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Harnessing Symbiotic Association of Lactic Acid Bacteria and Cellulose-Synthesizing Bacteria for Enhanced Biological Activity

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Abstract

Bacterial cellulose (BC) is an extremely pure and highly valuable biomaterial. However, its production cost poses a challenge for large-scale manufacturing. This study explores a cost-effective approach by co-cultivating lactic acid bacteria with cellulose-synthesizing bacteria. Four BC-producing isolates from spoiled fruits and four lactic acid bacteria from fermented buttermilk were isolated and characterized. Growth studies demonstrated successful lactic acid bacteria cultivation in HS media. Co-cultivation of cellulose-synthesizing bacteria and lactic acid bacteria showed enhanced BC production, with a twofold increase in dry weight (0.35 g/150 ml) compared to the monoculture of cellulose-synthesizing bacteria (0.15 g/150 ml). Scanning electron microscopy revealed improved BC microfibril quality in co-culture. Reversed-phase HPLC confirmed higher lactic acid concentrations in co-culture. 16S rRNA sequence analysis revealed that lactic acid bacteria had a 100% match with Lactococcus lactis. These findings highlight the potential of co-cultivation for cost-effective BC production and lactic acid yield, offering a sustainable approach to biomaterial production.

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Abbreviations

BC:

Bacterial cellulose

MRS:

DeMan, Rogosa and Sharpe

HS:

Hestrin and Schramm

LAB:

Lactic acid bacteria

CSB:

Cellulose-synthesizing bacteria

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Acknowledgements

The authors would like to express their gratitude to the PG Department of Biotechnology at Alva’s College for providing all essential materials and equipment for carrying out this work.

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This research received no external funding.

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Authors and Affiliations

  1. PG Department of Biotechnology, Alva’s College, Moodbidri, D.K, Karnataka, 574227, India

    Ali Jawad Akki, Lavanya D. Hiremath & Raghavendra Rao Badkillaya

  2. Faculty of Science and Technology, BLDE (Deemed-to-be University), Vijayapura, Karnataka 586103, India

    Ali Jawad Akki

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  1. Ali Jawad Akki
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  2. Lavanya D. Hiremath
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  3. Raghavendra Rao Badkillaya
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Correspondence to Raghavendra Rao Badkillaya.

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Akki, A.J., Hiremath, L.D. & Badkillaya, R.R. Harnessing Symbiotic Association of Lactic Acid Bacteria and Cellulose-Synthesizing Bacteria for Enhanced Biological Activity. Iran J Sci 48, 311–320 (2024). https://doi.org/10.1007/s40995-023-01567-8

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