Increasing concerns about the emergence of antibiotic-resistant bacteria have drawn much attention for research efforts to introduce new types of antibacterial compounds among scientists. In the present report, 24 bacterial strains isolated from the coastal areas of the Persian Gulf in Iran were investigated in order to assess their potentials to produce antibacterial metabolites. Among these, the crude extract from the isolate REB264 showed promising inhibition activity on the growth of two pathogenic bacteria, Pseudomonas aeruginosa and Staphylococcus aureus. Molecular identification via sequencing of the amplified 16S rDNA gene of the strain REB264 revealed that the bacterium belonging to the genus Bacillus and was tentatively named Bacillus sp. REB264, while the sequence was deposited in GenBank database, NCBI. Optimization of the carbon and nitrogen sources of the strain REB264 culture media determined that 743 mg/l active extract was achieved when using 1% glycerol and 0.5% yeast extract as carbon and nitrogen sources, respectively. Crude extract purification to obtain the antibacterial metabolite was performed in three steps using fractionation in organic and aqueous solvents, silica gel 60 column chromatography and LH-20 gel filtration with the yield of 80%. The purified compound exhibited inhibitory effect on P. aeruginosa growth at minimum inhibitory concentration (MIC) of 15 µg/ml, while lacking antibacterial effect on S. aureus. The P. aeruginosa growth inhibitor 264 (PAGI264) compound was further purified using reversed-phase high-performance liquid chromatography (C18-HPLC) up to 95% and the structural analysis by FTIR and H1-NMR confirmed that PAGI264 is composed of alcoholic, carbonyl and phenolic functional groups. Moreover, MS data in positive mode showed that the molecular weight of the compound is 761.74 Da. Lack of toxicity to MCF-7 cell line up to 500 µg/ml and lack of the hemolytic effect on red blood cells showed that the present natural product, PAGI264, has capability to use as a lead or drug in the future.
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This work was financially supported by the research affairs of Shiraz University, Shiraz, Iran under Grant Code of 97GCU2M143077.
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Ebrahimi, R., Pournejati, R. & Karbalaei-Heidari, H.R. Pseudomonas aeruginosa Growth Inhibitor, PAGI264: A Natural Product from a Newly Isolated Marine Bacterium, Bacillus sp. Strain REB264. Iran J Sci Technol Trans Sci (2021). https://doi.org/10.1007/s40995-021-01107-2
- Antimicrobial agents
- Pseudomonas aeruginosa
- Natural products
- Marine Bacillus