Elicitation Improves Phenolic Acid Content and Antioxidant Enzymes Activity in Salvia leriifolia Cell Cultures

Abstract

Elicitors, such as salicylic acid (SA), stand as the effective agents that are applied to plant cell suspension cultures for the fabrication of biomaterials. At this project, we have studied the elicitor effects on the increase in phenols, PAL activities, POX, and CAT enzymes that exist in the cell culture of Salvia leriifolia. Upon the addition of SA to the cell culture, an increase has been detected in the phenols (CA and Sal B) and the activity of PAL, POX, and CAT enzymes. The increases of phenols and PAL activity have been stimulated 48 h after being treated with SA and the activities of resulting antioxidant enzymes have been detected to be greatly improved. Elicitation of SA on phenols gathering has been dependent on the applied dose and time period. The appropriate SA assemblage for eliciting phenols accumulations has been determined to be 10 mg/L. Elicitation influence of SA on phenols gatherings has associated with the rate of PAL, indicating that PAL may be the crucial role for the synthesis of Sal B and CA. The increased PAL activity can lead to the progress of phenolic compounds amount, which is quite significant in regards to the usage of cell culture systems for biofabrication of secondary metabolites such as CA and Sal B.

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Abbreviations

SA:

Salicylic acid

PAL:

Phenyl Alanine Ammonialyase

POX:

Peroxidase

CAT:

Catalase

CA:

Caffeic Acid

Sal B:

Salvianolic acid B

NAA:

Naftalen Acetic Acid

BAP:

Benzil Amino Purin

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Acknowledgements

The authors thank the Farhangian University of Mashhad for providing the required equipments and facilities.

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Correspondence to Masoomeh Modarres or Mohammad Ehsan Taghavizadeh Yazdi.

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The authors declare that they have no conflict of interest.

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Modarres, M., Taghavizadeh Yazdi, M.E. Elicitation Improves Phenolic Acid Content and Antioxidant Enzymes Activity in Salvia leriifolia Cell Cultures. Iran J Sci Technol Trans Sci 45, 849–855 (2021). https://doi.org/10.1007/s40995-021-01070-y

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Keywords

  • Salvia leriifolia
  • Elicitation
  • Cell culture
  • Salicylic acid
  • Phenols
  • Antioxidant enzymes