Modeling of In Vitro Biomass Production of Digitalis purpurea Under the Effects of Biosynthetic Silver Nanoparticles


Nanoparticles are being used in many areas in biotechnology. Silver nanoparticles are the most frequently used ones among the others, because of their eco-friendly natures. Biosynthetic silver nanoparticles have some beneficial effects on plant tissue cultures. However, their effects have rarely been investigated. In this study, biosynthetic nanoparticles were used at 0, 1, 2 and 4 mg/L concentration in order to determine their effects on Digitalis purpurea node cultures. At the first stage of experimental data analyses, MANOVA was employed to understand the significant growth parameters of D. purpurea plantlets. At the second stage, an artifical neural network (ANN) model was designed and tested under a wide range of input parameters (concentration of silver nanoparticles). The statistical differences between shoot elongations, single and multiple shoot formations, root formations, root fresh and dry weights were not found significant. However, the difference between shoot and total fresh weights, shoot and total dry weights (the significant variables) were found statistically significant. Optimum silver nanoparticle concentration for plantlet growth was detected as 2 mg/L. Biomass accumulations were enhanced up to 2,4 times. The results showed that ANN model provided a good prediction for the plant growth parameters and can be adapted for in vitro large-scale production.

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Data Availability

All data were given in the manuscript and the references parts.

Code Availability

STATISTICA v.15 software for MANOVA and Matlab R2019a for ANN.


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This study was not supported by a foundation.

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All laboratory experiments were performed by PN and AT. Statistical analysis were performed by ADA. Paper preparation were performed by PN and ADA.

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Correspondence to Pınar Nartop.

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Nartop, P., Altan, A.D. & Titrek, A. Modeling of In Vitro Biomass Production of Digitalis purpurea Under the Effects of Biosynthetic Silver Nanoparticles. Iran J Sci Technol Trans Sci 45, 775–783 (2021).

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  • Digitalis purpurea
  • in vitro biomass production
  • Biosynthetic silver nanoparticles
  • Syzygium aromaticum
  • Artifical neural network