant Pathol 82:199–211.  https://doi.org/10.1007/s10327-016-0662-y CrossRefGoogle Scholar
  2. Andrivon D, Lucas JM, Guerin C, Jouan B (1998) Colonization of roots, stolons, tubers, and stems of various potato (Solanum tuberosum) cultivars by the black-dot fungus Colletotrichum coccodes. Plant Pathol 47(4):440–445CrossRefGoogle Scholar
  3. Brierley JL, Stewart JA, Lees AK (2009) Quantifying potato pathogen DNA in soil. Appl Soil Ecol 41(2):234–238CrossRefGoogle Scholar
  4. Chesters CGC, Hornby D (1965) Studies on Colletotrichum coccodes. II. Alternative host tests and tomato fruit inoculations using a typical tomato root isolate. Trans Br Mycol Soc 48:583–594CrossRefGoogle Scholar
  5. Cullen DW, Lees AK, Toth IK, Duncan JM (2002) Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR. Plant Pathol 51(3):281–292CrossRefGoogle Scholar
  6. Dillard HR (1987) Tomato anthracnose. Fact sheet. New York State Agricultural Experiment Station. http://vegetablemdonline.ppath.cornell.edu/factsheets/Tomato_Anth.htm. Accessed 6 Feb 2017
  7. Dillard HR, Cobb AC (1997) Disease progress of black dot on tomato roots and reduction in incidence with foliar applied fungicides. Plant Dis 81(12):1439–1442CrossRefGoogle Scholar
  8. Gannibal PhB (2007) Early blight of potato leaves: species composition, taxonomy, and nomenclature of disease agents. Vestnik Zashchity Rasteniy (S):82–93 (in Russian)Google Scholar
  9. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Mol Ecol 2(2):113–118CrossRefPubMedGoogle Scholar
  10. Ingram J, Johnson DA (2010) Colonization of potato roots and stolons by Colletotrichum coccodes from tuber-borne inoculum. Am J Potato Res 87(4):382–389CrossRefGoogle Scholar
  11. Johnson DA (1994) Effect of foliar infection caused by Colletotrichum coccodes on yield of Russet Burbank potato. Plant Dis 78(11):1075–1078CrossRefGoogle Scholar
  12. Johnson DA, Miliczky ER (1993) Effects of wounding and wetting duration on infection of potato foliage by Colletotrichum coccodes. Plant Dis 77(1):13–17CrossRefGoogle Scholar
  13. Lewis Ivey ML, Nava-Diaz C, Miller SA (2004) Identification and management of Colletotrichum acutatum on immature bell peppers. Plant Dis 88(11):1198–1204CrossRefGoogle Scholar
  14. Liu F, Cai L, Crous PW, Damm U (2013) Circumscription of the anthracnose pathogens Colletotrichum lindemuthianum and C. nigrum. Mycologia 105(4):844–860CrossRefPubMedGoogle Scholar
  15. Mohan SK, Davis JR, Sorensen LH, Schneider AT (1992) Infection of aerial parts of potato plants by Colletotrichum coccodes and its effects on premature vine death and yield. Am Potato J 69(9):547–559CrossRefGoogle Scholar
  16. Nitzan N, Evans M, Johnson DA (2006) Colonization of potato plants after aerial infection by Colletotrichum coccodes, causal agent of potato black dot. Plant Dis 90(8):999–1003CrossRefGoogle Scholar
  17. Otazu V, Gudmestad NC, Zink RT (1978) The role of Colletotrichum atramentarium in the potato wilt complex in North Dakota. Plant Dis Report 62(10):847–851Google Scholar
  18. Raid RN, Pennypacker SP (1987) Weeds as hosts for Colletotrichum coccodes. Plant Dis 71:643–646CrossRefGoogle Scholar
  19. Tozze Júnior HJ, Firmino AC, Fischer IH, Furtado EL, Massola Júnior NS (2015) Characterization of Colletotrichum spp. isolates associated with fruit trees in the state of São Paulo. Summa Phytopathol 41(4):270–280CrossRefGoogle Scholar
  20. Tsror (Lakhim) L, Erlich O, Hazanovsky M (1999) Effect of Colletotrichum coccodes on potato yield, tuber quality and stem colonization during spring and autumn. Plant Dis 83(6):561–565CrossRefGoogle Scholar
  21. Tymon LS, Peever TL, Johnson DA (2016) Identification and enumeration of small-spored Alternaria species associated with potato in the U.S. Northwest. Plant Dis 100:465–472CrossRefGoogle Scholar
  22. White TJ, Bruns T, Lee SJWT, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR—protocols and applications—a laboratory manual. Academic Press, San Diego, pp 315–322Google Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2017

Authors and Affiliations

  • Grigoriy L. Belov
    • 1
  • Arseniy F. Belosokhov
    • 2
  • Irina A. Kutuzova
    • 3
  • Natalia V. Statsyuk
    • 5
  • Elena M. Chudinova
    • 4
    • 6
  • Alina V. Alexandrova
    • 2
  • Lyudmila Y. Kokaeva
    • 1
    • 2
  • Sergey N. Elansky
    • 2
    • 4
  1. 1.All-Russian Lorh Research Institute of Potato FarmingKraskovoRussia
  2. 2.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Agro-biological Center “Chashnikovo”Lomonosov Moscow State UniversityChashikovoRussia
  4. 4.Peoples Friendship University of Russia (RUDN University)MoscowRussia
  5. 5.All-Russian Research Institute of PhytopathologyBolshie VyazemyRussia
  6. 6.Institute of Protein Research of the Russian Academy of SciencesPushinoRussia

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