Synthesis of copper oxide nanoparticles by chemical and biogenic methods: photocatalytic degradation and in vitro antioxidant activity

Abstract

Copper oxide nanoparticles (CuO-NPs) were synthesized using two different methods (chemical and biosynthesis) to study the influence of the preparation method on the structural, optical, morphological, photocatalyst, antibacterial and in vitro antioxidant of these nanoparticles. The synthesized nanoparticles were analysed by XRD, UV–Vis, HR-TEM, DLS, ZE, PL and FT-IR spectroscopy. The X-ray diffraction spectra showed the single-phase monoclinic structure of copper oxide, with an average crystallite size of 2.05–3.00 nm. HR-TEM analysis confirmed the spherical morphology of the synthesized CuO-NPs using chemical and biological methods with an average size of 32 nm and 25 nm, respectively. The synthesized CuO-NPs exhibited potential photocatalytic activity towards the degradation of methylene blue dye on exposing to sunlight irradiation. The degradation effectiveness against methylene blue dye was found to be 85 and 97% for chemical and biosynthesized CuO-NPs, respectively. Furthermore, antibacterial and antioxidant activities were evaluated. The biogenic method showed a significant antibacterial activity against Gram-negative bacteria E. coli and B. subtilis than Gram-positive bacteria and also DPPH assay.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

References

  1. 1.

    Linic S, Aslam U, Boerigter C, Morabito M (2015) Photochemical transformations on plasmonic metal nanoparticles. Nat Mater 14:567–576

    Article  Google Scholar 

  2. 2.

    Muthuvel A, Jothibas M, Manoharan C (2020) Effect of chemically synthesis compared to biosynthesized ZnO-NPs using Solanum nigrum leaf extract and their photocatalytic, antibacterial and in-vitro antioxidant activity. J Environ Chem Eng 8:103705

    Article  Google Scholar 

  3. 3.

    Arunkumar B, Johnson Jeyakumar S, Jothibas M (2019) A sol-gel approach to the synthesis of CuO nanoparticles using Lantana camara leaf extract and their photo catalytic activity. Optik 183:698–705

    Article  Google Scholar 

  4. 4.

    Vinothkumar P, Manoharan C, Shanmugapriya B, Bououdina M (2019) Effect of reaction time on structural, morphological, optical and photocatalytic properties of copper oxide (CuO) nanostructures. J Mater Sci Mater El 30:6249–6262

    Article  Google Scholar 

  5. 5.

    Rafique M, Shafiq F, Ali Gillani SS, Shakil M, Tahir MB, Sadaf I (2019) Eco-friendly green and biosynthesis of copper oxide nanoparticles using Citrofortunella microcarpa leaves extract for efficient photocatalytic degradation of Rhodamin B dye form textile wastewater. Optik. https://doi.org/10.1016/j.ijleo.2019.164053

    Article  Google Scholar 

  6. 6.

    Tahir MB (2019) Microbial photoelectrochemical cell for improved hydrogen evolution using nickel ferrite incorporated WO3 under visible light irradiation. Int J Hydrog Energy 44:17316–17322

    Article  Google Scholar 

  7. 7.

    Ahmad R, Mondal PK (2012) Adsorption and photodegradation of methylene blue by using PAni/TiO2 nanocomposite. J Disper Sci Technol 33:380–386

    Article  Google Scholar 

  8. 8.

    Ahmad R, Kumar R (2011) Synthesis and properties of cellulose carbon encapsulated ZnO for dye removal. J Disper Sci Technol 32:737–740

    Article  Google Scholar 

  9. 9.

    Tahir MB, Kiran H, Iqbal T (2019) The detoxification of heavy metals from aqueous environment using nano-photocatalysis approach: a review. Environ Sci Pollut 26:10515–10528

    Article  Google Scholar 

  10. 10.

    Kumar R, Mondal PK, Ahmad R (2014) Adsorptive removal of hazardous methylene blue by fruit shell of Cocos nucifera. Environ Eng Manag J 13:231–240

    Article  Google Scholar 

  11. 11.

    Tahir MB, Nabi G, Rafique M, Khalid NR (2017) Nanostructured-based WO3 photocatalysts: recent development, activity enhancement, perspectives and applications for wastewater treatment. Int J Environ Sci Tech 14:2519–2542

    Article  Google Scholar 

  12. 12.

    Bilal Tahir M, Nabi G, Rafique M, Khalid NR (2018) Role of fullerene to improve the WO3 performance for photocatalytic applications and hydrogen evolution. Int J Energy Res 42:4783–4789

    Article  Google Scholar 

  13. 13.

    Bilal Tahir M, Nadeem Riaz K, Asiri AM (2019) Boosting the performance of visible light-driven WO3/g-C3N4 anchored with BiVO4 nanoparticles for photocatalytic hydrogen evolution. Int J Energy Res 43:5747–5758

    Article  Google Scholar 

  14. 14.

    Dorner L, Cancellieri C, Rheingans B, Walter M, Kagi R, Schmutz P, Kovalenko MV, Jeurgens LPH (2019) Cost-effective sol-gel synthesis of porous CuO nanoparticle aggregates with tunable specific surface area. Sci Rep 9:1

    Article  Google Scholar 

  15. 15.

    Khan A, Rashid A, Younas R, Chong R (2015) A chemical reduction approach to the synthesis of copper nanoparticles. Int Nano Lett 6:21–26

    Article  Google Scholar 

  16. 16.

    Phiwdang K, Suphankij S, Mekprasart W, Pecharapa W (2013) Synthesis of CuO nanoparticles by precipitation method using different precursors. Energy Proc 34:740–745

    Article  Google Scholar 

  17. 17.

    Felix S, Chakkravarthy RBP, Grace AN (2015) Microwave assisted synthesis of copper oxide and its application in electrochemical sensing. IOP Conf Ser Mater Sci Eng 73:012115

    Article  Google Scholar 

  18. 18.

    Rani P, Siril PF, Srivastava R (2017) Cu nanoparticles decorated Cu organic framework based efficient and reusable heterogeneous catalyst for coupling reactions. Mol Catal 433:100–110

    Article  Google Scholar 

  19. 19.

    Singh J, Dutta T, Kim K-H, Rawat M, Samddar P, Kumar P (2018) Green synthesis of metals and their oxide nanoparticles: applications for environmental remediation. J Nanobiotechnol 16:1

    Article  Google Scholar 

  20. 20.

    Jayakumarai G, Gokulpriya C, Sudhapriya R, Sharmila G, Muthukumaran C (2015) Phytofabrication and characterization of monodisperse copper oxide nanoparticles using Albizia lebbeck leaf extract. Appl Nanosci 5:1017–1021

    Article  Google Scholar 

  21. 21.

    Nasrollahzadeh M, Sajadi SM, Khalaj M (2014) Green synthesis of copper nanoparticles using aqueous extract of the leaves of Euphorbia esula L and their catalytic activity for ligand-free Ullmann-coupling reaction and reduction of 4-nitrophenol. RSC Adv 4:47313–47318

    Article  Google Scholar 

  22. 22.

    Abboud Y, Saffaj T, Chagraoui A, El Bouari A, Brouzi K, Tanane O, Ihssane B (2013) Biosynthesis, characterization and antimicrobial activity of copper oxide nanoparticles (CONPs) produced using brown alga extract (Bifurcaria bifurcata). Appl Nanosci 4:571–576

    Article  Google Scholar 

  23. 23.

    Sebeia N, Jabli M, Ghith A (2019) Biological synthesis of copper nanoparticles, using Nerium oleander leaves extract: characterization and study of their interaction with organic dyes. Inorg Chem Commun 105:36–46

    Article  Google Scholar 

  24. 24.

    Ghareib M, Abdallah W, Abu Tahon M, Tallima A (2019) Biosynthesis of copper oxide nanoparticles using the preformed biomass of aspergillus fumigatus and their antibacterial and photocatalytic activities. Dig J Nanomater Bios 14(2019):291–303

    Google Scholar 

  25. 25.

    Das P, Ghosh S, Ghosh R, Dam S, Baskey M (2018) Madhuca longifolia plant mediated green synthesis of cupric oxide nanoparticles: a promising environmentally sustainable material for waste water treatment and efficient antibacterial agent. J Photochem Photobiol B 189:66–73

    Article  Google Scholar 

  26. 26.

    Hai Le Tu (2019) Biosynthesis, characterization and photocatalytic activity of copper/copper oxide nanoparticles produced using aqueous extract of Lemongrass Leaf. Compos Mater 3:30–35

    Google Scholar 

  27. 27.

    Sathiyavimal S, Vasantharaj S, Bharathi D, Saravanan M, Manikandan E, Kumar SS, Pugazhendhi A (2018) Biogenesis of copper oxide nanoparticles (CuONPs) using Sida acuta and their incorporation over cotton fabrics to prevent the pathogenicity of Gram negative and Gram positive bacteria. J Photochem Photobiol B 188:126–134

    Article  Google Scholar 

  28. 28.

    Naika HR, Lingaraju K, Manjunath K, Kumar D, Nagaraju G, Suresh D, Nagabhushana H (2015) Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity. J Taibah Univ Sci 9:7–12

    Article  Google Scholar 

  29. 29.

    Sengottaiyan A, Aravinthan A, Sudhakar C, Selvam K, Srinivasan P, Govarthanan M, Manoharan K, Selvankumar T (2015) Synthesis and characterization of Solanum nigrum-mediated silver nanoparticles and its protective effect on alloxan-induced diabetic rats. J Nanostruct Chem 6:41–48

    Article  Google Scholar 

  30. 30.

    Muthuvel A, Adavallan K, Balamurugan K, Krishnakumar N (2014) Biosynthesis of gold nanoparticles using Solanum nigrum leaf extract and screening their free radical scavenging and antibacterial properties. Biomed Prev Nutr 4:325–332

    Article  Google Scholar 

  31. 31.

    Suganya S, Jothibas M, Muthuvel A (2019) Effect of temperature on different properties of ZnS nanoparticles synthesized by solid-state reaction method. J Nanosci Nanotechnol 4:787–790

    Google Scholar 

  32. 32.

    Das D, Nath BC, Phukon P, Kalita A, Dolui SK (2013) Synthesis of ZnO nanoparticles and evaluation of antioxidant and cytotoxic activity. Colloid Surf B 111:556–560

    Article  Google Scholar 

  33. 33.

    Muthuvel A, Jothibas M, Manoharan C, Jayakumar SJ (2020) Synthesis of CeO2-NPs by chemical and biological methods and their photocatalytic, antibacterial and in vitro antioxidant activity. Res Chem Intermediat 46:2705–2729

    Article  Google Scholar 

  34. 34.

    Loganayaki N, Siddhuraju P, Manian S (2011) Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. J Food Sci Tech 50:687–695

    Article  Google Scholar 

  35. 35.

    Son H, Yen P (2014) Preliminary phytochemical screening, acute oral toxicity and anticonvulsant activity of the berries of Solanum nigrum Linn. Trop J Pharm Res 13:907

    Article  Google Scholar 

  36. 36.

    Din MI, Arshad F, Hussain Z, Mukhtar M (2017) Green adeptness in the synthesis and stabilization of copper nanoparticles: catalytic, antibacterial, cytotoxicity, and antioxidant activities. Nanoscale Res Lett 12:1

    Article  Google Scholar 

  37. 37.

    Jacobs H, Moalin M, Bast A, van der Vijgh WJF, Haenen GRMM (2010) An Essential Difference between the Flavonoids MonoHER and quercetin in their interplay with the endogenous antioxidant network. PLoS ONE 5:e13880

    Article  Google Scholar 

  38. 38.

    Gharibshahi E, Saion E (2012) Influence of dose on particle size and optical properties of colloidal platinum nanoparticles. Int J Mol 13:14723–14741

    Article  Google Scholar 

  39. 39.

    Vaidehi D, Bhuvaneshwari V, Bharathi D, Sheetal BP (2018) Antibacterial and photocatalytic activity of copper oxide nanoparticles synthesized using Solanum lycopersicum leaf extract. Mater Res Express 8:085403

    Article  Google Scholar 

  40. 40.

    Priya RS, Geetha D, Ramesh PS (2016) Antioxidant activity of chemically synthesized AgNPs and biosynthesized Pongamia pinnata leaf extract mediated AgNPs—a comparative study. Ecotox Environ Safe 134:308–318

    Article  Google Scholar 

  41. 41.

    Thurner C, Debbage P (2018) Molecular imaging with nanoparticles: the dwarf actors revisited 10 years later. Histochem Cell Biol 150:733–794

    Article  Google Scholar 

  42. 42.

    Narasaiah P, Mandal BK, Sarada NC (2017) Biosynthesis of copper Oxide nanoparticles from Drypetes sepiaria Leaf extract and their catalytic activity to dye degradation. IOP Conf Ser Mater Sci Eng 263:022012

    Article  Google Scholar 

  43. 43.

    Chand Mali S, Raj S, Trivedi R (2019) Biosynthesis of copper oxide nanoparticles using Enicostemma axillare (Lam.) leaf extract. B B Rep 20:100699

    Google Scholar 

  44. 44.

    Ramesh M, Anbuvannan M, Viruthagiri G (2015) Green synthesis of ZnO nanoparticles using Solanum nigrum leaf extract and their antibacterial activity. Spectrochim Acta A 136:864–870

    Article  Google Scholar 

  45. 45.

    Senthilkumar N, Nandhakumar E, Priya P, Soni D, Vimalan M, Vetha Potheher I (2017) Synthesis of ZnO nanoparticles using leaf extract of Tectona grandis (L.) and their anti-bacterial, anti-arthritic, anti-oxidant and in vitro cytotoxicity activities. New J Chem 41:10347–10356

    Article  Google Scholar 

  46. 46.

    Ihsan M, Niaz A, Rahim A, Zaman MI, Arain MB, Sirajuddin S, Sharif T, Najeeb M (2015) Biologically synthesized silver nanoparticle-based colorimetric sensor for the selective detection of Zn2+. RSC Adv 5:91158–91165

    Article  Google Scholar 

  47. 47.

    Handago DT, Zereffa EA, Gonfa BA (2019) Effects of Azadirachta Indica leaf extract, capping agents, on the synthesis of pure and Cu doped ZnO nanoparticles: a green approach and microbial activity. Open Chem 17:246–253

    Article  Google Scholar 

  48. 48.

    Knop S, Jansen TLC, Lindner J, Vohringer P (2011) On the nature of OH-stretching vibrations in hydrogen-bonded chains: pump frequency dependent vibrational lifetime. Phys Chem Chem Phys 13:4641

    Article  Google Scholar 

  49. 49.

    Jadhav MS, Kulkarni S, Raikar P, Barretto DA, Vootla SK, Raikar US (2018) Green biosynthesis of CuO & Ag–CuO nanoparticles from Malus domestica leaf extract and evaluation of antibacterial, antioxidant and DNA cleavage activities. New J Chem 42:204–213

    Article  Google Scholar 

  50. 50.

    Cuevas R, Duran N, Diez MC, Tortella GR, Rubilar O (2015) Extracellular biosynthesis of copper and copper oxide nanoparticles by Stereum hirsutum, a native White-Rot fungus from Chilean forests. J Nanomater 2015:1–7

    Article  Google Scholar 

  51. 51.

    Saif S, Tahir A, Asim T, Chen Y (2016) Plant Mediated green synthesis of CuO nanoparticles: comparison of toxicity of engineered and plant mediated CuO nanoparticles towards Daphnia magna. Nanomater 6:205

    Article  Google Scholar 

  52. 52.

    Anwaar S, Maqbool Q, Jabeen N, Nazar M, Abbas F, Nawaz B, Hussain T, Hussain SZ (2016) The effect of green synthesized CuO nanoparticles on callogenesis and regeneration of Oryza sativa L. Front Plant Sci. https://doi.org/10.3389/fpls.2016.01330

    Article  Google Scholar 

  53. 53.

    Dagher S, Haik Y, Ayesh AI, Tit N (2014) Synthesis and optical properties of colloidal CuO nanoparticles. J Lumin 151:149–154

    Article  Google Scholar 

  54. 54.

    Thammavongsy Z, Mercer IP, Yang JY (2019) Promoting proton coupled electron transfer in redox catalysts through molecular design. Chem Comm 55:10342–10358

    Article  Google Scholar 

  55. 55.

    Malleshappa J, Nagabhushana H, Sharma SC, Vidya YS, Anantharaju KS, Prashantha SC, Prasad BD, Naika HR, Lingaraju K, Surendra BS (2015) Leucas aspera mediated multifunctional CeO2 nanoparticles: structural, photoluminescent, photocatalytic and antibacterial properties. Spectrochim Acta A 149:452–462

    Article  Google Scholar 

  56. 56.

    Das P, Ghosh S, Ghosh R, Dam S, Baskey M (2018) Madhuca longifolia plant mediated green synthesis of cupric oxide nanoparticles: a promising environmentally sustainable material for waste water treatment and efficient antibacterial agent. J Photochem Photobiol B 89:66–73

    Article  Google Scholar 

  57. 57.

    Slavin YN, Asnis J, Hafeli UO, Bach H (2017) Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J Nanobiotechnol 15:1

    Article  Google Scholar 

  58. 58.

    Raghupathi KR, Koodali RT, Manna AC (2011) size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir 27:4020–4028

    Article  Google Scholar 

  59. 59.

    Jeong Y, Lim DW, Choi J (2014) Assessment of size-dependent antimicrobial and cytotoxic properties of silver nanoparticles. Adv Mater Sci Eng 2014:1–6

    Article  Google Scholar 

  60. 60.

    Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK (2018) Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechol 9:1050–1074

    Article  Google Scholar 

  61. 61.

    Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4:118

    Article  Google Scholar 

  62. 62.

    Shanmugam C, Sivasubramanian G, Parthasarathi B, Baskaran K, Balachander R, Parameswaran VR (2015) Antimicrobial, free radical scavenging activities and catalytic oxidation of benzyl alcohol by nano-silver synthesized from the leaf extract of Aristolochia indica L.: a promenade towards sustainability. Appl Nanosci 6:711–723

    Article  Google Scholar 

  63. 63.

    Fang M, Chen J, Xu X, Yang P, Hildebrand H (2006) Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests. Int J Antimicrob Agents 27:513–517

    Article  Google Scholar 

  64. 64.

    Uhl L, Gerstel A, Chabalier M, Dukan S (2015) Hydrogen peroxide induced cell death: one or two modes of action. Heliyon 1:e00049

    Article  Google Scholar 

  65. 65.

    Chung I, Abdul Rahuman A, Marimuthu S, Vishnu Kirthi A, Anbarasan K, Padmini P, Rajakumar G (2017) Green synthesis of copper nanoparticles using Eclipta prostrata leaves extract and their antioxidant and cytotoxic activities. Exp Ther Med. https://doi.org/10.3892/etm.2017.4466

    Article  Google Scholar 

  66. 66.

    Ijaz F, Shahid S, Khan SA, Ahmad W, Zaman S (2017) Green synthesis of copper oxide nanoparticles using Abutilon indicum leaf extract: antimicrobial, antioxidant and photocatalytic dye degradation activities. Trop J Pharm Res 16:743

    Article  Google Scholar 

  67. 67.

    Rajeshkumar S, Rinitha G (2018) Nanostructural characterization of antimicrobial and antioxidant copper nanoparticles synthesized using novel Persea americana seeds. OpenNano 3:18–27

    Article  Google Scholar 

  68. 68.

    Rehana D, Mahendiran D, Kumar RS, Rahiman AK (2017) Evaluation of antioxidant and anticancer activity of copper oxide nanoparticles synthesized using medicinally important plant extracts. Biomed Pharmacother 89:1067–1077

    Article  Google Scholar 

Download references

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Affiliations

Authors

Corresponding author

Correspondence to M. Jothibas.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Muthuvel, A., Jothibas, M. & Manoharan, C. Synthesis of copper oxide nanoparticles by chemical and biogenic methods: photocatalytic degradation and in vitro antioxidant activity. Nanotechnol. Environ. Eng. 5, 14 (2020). https://doi.org/10.1007/s41204-020-00078-w

Download citation

Keywords

  • CuO-NPs
  • Biosynthesis
  • Solanum nigrum
  • Photocatalytic
  • Antibacterial