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Molecular and enzymatic approach to study Trichoderma harzianum-induced disease resistance in Brassica juncea against Albugo candida

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Abstract

Albugo candida, the causative agent of white rust disease in Indian mustard [Brassica juncea (L.) Czern. & Coss.], affects yield and quality of the crops. Biocontrol agents that induce disease resistance in crop plants against pathogen could be a reliable alternative to the chemical control. In this regard, we demonstrated the capabilities of Trichoderma harzianum, an efficient biocontrol agent to induce resistance in B. juncea against A. candida under green house conditions. Trichoderma harzianum treatment of Brassica seedlings followed by A. candida infection demonstrated an acquired resistance with an optimal disease control (45.6% disease reduction). This interaction exhibited an increase in antioxidant enzymes (glutathione reductase and peroxidase) and decreased in phosphatase activity. It also induced up-regulation of MAPK 6 and 17 genes. Hence, it is concluded that T. harzianum induced disease resistance in B. juncea against A. candida infection through the involvement of MAP kinase and up-regulation of antioxidant enzymes.

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Acknowledgements

The first author wishes to thank Department of Biotechnology (DBT), India, for financial support and Bhabha Atomic Research Centre (BARC) for providing facilities for carrying out research. The first author is thankful to the Dr. U. S. Singh and Dr. Anil K. Gupta GBPUA&T, Pantnagar, India, for providing the facilities necessary to conduct our initial research work. We are also thankful to Dr. (Mrs.) Meera Venkatesh and Dr. (Mrs.) Grace Samuel of Radiopharmaceuticals Division, BARC, for providing laboratory facilities and auxiliary staff to carry out radioactive experiments and molecular biology work.

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  1. Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India

    Priyadarshni Kumar

  2. Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    Chandan Kumar

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  1. Priyadarshni Kumar
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Correspondence to Priyadarshni Kumar.

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Kumar, P., Kumar, C. Molecular and enzymatic approach to study Trichoderma harzianum-induced disease resistance in Brassica juncea against Albugo candida . J Plant Dis Prot 125, 167–175 (2018). https://doi.org/10.1007/s41348-017-0137-1

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  • DOI: https://doi.org/10.1007/s41348-017-0137-1

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