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Cytogenetic and Micro-Morphological Studies on Several Accessions of Some Lepidium L. Species in Iran

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Abstract

We investigated genome size and stomatal characteristics of guard cells of several Iranian accessions belonging to three Lepidium species. Average 2C DNA of L. sativum and L. latifolium accessions was estimated at 1.23 and 2.07 pg, respectively, and all accessions were diploid. 2C value of L. draba accessions was in the range of 1.11–2.29 pg. Results of cluster analysis on the basis of nuclear 2C DNA showed that L. draba accessions had a relationship with geographic origin, unlike L. sativum and L. latifolium. Stomatal density and chloroplast number of guard cells differed among accessions of L. draba, ranging 29.2–52.7 stomata/mm2 and 9.3–16.9, respectively. In L. draba, a negative and positive correlation observed between stomatal density and chloroplast number of guard cells with nuclear DNA content, respectively. There is a substantial variation in stomatal characteristics among accessions that could be useful for the selection and breeding of Lepidium for adaptation to different climates.

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Authors and Affiliations

  1. Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Afra Roughani & Vahid Abdossi

  2. Department of Horticulture, Karaj Branch, Islamic Azad University, Azadi St., Eram Blvd., Mehrshahr, Box 31876/44511, Karaj, Iran

    Seied Mehdi Miri

  3. Department of Horticulture, University of Tehran, Karaj, Iran

    Mohammad Reza Hassandokht

  4. Department of Horticulture, Saveh Branch, Islamic Azad University, Saveh, Iran

    Pejman Moradi

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  1. Afra Roughani
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  2. Seied Mehdi Miri
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Correspondence to Seied Mehdi Miri.

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Roughani, A., Miri, S.M., Hassandokht, M.R. et al. Cytogenetic and Micro-Morphological Studies on Several Accessions of Some Lepidium L. Species in Iran. Iran J Sci Technol Trans Sci 45, 417–426 (2021). https://doi.org/10.1007/s40995-020-01035-7

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