How is the Effect of Silver Nanoparticles and Lipase/Cellulase Enzymes on Each Other?

Abstract

Different modifications have existed in the textile industry since its creation. Among these is the use of a variety of compounds that improve properties and lifetime as well as new washing methods. Washing materials containing enzymes (i.e., lipase and cellulase) are used to improve the strength of fibers, remove stains, increase the surface hydrolysis, and dissolve particles. Silver nanoparticles (AgNPs) in the form of co-spinning with or deposition on textiles are used to increase their antibacterial and self-cleansing properties. However, it should be noted that the interaction between AgNPs and proteins causes the formation of the protein corona on its surface and decreases both properties. This study examined the effects of AgNPs, lipase and cellulase enzymes as detergent enzymes on the antimicrobial and cleaning features, respectively. For this purpose, the three quantitative factors of enzyme–nanoparticle ratio, temperature, and time and the qualitative factor of enzyme type (cellulase or lipase) were monitored using the central composite design (CCD). The lipase and cellulase and antimicrobial properties of the AgNPs were assayed using different analytical methods, including UV–Vis, dynamic light scattering, X-ray powder diffraction, circular dichroism, 1D gel electrophoresis, contact angle, and bacterial inhibition zone against Staphylococcus aureus and Escherichia coli. It was found that the interaction of AgNPs decreased the activity of the lipase enzyme, but the activity level of the cellulase enzyme was not significantly changed. The antibacterial properties of AgNPs are also mainly inhibited through interaction with lipase.

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Acknowledgement

Financial support from the Research Council of Shahid Beheshti University is gratefully acknowledged. This publication represents a component of the MSc thesis of Behnam Eisazadeh at Shahid Beheshti University, Tehran, Iran. The authors declare no conflicts of interest.

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Correspondence to Fateme Mirzajani.

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Eisazadeh, B., Mirzajani, F. & Sefidbakht, Y. How is the Effect of Silver Nanoparticles and Lipase/Cellulase Enzymes on Each Other?. Iran J Sci Technol Trans Sci 44, 27–35 (2020). https://doi.org/10.1007/s40995-020-00820-8

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Keywords

  • Cellulase
  • CCD
  • Lipase
  • Silver nanoparticle