Abstract
Diabetes mellitus is associated with increased oxidative stresses, kidney damage, ocular neuropathy, and atherosclerosis. In the present study, the protective effect of Rubus fruticosus hydroethanolic extract on the biochemical parameters in streptozotocin (STZ)-induced diabetic rats were investigated. In this experimental study, 40 male Wistar rats were divided into five groups (n = 8) as control, diabetic (STZ-injection 55 mg kg−1 of body weight, intraperitoneally (i.p.)), and treatment 1, 2, and 3 which treated by R. fruticosus extract (50, 100 and 200 mg kg−1, i.p.). After 4 weeks, the animals were deeply anesthetized and their blood samples were collected from the heart directly for the measurement of biochemical parameters and inflammatory cytokines. The findings indicate that the serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), ceruloplasmin (Cp), copper (Cu), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 increased significantly after the onset of diabetes (P < 0.001 for all assayed parameters). Treatment by R. fruticosus extract at high dose (200 mg kg−1) reduced the serum levels of ALT, AST, ALP, GGT, Cp, Cu, TNF-α, and IL-6 (P < 0.001, for all parameters) significantly compared to the diabetic group. Overall, the administration of the R. fruticosus extract can ameliorate some of the adverse effects of STZ-induced diabetes among the male rats that received this treatment.
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Acknowledgements
The authors of this article are aware of the gratitude and appreciation of the high-ranking officials of the Vice-Chancellor for Research and Technology, and the graduates of the Islamic Azad University of Damghan, as well as, the staff of the medical diagnostic laboratory of the city of Hamedan for their comprehensive assistance and cooperation.
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Soheilifar, M., Shiravi, A., Mirazi, N. et al. Modulatory Effect of Rubus fruticosus L. on Liver Biochemical Metabolism and Inflammatory Cytokines in the Diabetic Rats. Iran J Sci Technol Trans Sci 45, 103–110 (2021). https://doi.org/10.1007/s40995-020-01001-3
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DOI: https://doi.org/10.1007/s40995-020-01001-3