Proteomic Analysis of Low-Grade Serous Ovarian Cancer and Comparing It with Non-cancerous Ovarian

  • Haniyeh Bashizadeh Fakhar
  • Mostafa Rezaie-Tavirani
  • Hakimeh Zali
  • Roya Faraji Darkhaneh
  • Ehsan Kazem Nejad
  • Mohammad Hossein Aghazadeh
Original Article



In Iran, ovarian cancer is the eighth most common cancer. Serous is considered as the most common of all ovarian cancers. It is usually deadly due to late diagnosis. Moreover, as all studies so far have considered metastatic or high-stage ovarian cancer, this study sought low-grade ovarian serous in human, which is of high importance and aimed at obtaining protein biomarkers that may be useful in diagnosis and treatment of ovarian serous cancer.

Materials and Methods

In this experimental study, ten patients with proved low-grade serous ovarian cancer and ten women without ovarian cancer, healthy, and cancerous tissue samples were obtained to perform proteomics. All tissue proteins were purified using standard separation methods, and proteins were separated using two-dimensional electrophoresis. Healthy and tumorous tissue proteomes were compared, and the extent of protein expression was analyzed.


This study identified 212 proteins, 41 of which had changes in protein expression in cancer state. The expression of eight proteins was suppressed, and five proteins were expressed in cancerous group. Thirteen proteins in cancer sample showed increase in expression and 15 proteins reduced expression in cancerous tissue.


It seems that in this disease, the expression of a large number of proteins in cancerous tissues changed, most of them decreased or not expressed at all. These changed proteins can be effective as biomarker in diagnosis and treatment of this disease.


Proteomics Low-grade serous ovarian cancer Comparison Non-cancerous ovarian 


Authors’ contributions

All authors had equal role in design, work, statistical analysis, and manuscript writing.


This study was funded by Guilan University of Medical Sciences.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and Animal Rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Also ethics committee of Shahid Beheshti University of Medical Sciences approved this study by IR.SBMU.RETECH.REC.1396.709.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Association of Gynecologic Oncologists of India 2018

Authors and Affiliations

  • Haniyeh Bashizadeh Fakhar
    • 1
  • Mostafa Rezaie-Tavirani
    • 1
  • Hakimeh Zali
    • 2
  • Roya Faraji Darkhaneh
    • 3
  • Ehsan Kazem Nejad
    • 4
  • Mohammad Hossein Aghazadeh
    • 5
  1. 1.Proteomics Research CenterShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Proteomics Research Center, School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Department of Obstetrics and Gynecology, Reproductive Health Research CenterGuilan University of Medical SciencesRashtIran
  4. 4.Department of Biostatistics, Guilan Road Truma Research CenterGuilan University of Medical SciencesRashtIran
  5. 5.Azad Medical UniversityTehranIran

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