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In vitro susceptibility of root-knot nematode populations (Meloidogyne spp.) from Crete, Greece to oxamyl and fenamiphos

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Abstract

Carbamate and organophosphate nematicides are routinely used for control of root-knot nematodes (RKN, Meloidogyne spp.) in greenhouses of Crete, Greece. Eleven populations of RKN were collected from greenhouses where such nematicides are intensively applied, but insufficient nematode control has been reported. Their susceptibility to fenamiphos and oxamyl was tested by estimating the percentage of immotile juveniles after 24-h exposure to five concentrations of each nematicide in 3.5-cm Petri dishes. Four populations collected from field crops without any recent history of nematicide application were also included in the study. Three populations maintained in pots for 3, 18 and 20 years without any exposure to nematicides served as controls. The estimation of LC50 and resistant factor values indicated that the nematode populations from greenhouses, despite their intense exposure to nematicides, did not have reduced susceptibility to fenamiphos and oxamyl compared to nematode populations maintained in pots.

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Acknowledgements

This research has been co‐financed by the European Union (European Social Fund—ESF) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF)—Research Funding Program: “THALES. Investing in knowledge society through the European Social Fund”. The collection of nematode populations was assisted by the agronomists A. Thomadakis, G. Troulinakis, D. Stavrianakis, M. Tzortzi and D. Sygelakis. We thank M. Grispou and M. Stavrakaki for computing data for analysis and Dr E. Roditakis for making useful comments on the manuscript.

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  1. Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture, N.AG.RE.F, Hellenic Agricultural Organization - DEMETER, 32A Kastorias street, Mesa Katsabas, 71307, Heraklion, Crete, Greece

    E. A. Tzortzakakis

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  1. E. A. Tzortzakakis
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Correspondence to E. A. Tzortzakakis.

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Tzortzakakis, E.A. In vitro susceptibility of root-knot nematode populations (Meloidogyne spp.) from Crete, Greece to oxamyl and fenamiphos. J Plant Dis Prot 124, 611–616 (2017). https://doi.org/10.1007/s41348-017-0107-7

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  • DOI: https://doi.org/10.1007/s41348-017-0107-7

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