Development of Porous Hydrogel Scaffolds with Multiple Cues for Liver Tissue Engineering
Development of tissue-engineered construct for hepatic regeneration remains challenging due to the lack of potential milieu to control the trans-differentiation of hepatocytes. In the present study, galactose containing poly(vinyl alcohol) (PVA) and gelatin (8:2 and 9:1) hydrogels impregnated with hepatocyte growth factor (HGF)-loaded nanoparticles (241 ± 56 nm) using freeze/thaw technique was fabricated. The scaffolds with the HGF encapsulation efficiency of 57 ± 2.9% exhibited two-stage release kinetics for 30 days. Hepatogenic potential of the fabricated scaffolds were evaluated by determining the adhesion, viability, and proliferation with functional activity of rat primary hepatocytes for 28 days. Scaffolds of 8:2 ratios of PVA and gelatin maintained spheroidal morphology of hepatocytes and exhibited significantly higher metabolic secretions for 28 days (p < 0.05). Hence, galactose-based PVA-gelatin (8:2) hydrogel scaffold could be a promising alternative substrate to retain the phenotype and functional characteristics of hepatocytes due to the co-existence of bioactive motif and trophic factors.
The developed 3D scaffold with growth factors encapsulated nanospheres possesses characteristic features for hepatic regeneration and is proposed to be a candidate material for further invivo evaluation
KeywordsHydrogel Hepatocyte growth factor Nanospheres Poly(vinyl alcohol) Poly(lactide-co-glycolide) primary hepatocytes
The authors wish to acknowledge the Indian Council for Medical Research (35/22/2010-BMS), Nano Mission (SR/S5/NM–07/2006 and SR/NM/PG–16/2007), FIST program—Department of Science & Technology, Govt. of India for funding (SR/FST/LSI–327/2007), and FIST pharmacy program (SR/FST/LSI-058/2010) for cell culture facility. Prof. T. R. Rajagopalan R & D Cell of SASTRA University is also acknowledged.
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