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Energy, Ecology and Environment

, Volume 3, Issue 4, pp 216–228 | Cite as

Ionic-liquid-mediated pretreatment and enzymatic saccharification of Prosopis sp. biomass in a consolidated bioprocess for potential bioethanol fuel production

  • Surbhi Vaid
  • Tarun Mishra
  • Bijender Kumar Bajaj
Original Article

Abstract

The efficacy of ionic liquid (IL)-based pretreatment of lignocellulosic biomass (LB) can be enhanced by simultaneous application of surfactants/salts/deep eutectic solvent (DES) systems which may realize more effectual biorefining of LB to biofuels or other commodities. However, due to inhibitory nature of IL, IL-stable saccharification enzymes (cellulase/xylanase) are desired for enzymatic hydrolysis of IL-pretreated biomass. Bacillus spp. are considered as the super microbial factories for production of commercially important robust enzymes. The current study presents the enhanced production (1.438-fold) of an IL-stable cellulase from a newly isolated IL-tolerant Bacillus amyloliquefaciens SV29 by statistical optimization using agroindustrial residues as carbon (groundnut shell) and nitrogen source (mustard cake). The process variables such as groundnut shell and mustard cake, incubation time, and inoculum size were optimized. The enzyme preparation (cellulase/xylanase) was evaluated for its saccharification potential of Prosopis sp. (twigs/pods) biomass that was pretreated either with IL (1-ethyl-3-methylimidazolium methanesulfonate, EMIMS) standalone or IL along with surfactants/salts/DES systems in a consolidated bioprocess (CBP), i.e., one pot consolidated bioprocess, due to several technoeconomic advantages of the latter. No reported studies are available on bioconversion of Prosopis sp. biomass using IL or CBP. Sugar yield was enhanced when IL was used along with either DES choline chloride glycerol (54.4%) or with FeSO4 (51%). Thus, the pretreatment efficacy of EMIMS is substantially enhanced when used in combination with choline chloride glycerol or FeSO4 for getting increased sugar yield upon enzymatic hydrolysis of Prosopis sp. biomass with IL-stable enzymes.

Keywords

Ionic-liquid-stable cellulase Prosopis sp. Pretreatment Surfactant One pot consolidated bioprocess 

Notes

Acknowledgements

Dr. Bijender Kumar (Bajaj) gratefully acknowledges the Institute of Advanced Study, Durham University, UK, for providing COFUND International Senior Research Fellowship for “Research Stay” at Department of Biosciences, Durham University, Durham, UK; Department of Science and Technology (Govt. of India) is acknowledged for financial support (Research Project Ref. SR/SO/BB-66/2007), and Commonwealth Scholarship Commission, UK, for providing Commonwealth Fellowship (INCF-2013-45) for “Research Stay” at Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, UK. Authors thank the Director, School of Biotechnology, University of Jammu, Jammu, for necessary laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Amdebrhan BT, Asfaw S, Assefa G (2016) Acid hydrolysis optimization of Prosopis Juliflora stem for bioethanol production. Science 4:1–11CrossRefGoogle Scholar
  2. Brethauer S, Studer MH (2014) Consolidated bioprocessing of lignocellulose by a microbial consortium. Energy Environ Sci 7:1446–1453CrossRefGoogle Scholar
  3. Chang KL, Chen XM, Han YJ, Wang XQ, Potprommanee L, Ning XA, Liu JY, Sun J, Peng YP, Sun SY, Lin YC (2016) Synergistic effects of surfactant-assisted ionic liquid pretreatment rice straw. Bioresour Technol 214:371–375CrossRefGoogle Scholar
  4. Chang KL, Chen XM, Wang XQ, Han YJ, Potprommanee L, Liu JY, Liao YL, Ning XA, Sun SY, Huang Q (2017) Impact of surfactant type for ionic liquid pretreatment on enhancing delignification of rice straw. Bioresour Technol 227:388–392CrossRefGoogle Scholar
  5. Chen H, Fu X (2016) Industrial technologies for bioethanol production from lignocellulosic biomass. Renew Sust Energ Rev 57:468–478CrossRefGoogle Scholar
  6. Chen S, Zhang X, Ling Z, Xu F (2017) Characterization of the Micromorphology and topochemistry of poplar wood during mild ionic liquid pretreatment for improving enzymatic saccharification. Molecules 22:115CrossRefGoogle Scholar
  7. den Haan R, van Rensburg E, Rose SH, Görgens JF, van Zyl WH (2015) Progress and challenges in the engineering of non-cellulolytic microorganisms for consolidated bioprocessing. Curr Opin Biotechnol 33:32–38CrossRefGoogle Scholar
  8. Fang C, Thomsen MH, Frankær CG, Brudecki GP, Schmidt JE, AlNashef IM (2017) Reviving pretreatment effectiveness of deep eutectic solvents on lignocellulosic date palm residues by prior recalcitrance reduction. Ind Eng Chem Res 56:3167–3174CrossRefGoogle Scholar
  9. Favaro L, Viktor MJ, Rose SH, Viljoen-Bloom M, van Zyl WH, Basaglia M, Cagnin L, Casella S (2015) Consolidated bioprocessing of starchy substrates into ethanol by industrial Saccharomyces cerevisiae strains secreting fungal amylases. Biotechnol Bioeng 112:1751–1760CrossRefGoogle Scholar
  10. Gupta A, Verma JP (2015) Sustainable bio-ethanol production from agro-residues: a review. Renew Sust Energ Rev 41:550–567CrossRefGoogle Scholar
  11. Gupta R, Sharma KK, Kuhad RC (2009) Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. Bioresour Technol 100:1214–1220CrossRefGoogle Scholar
  12. Hou XD, Feng GJ, Ye M, Huang CM, Zhang Y (2017) Significantly enhanced enzymatic hydrolysis of rice straw via a high-performance two-stage deep eutectic solvents synergistic pretreatment. Bioresour Technol 238:139–146CrossRefGoogle Scholar
  13. Irfan M, Mushtaq Q, Tabssum F, Shakir HA, Qazi JI (2017) Carboxymethyl cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology. AMB Express 7:29CrossRefGoogle Scholar
  14. Khudyakov JI, D’haeseleer P, Borglin SE, DeAngelis KM, Woo H, Lindquist EA, Hazen TC, Simmons BA, Thelen MP (2012) Global transcriptome response to ionic liquid by a tropical rain forest soil bacterium, Enterobacter lignolyticus. Proc Natl Acad Sci 109:E2173–E2182CrossRefGoogle Scholar
  15. Kumar R, Tabatabaei M, Karimi K, Sárvári Horváth I (2016) Recent updates on lignocellulosic biomass derived ethanol—a review. Biofuel Res J 9:347–356CrossRefGoogle Scholar
  16. Kurata A, Hirose Y, Misawa N, Hurunaka K, Kishimoto N (2014) Draft genome sequence of the ionic liquid-tolerant bacterium Bacillus amyloliquefaciens CMW1. Genome Announc 2:e01051-14CrossRefGoogle Scholar
  17. Liu Z, Li L, Liu C, Xu A (2018) Pretreatment of corn straw using the alkaline solution of ionic liquids. Bioresour Technol 260:417–420CrossRefGoogle Scholar
  18. Lynd LR, Guss AM, Himmel ME, Beri D, Herring C, Holwerda EK, Murphy SJ, Olson DG, Paye J, Rydzak T, Shao X (2016) Advances in consolidated bioprocessing using Clostridium thermocellum and Thermoanaerobacter saccharolyticum. In: Wittmann C, Liao JC (eds) Industrial biotechnology: microorganisms. Wiley, Weinheim, pp 365–394CrossRefGoogle Scholar
  19. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  20. Mussatto SI (2016) Biomass fractionation technologies for a lignocellulosic feedstock based biorefinery. Elsevier, Amsterdam.  https://doi.org/10.1016/b978-0-12-802323-5.01001-x Google Scholar
  21. Nargotra P, Vaid S, Bajaj BK (2016) Cellulase production from Bacillus subtilis SV1 and its application potential for saccharification of ionic liquid pretreated pine needle biomass under one pot consolidated bioprocess. Fermentation 2:19CrossRefGoogle Scholar
  22. Naseeruddin S, Desai S, Rao LV (2017) Ethanol production from lignocellulosic substrate Prosopis juliflora. Renew Energy 103:701–707CrossRefGoogle Scholar
  23. Nasirpour N, Mousavi SM, Shojaosadati SA (2014) A novel surfactant-assisted ionic liquid pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis. Bioresour Technol 169:33–37CrossRefGoogle Scholar
  24. Nizamudeen S, Bajaj BK (2009) A novel thermo-alkalitolerant endoglucanase production using cost-effective agricultural residues as substrates by a newly isolated Bacillus sp. NZ. Food Technol Biotechnol 47:435–440Google Scholar
  25. Pandey AK, Negi S (2015) Impact of surfactant assisted acid and alkali pretreatment on lignocellulosic structure of pine foliage and optimization of its saccharification parameters using response surface methodology. Bioresour Technol 192:115–125CrossRefGoogle Scholar
  26. Pérez-Pimienta JA, Vargas-Tah A, López-Ortega KM, Medina-López YN, Mendoza-Pérez JA, Avila S, Singh S, Simmons BA, Loaces I, Martinez A (2017) Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production. Bioresour Technol 225:191–198CrossRefGoogle Scholar
  27. Premalatha N, Gopal NO, Jose PA, Anandham R, Kwon SW (2015) Optimization of cellulase production by Enhydrobacter sp. ACCA2 and its application in biomass saccharification. Front Microbiol 6:1046CrossRefGoogle Scholar
  28. Procentese A, Johnson E, Orr V, Campanile AG, Wood JA, Marzocchella A, Rehmann L (2015) Deep eutectic solvent pretreatment and subsequent saccharification of corncob. Bioresour Technol 192:31–36CrossRefGoogle Scholar
  29. Sharma M, Bajaj BK (2014) Cellulase production from Bacillus subtilis MS 54 and its potential for saccharification of biphasic-acid-pretreated rice straw. J Biobased Mater Bioenergy 8:449–456CrossRefGoogle Scholar
  30. Singh S, Bajaj BK (2017) Agroindustrial/forestry residues as substrates for production of thermoactive alkaline protease from Bacillus licheniformis K-3 having multifaceted hydrolytic potential. Waste Biomass Valor 8:453–462CrossRefGoogle Scholar
  31. Singh S, Sambhyal M, Vaid S, Singh P, Bajaj BK (2015) Two-step sequential optimization for production of ionic liquid stable cellulase from Bacillus subtilis I-2. Biocatal Biotransfor 33:224–233CrossRefGoogle Scholar
  32. Trivedi N, Gupta V, Reddy CRK, Jha B (2013) Detection of ionic liquid stable cellulase produced by the marine bacterium Pseudoalteromonas sp. isolated from brown alga Sargassum polycystum C. Agardh. Bioresour Technol 132:313–319CrossRefGoogle Scholar
  33. Vaid S, Bajaj BK (2017) Production of ionic liquid tolerant cellulase from Bacillus subtilis G2 using agroindustrial residues with application potential for saccharification of biomass under one pot consolidated bioprocess. Waste Biomass Valor 8:949–964CrossRefGoogle Scholar
  34. Vaid S, Nargotra P, Bajaj BK (2017) Consolidated bioprocessing for biofuel-ethanol production from pine needle biomass. Environ Prog Sustain Energy 37:546–552CrossRefGoogle Scholar
  35. Vaid S, Bhat N, Nargotra P, Bajaj BK (2018) Combinatorial application of ammonium carbonate and sulphuric acid pretreatment to achieve enhanced sugar yield from pine needle biomass for potential biofuel–ethanol production. Energ Ecol Environ 3:126–135CrossRefGoogle Scholar
  36. Vogel KP, Dien BS, Jung HG, Casler MD, Masterson SD, Mitchell RB (2011) Quantifying actual and theoretical ethanol yields for switchgrass strains using NIRS analyses. Bioenergy Res 4:96–110CrossRefGoogle Scholar
  37. Xu J, He B, Wu B, Wang B, Wang C, Hu L (2014) An ionic liquid tolerant cellulase derived from chemically polluted microhabitats and its application in in situ saccharification of rice straw. Bioresour Technol 157:166–173CrossRefGoogle Scholar
  38. Xu J, Wang X, Liu X, Xia J, Zhang T, Xiong P (2016) Enzymatic in situ saccharification of lignocellulosic biomass in ionic liquids using an ionic liquid-tolerant cellulases. Biomass Bioenergy 93:180–186CrossRefGoogle Scholar
  39. Yu C, Simmons BA, Singer SW, Thelen MP, VanderGheynst JS (2016) Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts. Appl Microbial Biotechnol 100:10237–10249CrossRefGoogle Scholar
  40. Yuan X, Duan Y, He L, Singh S, Simmons B, Cheng G (2017) Characterization of white poplar and eucalyptus after ionic liquid pretreatment as a function of biomass loading using X-ray diffraction and small angle neutron scattering. Bioresour Technol 232:113–118CrossRefGoogle Scholar
  41. Zabed H, Sahu JN, Boyce AN, Faruq G (2016) Fuel ethanol production from lignocellulosic biomass: an overview on feedstocks and technological approaches. Renew Sust Energ Rev 66:751–774CrossRefGoogle Scholar
  42. Zhang CW, Xia SQ, Ma PS (2016) Facile pretreatment of lignocellulosic biomass using deep eutectic solvents. Bioresour Technol 219:1–5CrossRefGoogle Scholar
  43. Zhao J, Zhang H, Zheng R, Lin Z, Huang H (2011) The enhancement of pretreatment and enzymatic hydrolysis of corn stover by FeSO4 pretreatment. Biochem Eng J 56:158–164CrossRefGoogle Scholar

Copyright information

© Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Surbhi Vaid
    • 1
  • Tarun Mishra
    • 1
  • Bijender Kumar Bajaj
    • 1
  1. 1.School of BiotechnologyUniversity of JammuJammuIndia

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