Abstract
Changes in environmental salinity are responsible for a variety of physiological stress responses in aquatic organisms. Induced stress is associated with enhanced reactive oxygen species generation, which caused oxidative damage. In the present study, the effects of different environmental salinities on the antioxidant status in the gills of the yellowfin seabream, Acanthopagrus sheim, were assayed. Fish adapted to salinity of 20 ppt for 14 days, then suddenly challenged with experimental salinities including 5, 12 and 34 ppt and kept in new salinities for 14 days. Each treatment was done in three replicates. In each replicate, 12 fishes with an average initial weight of 100 ± 10 g were placed in 300-L tanks. Fish were fed 3 times daily 3% body weight. Gill samples were taken after 2 weeks. Catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity and malondialdehyde (MDA) content were assayed in gill tissue. CAT, SOD and GPX activity did not show a significant difference between experimental groups. Like antioxidant enzymes, MDA content of gill was not significantly different between experimental groups. Our finding showed that salinity challenge as a stressor did not affect antioxidant–prooxidant equilibrium or a new equilibrium was created and did not evoke oxidative stress.
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References
Aebi H (1984) Catalase in vitro. Method Enzymol 105:121–126
Aksnes A, Njaa LR (1981) Catalase, glutathione peroxidase and superoxide dismutase in different fish species. Comp Biochem Physiol 69B:893–896. https://doi.org/10.1016/0305-0491(81)90402-8
An MI, Choi CY (2010) Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: effects on hemolymph and biochemical parameters. Comp Biochem Physiol 155B:34–42. https://doi.org/10.1016/j.cbpb.2009.09.008
An KW, Kim NN, Shin HS, Kil GS, Choi CY (2010) Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli). Comp Biochem Physiol 156A:262–268. https://doi.org/10.1016/j.cbpa.2010.02.013
Aras NM, Bayir A, Sirkecioglu AN, Bayir M, Aksakal E, Haliloglu HI (2009) Seasonal changes in antioxidant defense system of liver and gills of Salmo trutta caspius, Salmo trutta labrax and Salmo trutta macrostigma. J Fish Biol 74:842–856. https://doi.org/10.1111/j.1095-8649.2008.02164.x
Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Method Enzymol 52:302–310. https://doi.org/10.1016/S0076-6879(78)52032-6
Choi CY, An KW, An MI (2008) Molecular characterization and mRNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder (Paralichthys olivaceus). Comp Biochem Physiol 149A:330–337. https://doi.org/10.1016/j.cbpa.2008.01.013
Eskandari G, Saghavi H, Zabayeh Najafabadi M, Dehghan Madiseh S, Koochaknejad E (2013) Effect of salinity on reproductive performance of Acanthopagrus latus (Houttuyn) in spawning tanks. Aquac Res 44:588–595. https://doi.org/10.1111/j.1365-2109.2011.03062.x
Evans DH, Piermarini PM, Choe KP (2005) The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85:97–177. https://doi.org/10.1152/physrev.00050.2003
Farshadian R, Salati AP, Keyvanshokooh S, Pasha-Zanoosi H, Negin Taji A (2017) Effect of short-term exposure with different salinity levels on the gill histology in Yellowfin Seabream (Acanthopagrus latus). Anim Res J (Accepted for publication)
Hwang PP, Lee TH, Lin LY (2011) Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms. Am J Physiol (Regul Integr Comp Physiol) 301:28–47. https://doi.org/10.1152/ajpregu.00047.2011
Inokuchi M, Kaneko T (2012) Recruitment and degeneration of mitochondrion-rich cells in the gills of Mozambique tilapia Oreochromis mossambicus during adaptation to a hyperosmotic environment. Comp Biochem Physiol 162A:245–251. https://doi.org/10.1016/j.cbpa.2012.03.018
Iwatsuki Y (2013) Review of the Acanthopagrus latus complex (Perciformes: Sparidae) with descriptions of three new species from the Indo-West Pacific Ocean. J Fish Biol 83:64–95. https://doi.org/10.1111/jfb.12151
Karimi S, Kochinian P, Salati AP (2013) The effect of sexuality on some hematological parameters of the yellowfin seabream, Acanthopagrus latus in Persian Gulf. Iran J Vet Res 14(1):65–68. https://doi.org/10.22099/IJVR.2013.1392
Karimi S, Kochinian P, Salati AP, Gooraninejad S (2014) Plasma sex steroids and Gonadosomatic index variations during ovarian development of female wild yellowfin seabream (Acanthopagrus latus). Ichthyol Res 61:258–264. https://doi.org/10.1007/s10228-013-0378-3
Kashiwagi A, Kashiwagi K, Takase M, Hanada H, Nakamura M (1997) Comparison of catalase in diploid and haploid Rana rugosa using heat and chemical inactivation techniques. Comp Biochem Physiol 118B:499–503. https://doi.org/10.1016/S0305-0491(97)00216-2
Liu Y, Wang W, Wang A, Wang J, Sun R (2007) Effects of dietary vitamin E supplementation on antioxidant enzyme activities in Litopenaeus vannamei (Boone, 1931) exposed to acute salinity changes. Aquaculture 265:351–358. https://doi.org/10.1016/j.aquaculture.2007.02.010
Loro VL, Jorge MB, Rios da Silva K, Wood CM (2012) Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus: protective effects of salinity. Aquat Toxicol 110–111:187–193. https://doi.org/10.1016/j.aquatox.2012.01.012
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lushchak VI (2011) Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 101:13–30. https://doi.org/10.1016/j.aquatox.2010.10.006
McCord JM, Fridovich I (1969) Superoxide dismutase: an enzymic function for erythrocuprein. J Biol Chem 244:6049–6055
Najafi A, Salati AP, Yavari V, Asadi F (2014) Effects of short-term starvation and refeeding on antioxidant defense in Mesopotamichthys sharpeyi (Günther, 1874) fingerlings. Int J Aquat Biol 5:242–246
Nakabō T (2002) Fishes of Japan: with pictorial keys to the species. Tokai University Press, Tokyo
Paital B, Chainy GBN (2010) Antioxidant defenses and oxidative stress parameters in tissues of mud crab (Scylla serrata) with reference to changing salinity. Comp Biochem Physiol 151C:142–151. https://doi.org/10.1016/j.cbpc.2009.09.007
Rodrigues AP, Correia Oliveira P, Guilhermino L, Guimaraes L (2012) Effects of salinity stress on neurotransmission, energy metabolism, and anti-oxidant biomarkers of Carcinus maenas from two estuaries of the NW Iberian Peninsula. Mar Biol 159:2061–2074. https://doi.org/10.1016/j.aquatox.2014.08.011
Ross SW, Dalton DA, Kramer S, Christensen BL (2001) Physiological (antioxidant) responses of estuarine fishes to variability in dissolved oxygen. Comp Biochem Physiol 130C:289–303. https://doi.org/10.1016/S1532-0456(01)00243-5
Sangiao-Alvarellos S, Laiz-Carrion R, Guzman JM, Martin del Rio MP, Miguez JM, Mancera JM, Soengas JL (2003) Acclimation of S. aurata to various salinities alters energy metabolism of osmoregulatory and nonosmoregulatory organs. Am J Physiol 285:R897–R907. https://doi.org/10.1152/ajpregu.00161.2003
Taghipoor K, Keyvanshokooh S, Salati AP, Pasha-Zanoosi H, Bahrami Babaheydari S (2016) Effects of triploidy induction on antioxidant defense status in rainbow trout (Oncorhynchus mykiss) during early development. Anim Reprod Sci 171:108–113. https://doi.org/10.1016/j.anireprosci.2016.06.005
Vernet P, Rock E, Mazur A, Rayssiguier Y, Dufaure JP, Drevet JR (1999) Selenium-independent epididymis-restricted glutathione peroxidase 5 protein (GPX5) can back up failing Se-dependent GPXs in mice subjected to selenium deficiency. Mol Reprod Dev 54:362–370. https://doi.org/10.1002/(SICI)1098-2795(199912)54:4
Wilson JM, Laurent P (2002) Fish gill morphology: inside out. J Exp Zool 293:192–213. https://doi.org/10.1002/jez.10124
Zhao F, Zhuang P, Zhang LZ (2008) Responses of antioxidases in different tissues of Acipenser schrenckii to increased salinity in water. Mar Fish Res 29:65–69. https://doi.org/10.3969/j.issn.1000-7075.2008.05.010
Zikos A, Seale AP, Lerner DT, Grau EG, Korsmeyer KE (2014) Effects of salinity on metabolic rate and branchial expression of genes involved in ion transport and metabolism in Mozambique tilapia (Oreochromis mossambicus). Comp Biochem Physiol 178A:121–131. https://doi.org/10.1016/j.cbpa.2014.08.016
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Ghanavatinasab, Y., Salati, A.P., Movahedinia, A. et al. Changes in Gill Antioxidant Status in Acanthopagrus sheim Exposed to Different Environmental Salinities. Iran J Sci Technol Trans Sci 43, 1479–1483 (2019). https://doi.org/10.1007/s40995-018-0663-0
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DOI: https://doi.org/10.1007/s40995-018-0663-0