Abstract
Wild pansy (Viola tricolor L.) is of the Violaceae plant family. Some of the medicinal properties of this plant are related to the ability to produce and biosynthesized a group of flavonoids. Silver nanoparticles are one of the most important and attractive nanomaterials among several metal nanoparticles.In the present study, plant-mediated nanosilver was produced from aqueous extract of Pansy flower as a good covering and stabilizing agent using the green synthesis method. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and zeta potential methods were used to determine the specifications of synthesized nanoparticles. In this study, the effect of different concentrations of bio nanosilver 0, 10, 50, and 100 mg/l on phytochemical and physiological properties, and the expression of some key genes involved in flavonoids biosynthesis including phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavonoid 3′, 5′-hydroxylase (F3′5′H), and flavonoid 3′-hydroxylase (F3′H) were determined in V. tricolor plant. The results showed nanosilver treatments cause the accumulation of manganese, zinc, and silver elements and increasing the content of phenolic, flavonoid, anthocyanin and antioxidant activity. Using AgNPs also causes the increase in activity of antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), phenylalanine ammonia-lyase (PAL), and peroxidase (POD). Besides, treatment with a concentration of 10 mg/l of silver nanoparticles significantly causes an increase in flavonoids compounds such as rutin, apigenin, and quercetin. Our results suggest that bio nanosilver could increase secondary metabolites content in the V. tricolor plant.
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Notes
SEM.
TEM.
Zeta potential.
DLS.
References
Adefegha SA, Oyeleye SI, Dada FA et al (2018) Modulatory effect of quercetin and its glycosylated form on key enzymes and antioxidant status in rats penile tissue of paroxetine-induced erectile dysfunction. Biomed Pharmacother 107:1473–1479. https://doi.org/10.1016/j.biopha.2018.08.128
Agarwal H, Venkat Kumar S, Rajeshkumar S (2018) Antidiabetic effect of silver nanoparticles synthesized using lemongrass (Cymbopogon citratus) through conventional heating and microwave irradiation approach. J MicrobiolBiotechnol Food Sci 7:371–376. https://doi.org/10.15414/jmbfs.2018.7.4.371-376
Ali SW, Rajendran S, Joshi M (2011) Synthesis and characterization of chitosan and silver loaded chitosan nanoparticles for bioactive polyester. CarbohydrPolym 83:438–446. https://doi.org/10.1016/j.carbpol.2010.08.004
Babu TS, Akhtar TA, Lampi MA et al (2003) Similar stress responses are elicited by copper and ultraviolet radiation in the aquatic plant Lemna gibba: implication of reactive oxygen species as common signals. Plant Cell Physiol 44:1320–1329. https://doi.org/10.1093/pcp/pcg160
Bailly C, Benamar A, Corbineau F, Côme D (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiol Plant 97:104–110. https://doi.org/10.1111/j.1399-3054.1996.tb00485.x
Bais HP, Sudha GS, Ravishankar GA (2000) Putrescine and silver nitrate influences shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknow local. J Plant Growth Reg 19:238–248. https://doi.org/10.1007/s003440000012
Baskar V, Venkatesh J, Park SW (2015) Impact of biologically synthesized silver nanoparticles on the growth and physiological responses in Brassica rapa ssp. pekinensis. Environ SciPollut Res 22:17672–17682. https://doi.org/10.1007/s11356-015-4864-1
Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M (2016) Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. ApplNanosci 6:755–766. https://doi.org/10.1007/s13204-015-0473-z
Binder BM (2020) Ethylene signaling in plants. J BiolChem 295:7710–7725
Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Ann Rev Plant Biol 54:519–546. https://doi.org/10.1146/annurev.arplant.54.031902.134938
Casillas-figueroa F, Arellano-garcía ME, Leyva-aguilera C et al (2020) ArgovitTM silver nanoparticles effects on allium cepa: plant growth promotion without cytogenotoxic damage. Nanomaterials 10:1–20. https://doi.org/10.3390/nano10071386
Chand K, Cao D, Fouad DE et al (2020) Green synthesis, characterization and photocatalytic application of silver nanoparticles synthesized by various plant extracts. Arab J Chem. https://doi.org/10.1016/j.arabjc.2020.01.009
Ebrahimzadeh MA, Nabavi SM, Nabavi SF et al (2010) Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J Pharm Sci 23:29–34. https://doi.org/10.1080/14786419.2012.658799
Gan Q, Wang T, Cochrane C, McCarron P (2005) Modulation of surface charge, particle size and morphological properties of chitosan–TPP nanoparticles intended for gene delivery. Coll Surf B Biointerf 44:65–73. https://doi.org/10.1016/j.colsurfb.2005.06.001
Garcia-Brugger A, Lamotte O, Vandelle E et al (2006) Early signaling events induced by elicitors of plant defenses. Molecul Plant Microbe Interact 19:711–724. https://doi.org/10.1094/MPMI-19-0711
Ghorbani A, Razavi SM, Omran VOG, Pirdashti H (2018) Piriformosporaindica alleviates salinity by boosting redox poise and antioxidative potential of tomato. Russ J Plant Physiol 65:898–907. https://doi.org/10.1134/S1021443718060079
Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59:309–314. https://doi.org/10.1104/pp.59.2.309
Gokce Y, Cengiz B, Yildiz N et al (2014) Ultrasonication of chitosan nanoparticle suspension: influence on particle size. Coll Surf APhysicochemEng Aspects 462:75–81. https://doi.org/10.1016/j.colsurfa.2014.08.028
Guardia T, Rotelli AE, Juarez AO, Pelzer LE (2001) Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Il Farmaco 56:683–687. https://doi.org/10.1016/S0014-827X(01)01111-9
Hong J, Rico CM, Zhao L et al (2015) Toxic effects of copper-based nanoparticles or compounds to lettuce (Lactuca sativa) and alfalfa (Medicago sativa). Environ Sci Process Impacts 17:177–185. https://doi.org/10.1039/c4em00551a
Janbaz KH, Saeed SA, Gilani AH (2002) Protective effect of rutin on paracetamol-and CCl4-induced hepatotoxicity in rodents. Fitoterapia 73:557–563. https://doi.org/10.1016/S0367-326X(02)00217-4
Jiang H-S, Qiu X-N, Li G-B et al (2014) Silver nanoparticles induced accumulation of reactive oxygen species and alteration of antioxidant systems in the aquatic plant Spirodela polyrhiza. Environ ToxicolChem 33:1398–1405. https://doi.org/10.1002/etc.2577
Jurca T, Pallag A, Marian E, Eugenia M (2019) The histo-anatomical investigation and the polyphenolic profile of antioxidant complex active ingredients from three viola species. Farmacia 67:634–640. https://doi.org/10.31925/farmacia.2019.4.12
Kamalakkannan N, Prince PSM (2006) Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic wistar rats. Basic ClinPharmacolToxicol 98:97–103. https://doi.org/10.1111/j.1742-7843.2006.pto_241.x
Keshari AK, Srivastava R, Singh P et al (2020) Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. J Ayurveda Integr Med 11:37–44. https://doi.org/10.1016/j.jaim.2017.11.003
Kim J-E, Joo S-I, Seo J-H, Lee S-P (2009) Antioxidant and α-glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food SciNutr 38:989–995. https://doi.org/10.3746/jkfn.2009.38.8.989
Koda T, Kuroda Y, Imai H (2008) Protective effect of rutin against spatial memory impairment induced by trimethyltin in rats. Nutr Res 28:629–634. https://doi.org/10.1016/j.nutres.2008.06.004
Kosyk OI, Khomenko IM, Batsmanova LM, Taran NY (2017) Phenylalanine ammonia-lyase activity and anthocyanin content in different varieties of lettuce under the cadmium influence. UkrBiochem J. https://doi.org/10.17721/1728_2748.2018.75.37-45
Krizek DT, Britz SJ, Mirecki RM (1998) Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cv. New Red Fire lettuce Physiol Plant 103:1–7. https://doi.org/10.1034/j.1399-3054.1998.1030101.x
La Casa C, Villegas I, De La Lastra CA et al (2000) Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmacol 71:45–53. https://doi.org/10.1016/S0378-8741(99)00174-9
Matsui K, Walker AR (2020) Biosynthesis and regulation of flavonoids in buckwheat. Breed Sci 70:74–84. https://doi.org/10.1270/jsbbs.19041
Mattioli R, Francioso A, Mosca L, Silva P (2020) Anthocyanins: a comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules 25:3809. https://doi.org/10.3390/molecules25173809
Mehrian SK, Heidari R, Rahmani F (2015) Effect of silver nanoparticles on free amino acids content and antioxidant defense system of tomato plants. Indian J Plant Physiol 20:257–263. https://doi.org/10.1007/s40502-015-0171-6
Munné-Bosch S, Peñuelas J, Asensio D, Llusià J (2004) Airborne ethylene may alter antioxidant protection and reduce tolerance of holm oak to heat and drought stress. Plant Physiol 136:2937–2947. https://doi.org/10.1104/pp.104.050005
Nabavi SM, Šamec D, Tomczyk M et al (2020) Flavonoid biosynthetic pathways in plants: versatile targets for metabolic engineering. BiotechnolAdv 38:107316. https://doi.org/10.1016/j.biotechadv.2018.11.005
Nair PMG, Chung IM (2014) Physiological and molecular level effects of silver nanoparticles exposure in rice (Oryza sativa L.) seedlings. Chemosphere 112:105–113. https://doi.org/10.1016/j.chemosphere.2014.03.056
Nijveldt RJ, Van Nood ELS, Van Hoorn DEC et al (2001) Flavonoids: a review of probable mechanisms of action and potential applications. Am J ClinNutr 74:418–425. https://doi.org/10.1093/ajcn/74.4.418
Papadimitriou S, Bikiaris D, Avgoustakis K et al (2008) Chitosan nanoparticles loaded with dorzolamide and pramipexole. CarbohydrPolym 73:44–54. https://doi.org/10.1016/j.carbpol.2007.11.007
Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucl Acids Res 29:e45–e45. https://doi.org/10.1093/nar/29.9.e45
Raei M, Angaji SA, Omidi M, et al (2014) Effect of abiotic elicitors on tissue culture of Aloe vera. Int J Biosci 5:74–81. https://doi.org/10.12692/ijb/5.1.74-81.
Rice-Evans C, Miller N, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
Saunders JA, McClure JW (1974) The suitability of a quantitative spectrophotometric assay for phenylalanine ammonia-lyase activity in barley, buckwheat, and pea seedlings. Plant Physiol 54:412–413. https://doi.org/10.1104/pp.54.3.412
Servin AD, Morales MI, Castillo-Michel H et al (2013) Synchrotron verification of TiO2 accumulation in cucumber fruit: a possible pathway of TiO2 nanoparticle transfer from soil into the food chain. Environ SciTechnol 47:11592–11598. https://doi.org/10.1021/es403368j
Shahzad A, Saeed H, Iqtedar M et al (2019) Size-controlled production of silver nanoparticles by aspergillusfumigatus BTCB10: likely antibacterial and cytotoxic effects. J Nanomater. https://doi.org/10.1155/2019/5168698
Sharma P, Bhatt D, Zaidi MGH et al (2012) Silver nanoparticle-mediated enhancement in growth and antioxidant status of brassica juncea. ApplBiochemBiotechnol 167:2225–2233. https://doi.org/10.1007/s12010-012-9759-8
Smirnoff N (1996) Botanical briefing: the function and metabolism of ascorbic acid in plants. Ann Bot 78:661–669. https://doi.org/10.1006/anbo.1996.0175
Sohal JK, Saraf A, Shukla K, Shrivastava M (2019) Determination of antioxidant potential of biochemically synthesized silver nanoparticles using Aloe vera gel extract. Plant Sci Today 6:208–217. https://doi.org/10.14719/pst.2019.6.2.532
Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess BiosystEng 32:79. https://doi.org/10.1007/s00449-008-0224-6
Song JY, Jang H-K, Kim BS (2009) Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts. Process Biochem 44:1133–1138. https://doi.org/10.1016/j.procbio.2009.06.005
Tang L, Kwon S-Y, Kim S-H et al (2006) Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature. Plant Cell Rep 25:1380–1386. https://doi.org/10.1007/s00299-006-0199-1
Trujillo-Reyes J, Majumdar S, Botez CE et al (2014) Exposure studies of core: shell Fe/Fe3O4 and Cu/CuO NPs to lettuce (Lactuca sativa) plants: are they a potential physiological and nutritional hazard? J Hazard Mater 267:255–263. https://doi.org/10.1016/j.jhazmat.2013.11.067
Umashankari J, Inbakandan D, Ajithkumar TT, Balasubramanian T (2012) Mangrove plant, Rhizophoramucronata (Lamk, 1804) mediated one pot green synthesis of silver nanoparticles and its antibacterial activity against aquatic pathogens. AquatBiosyst 8:1–7. https://doi.org/10.1186/2046-9063-8-11
Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117. https://doi.org/10.1021/jf9801973
Vukics V, Kery A, Bonn GK, Guttman A (2008) Major flavonoid components of heartsease (Viola tricolor L.) and their antioxidant activities. Anal BioanalChem 390:1917–1925. https://doi.org/10.1007/s00216-008-1885-3
Wagner GJ (1979) Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiol 64:88–93. https://doi.org/10.1104/pp.64.1.88
Yi TG, Yeoung YR, Choi I-Y, Park N-I (2019) Transcriptome analysis of Asparagus officinalis reveals genes involved in the biosynthesis of rutin and protodioscin. PLoS ONE 14:e0219973. https://doi.org/10.1371/journal.pone.0219973
Zaeem A, Drouet S, Anjum S, et al (2020) Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. in vitro cultures: a comparative analysis. Biomolecules 10:918. doi:https://doi.org/10.3390/biom10060918.
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. BiotechnolAdv 23:283–333. https://doi.org/10.1016/j.biotechadv.2005.01.003
Zuverza-Mena N, Armendariz R, Peralta-Videa JR, Gardea-Torresdey JL (2016) Effects of silver nanoparticles on radish sprouts: root growth reduction and modifications in the nutritional value. Front Plant Sci 7:90. https://doi.org/10.3389/fpls.2016.00090
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The author thanks the Razi Herbal Medicine Research Center of the Lorestan University of Medical Science for their contribution to implementing the project.
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AH was involved in data curation, writing—original draft, conceptualization, methodology, resources, formal analysis. SS contributed to review and editing, supervision, project administration. HLY was involed in validation, supervision, project administration. AI contributed to software, supervision, project administration.
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Hassanvand, A., Saadatmand, S., Lari Yazdi, H. et al. Biosynthesis of NanoSilver and Its Effect on Key Genes of Flavonoids and Physicochemical Properties of Viola tricolor L.. Iran J Sci Technol Trans Sci 45, 805–819 (2021). https://doi.org/10.1007/s40995-021-01091-7
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DOI: https://doi.org/10.1007/s40995-021-01091-7