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Synthesis of Silver Nanoparticles by Clinacanthus nutans Extract Supported with Identification of Flavonoids by UPLC-QTOF/MS and Its Antimicrobial Activity

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Abstract

The present study reported a simple method in synthesizing silver nanoparticles (AgNPs) by using Clinacanthus nutans extract. The UV–visible spectra showed the characteristic absorption peak at 480 nm, and the intensity was increased with the increase in plant extract ratio and incubation period. Analysis of particle size through FESEM, XRD and TEM revealed the average of synthesized AgNPs is 73.4 nm. The EDX analysis confirmed the formation of metallic nature of silver. The FTIR spectra indicated the role of carbonyl groups in the synthetic process and further confirmed by identification of flavonoids and their glycosides by UPLC-QTOF/MS. Antimicrobial activity of biosynthesized AgNPs shows effective inhibition against common bacterial strains including Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris.

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References

  • Ahmed S, Ahmad M, Swami BL, Ikram S (2016) A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 7:17–28

    Article  Google Scholar 

  • Alam A, Ferdosh S, Ghafoor K, Hakim A, Juraimi AS, Khatib A, Sarker ZI (2016) Clinacanthus nutans: a review of the medicinal uses, pharmacology and phytochemistry. Asian Pac J Trop Med 9:402–409

    Article  Google Scholar 

  • Bindhu M, Umadevi M (2013) Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity. Spectrochim Acta Part A Mol Biomol Spectrosc 101:184–190

    Article  Google Scholar 

  • Cao Y, Jin R, Mirkin CA (2001) DNA-modified core − shell Ag/Au nanoparticles. J Am Chem Soc 123:7961–7962

    Article  Google Scholar 

  • Gong P et al (2007) Preparation and antibacterial activity of Fe3O4@ Ag nanoparticles. Nanotechnology 18:285604

    Article  Google Scholar 

  • Hamid HA, Mutazah R, Yusoff MM, Karim NAA, Razis AFA (2017) Comparative analysis of antioxidant and antiproliferative activities of Rhodomyrtus tomentosa extracts prepared with various solvents. Food Chem Toxicol 108:451–457

    Article  Google Scholar 

  • Hamid HA, Ramli ANM, Zamri N, Yusoff MM (2018) UPLC-QTOF/MS-based phenolic profiling of Melastomataceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231. Food Chem 265:253–259

    Article  Google Scholar 

  • Hazrulrizawati H, Zeyohannes SS (2017) Rhodomyrtus Tomentosa: a phytochemical and pharmacological review. Asian J Pharm Clin Res 10:1–7

    Google Scholar 

  • López-Esparza J, Espinosa-Cristóbal LNF, Donohue-Cornejo A, Reyes-López SNY (2016) Antimicrobial activity of silver nanoparticles in polycaprolactone nanofibers against gram-positive and gram-negative bacteria. Ind Eng Chem Res 55:12532–12538

    Article  Google Scholar 

  • Lu J, Bravo-Suárez JJ, Takahashi A, Haruta M, Oyama ST (2005) In situ UV–Vis studies of the effect of particle size on the epoxidation of ethylene and propylene on supported silver catalysts with molecular oxygen. J Catal 232:85–95

    Article  Google Scholar 

  • Makarov V, Love A, Sinitsyna O, Makarova S, Yaminsky I, Taliansky M, Kalinina N (2014) “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae (aнглoязычнaя вepcия) 6

  • Panáček A et al (2006) Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B 110:16248–16253

    Article  Google Scholar 

  • P’ng XW, Akowuah GA, Chin JH (2012) Acute oral toxicity study of Clinacanthus nutans in mice. Int J Pharm Sci Res 3:4202

    Google Scholar 

  • Rana S, Kalaichelvan P (2011) Antibacterial activities of metal nanoparticles. Antibact Act Metal Nanoparticles 11:21–23

    Google Scholar 

  • Shah S, Ukaegbu C, Hamid H, Alara O (2018) Evaluation of antioxidant and antibacterial activities of the stems of Flammulina velutipes and Hypsizygus tessellatus (white and brown var.) extracted with different solvents. J Food Meas Charact 12:1947–1961

    Article  Google Scholar 

  • Standards NCFCL, Jorgensen JH (1993) Performance standards for antimicrobial disk susceptibility tests. National Committee for Clinical Laboratory Standards

  • Sun Y, Xia Y (2003) Gold and silver nanoparticles: a class of chromophores with colors tunable in the range from 400 to 750 nm. Analyst 128:686–691

    Article  Google Scholar 

  • Symonowicz M, Kolanek M (2012) Flavonoids and their properties to form chelate complexes. Biotechnol Food Sci 76:35–41

    Google Scholar 

  • Teshima K-i, Kaneko T, Ohtani K, Kasai R, Lhieochaiphant S, Picheansoonthon C, Yamasaki K (1997) C-Glycosyl flavones from Clinacanthus nutans (Natural Medicine Note) 生薬學雜誌 51:557

  • Vigneshwaran N, Ashtaputre N, Varadarajan P, Nachane R, Paralikar K, Balasubramanya R (2007) Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater Lett 61:1413–1418

    Article  Google Scholar 

  • Wayne P (2002) National committee for clinical laboratory standards. Perform Stand Antimicrob Disc Suscept Test 12:1–53

    Google Scholar 

Download references

Acknowledgements

Our sincere appreciation to the Universiti Malaysia Pahang for their support and granting research grant RDU170356 to HA Hamid.

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Authors and Affiliations

  1. Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang Kuantan, Pahang, Malaysia

    Hazrulrizawati Abd Hamid & Roziasyahira Mutazah

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  1. Hazrulrizawati Abd Hamid
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  2. Roziasyahira Mutazah
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Correspondence to Hazrulrizawati Abd Hamid.

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Abd Hamid, H., Mutazah, R. Synthesis of Silver Nanoparticles by Clinacanthus nutans Extract Supported with Identification of Flavonoids by UPLC-QTOF/MS and Its Antimicrobial Activity. Iran J Sci Technol Trans Sci 43, 2219–2225 (2019). https://doi.org/10.1007/s40995-018-0659-9

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  • DOI: https://doi.org/10.1007/s40995-018-0659-9

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