Journal of Plant Diseases and Protection

, Volume 125, Issue 3, pp 287–296 | Cite as

Evaluation of a new natural adjuvant obtained from locust bean gum to reduce the amount of copper necessary to control downy mildew of grapevine

  • Ernesto LahozEmail author
  • Paola Tarantino
  • Pasquale Mormile
  • Mario Malinconico
  • Barbara Immirzi
  • Michele Cermola
  • Raffaele Carrieri
Original Article


Two field trials in integrated cultivation (2014 and 2016) were carried out with the aim to evaluate the efficacy and the persistence of copper oxychloride to control downy mildew of grapevine in mixture with a new natural adjuvant (PSS®) derived from locust bean (Ceratonia siliqua L.) gum. Copper amount applied in vineyards is the subject of interest for its use in organic farming due to the concern about its ecotoxicological profile in the soil that need to modify its use in rates, strategy and total amount per season. Copper oxychloride was applied every 7 and 14 days mixed or not with locust bean gum. Locust bean gum was also applied alone. To evaluate the distribution of copper on grapevine leaves, observations by scanning electron microscope were made. Locust bean gum alone did not influence the development of disease, while significantly increased efficacy and persistence of copper oxychloride both on leaves and bunches. On bunches, at 14 days interval, efficacy on incidence of copper oxychloride plus locust bean gum was about 60% while efficacy on severity was 60.1 and 65.6% for the year 2014 and 2016, respectively. With no locust bean gum, at the same interval, efficacy was significantly lower. Scanning electron microscopy demonstrated that copper fungicide particles when mixed with locust bean gum were still uniformly distributed on leaves after 14 days from application, but if applied alone already after 7 days became scattered with a lower concentration. To our knowledge, it is the first time that galactomannans-derived compounds have been used as adjuvant in pesticides. The use of locust bean gum reduced the annual amount of copper in integrated cultivation from 7.9 kg ha−1 applying copper oxychloride every 7 days to 4.5 kg ha−1 when applied every 14 days mixed with the new locust bean gum sticker.


Biodegradable sticker Downy mildew Grapevine SEM analysis Galactomannans Locust bean gum Plasmopara viticola Adjuvant Copper fungicides 



This study was performed thanks to the financial support of MAREA Project within Programma Operativo Nazionale “Ricerca e Competitività” 2007–2013 (PON R&C), PON.03PE_00106_1.


  1. Aziz A, Poinssot B, Daire X, Adrian M, Bézier A, Lambert B (2003) Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. Mol Plant Microbe Interact J 16:1118–1128CrossRefGoogle Scholar
  2. Cerqueira MR, Bourbon AI, Pinheiro AC, Martins JT, Souza BWS, Teixeira JA, Vicente AA (2011) Galactomannans use in the development of edible films/coatings for food applications. Trends Food Sci Technol 22:662–671CrossRefGoogle Scholar
  3. Feliziani E, Landi L, Romanazzi G (2015) Preharvest treatments with chitosan and other alternatives to conventional fungicides to control postharvest decay of strawberry. Carbohyd Polym 132:111–117CrossRefGoogle Scholar
  4. Ferreira ARV, Alves VD, Coelhoso IM (2016) Polysaccharide-based membranes in food packaging applications. Membranes 6(2):22CrossRefPubMedCentralGoogle Scholar
  5. Finckh MR, Tamm L, Bruns C (2015) Organic potato disease management. In: Finckh MR, van Bruggen AHC, Tamm L (eds) Plant diseases and their management in organic agriculture. APS Press, St Paul, pp 239–257Google Scholar
  6. Gent DH, Schwartz HF, Nissen SJ (2003) Effect of commercial adjuvants on vegetable crop fungicide coverage, absorption, and efficacy. Plant Dis 87:591–597CrossRefGoogle Scholar
  7. Grayson BT, Batten DM, Walter D (1996a) Adjuvant effects on the therapeutic control of potato late blight by dimethomorph wettable powder formulations. Pestic Sci 46:355–359CrossRefGoogle Scholar
  8. Grayson BT, Webb JD, Batten DM, Edwards D (1996b) Effects of adjuvants on the therapeutic activity of dimethomorph in controlling vine downy mildew. I. Survey of adjuvant types. Pestic Sci 46:199–206CrossRefGoogle Scholar
  9. Green JM, Beestman GB (2007) Recently patented and commericialized formulation and adjuvant technology. Crop Prot 26:320–327CrossRefGoogle Scholar
  10. Hart SE, Kells JJ, Penner D (1992) Influence of adjuvants on the efficacy, absorption. and spray retention of primsulfuron. Weed Technol 6:592–598CrossRefGoogle Scholar
  11. Kirkwood RC (1993) Use and mode of action of adjuvants for herbicides: a review of some current work. Pestic Sci 38:93–102CrossRefGoogle Scholar
  12. Krogh KA, Halling-Sorensen B, Mogensen BB, Vejrup KV (2003) Environmental properties and effects of non-ionic surfactant adjuvants in pesticides: a review. Chemosphere 50:871–901CrossRefPubMedGoogle Scholar
  13. Kudsk P, Mathiassen SK, Kirknel E (1991) Influence of formulations and adjuvants on the rainfastness of maneb and mancozeb on pea and potato. Pestic Sci 33:57–71CrossRefGoogle Scholar
  14. Lizzi Y, Coulomb C, Polian C, Coulomb PJ, Coulomb PO (1998) L’algue face au Mildiou quel avenir. Phytoma 508:29–30Google Scholar
  15. Loffaguen JC, Hartmann OEL, Talamini V, Stadnik MJ (2004) Extratos naturais no controle da antracnose e na produtividade do feijoeiro. Fitopatologia Brasileira 29(Suppl.):107Google Scholar
  16. Mackie KA, Müller T, Kandeler E (2012) Remediation of copper in vineyards: a mini review. Environ Pollut 167:16–26CrossRefPubMedGoogle Scholar
  17. Maschoff JR, Hart SE, Baldwin JL (2000) Effect of ammonium sulfate on the efficacy, absorption, and translocation of glufosinate. Weed Sci 48:2–6CrossRefGoogle Scholar
  18. OEPP, EPPO Bullettin (2014) Efficacy evaluation of plant protection products PP1/135(4). Phytotoxicity Assess 44(3):265–273Google Scholar
  19. OEPP/EPPO Bullettin (2001) Efficacy evaluation of plant protection products PP1/31(3). Plasmopara viticola. 31:305–310Google Scholar
  20. Orbovic V, Achor D, Syvertsen JP (2007) Adjuvants affect penetration of copper through isolated cuticles of Citrus leaves and fruit. Hort Sci 42:1405–1408Google Scholar
  21. Patier P, Potin P, Rochas C, Kloareg B, Yvin JC, Liénart Y (1995) Free or silica-bound oligokappa-carrageenans elicit laminarase activity in Rubus cells and protoplasts. Plant Sci 110:27–35CrossRefGoogle Scholar
  22. Percich JA, Nickelson LJ (1982) Evaluation of several fungicides and adjuvant materials for control of brown spot of wild rice. Plant Dis 66:1001–1003CrossRefGoogle Scholar
  23. Reddy KN, Locke MA (1996) Imazaquin spray retention, foliar washoff and runoff losses under simulated rainfall. Pestic Sci 48:179–187CrossRefGoogle Scholar
  24. Rogiers SY, Whitelaw-Weckert M, Radovanonic-Tesic M et al (2005) Effects of spray adjuvants on grape (Vitis vinifera) berry microflora, epicuticular wax and susceptibility to infection by Botrytis cinerea. Australas Plant Pathol 34:221CrossRefGoogle Scholar
  25. Rowen SS (1979) Evaluation of 13 adjuvants in combination with ferbam for control of fusiform rust on pine-seedlings. Plant Dis Rep 63:507–509Google Scholar
  26. Schutte GC, Kotze C, van Zyl JG, Fourie PH (2012) Assessment of retention and persistence of copper fungicides on orange fruit and leaves using fluorometry and copper residue analyses. Crop Prot 42:1–9CrossRefGoogle Scholar
  27. Steurbaut W (1993) Adjuvants for use with foliar fungicides. Pestic Sci 38:85–91CrossRefGoogle Scholar
  28. Stevens PJG (1993) Organosilicone surfactant as adjuvants for agrochemicals. Pestic Sci 38:103–122CrossRefGoogle Scholar
  29. Svenssons S, Svensson M (2010) Polysaccharide containing composition useful in forming protective film on surfaces selected from concrete, metal, stone, glass, wood, cloth, tissue, weave, paper. (Patent) PCT; International Publication Number WO2010/064982; 10 June 2010Google Scholar
  30. Van Zyl SA, Brink JC, Calitz FJ, Coertze S, Fourie PH (2010a) The use of adjuvants to improve spray deposition and Botrytis cinerea control on Chardonnay grapevine leaves. Crop Prot 29:58–67CrossRefGoogle Scholar
  31. Van Zyl SA, Brink JC, Calitz FJ, Fourie PH (2010b) Effects of adjuvants on deposition efficiency of fenhexamid sprays applied to Chardonnay grapevine foliage. Crop Prot 29:843–852CrossRefGoogle Scholar
  32. Xu L, Zhu H, Ozkan HE, Bagley WE (2010) Droplet evaporation and spread on waxy and hairy leaves associated with type and concentration of adjuvants. Pest Manag Sci 67:842–851CrossRefGoogle Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2017

Authors and Affiliations

  1. 1.CREA - Cereals and Industrial Crops Research CentreCasertaItaly
  2. 2.Institute of Applied Sciences and Intelligent Systems of CNRPozzuoliItaly
  3. 3.Institute of Polymer, Composites and Biomaterials of CNRPozzuoliItaly

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