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Screening of Medicinal Plants for Potential Source of l-Asparaginase and Optimization of Conditions for Maximum Extraction and Assay of l-Asparaginase from Asparagus racemosus

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Abstract

Different medicinal plants were screened for l-asparaginase. Asparagus racemosus exhibited highest activity (10.11 ± 0.12 U/ml) and specific activity (22.78 ± 0.28 U/mg of protein). A. racemosus was collected from Padmapuram garden of Araku valley, Visakhapatnam district. l-asparaginase activity was detected from the extracts of leaves and rhizome. Enzyme activity was maximum in the extract of rhizome (9.58 ± 0.12 U/ml) with specific activity (21.73 ± 0.28 U/mg of protein) compared with leaves which have the enzyme activity (1.85 ± 0.06 U/ml) with specific activity (2.68 ± 0.14 U/mg of protein). Optimum conditions for the extraction and assay of l-asparaginase from plant rhizomes were studied. Results showed maximum activity of l-asparaginase when the sample to buffer ratio was 3:6, sample weight (g)/buffer volume (ml) using 0.02 M of potassium phosphate buffer solution pH 8.2 at 40 °C. The l-asparaginase activity was 16.01 ± 0.06 U/ml with specific activity (26.81 ± 0.42 U/mg of protein) under optimum conditions.

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  1. Department of Biochemistry, Andhra university, Visakhapatnam, Andhra Pradesh, 530003, India

    K. Beulah & K. P. J. Hemalatha

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  1. K. Beulah
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  2. K. P. J. Hemalatha
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Correspondence to K. Beulah.

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Beulah, K., Hemalatha, K.P.J. Screening of Medicinal Plants for Potential Source of l-Asparaginase and Optimization of Conditions for Maximum Extraction and Assay of l-Asparaginase from Asparagus racemosus. Iran J Sci Technol Trans Sci 43, 1–6 (2019). https://doi.org/10.1007/s40995-017-0359-x

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  • DOI: https://doi.org/10.1007/s40995-017-0359-x

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