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Interaction of a tomato leaf curl New Delhi virus with a betasatellite enhances symptom severity in field-infected tomato plants

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Abstract

Two field-collected tomato plants, with severe and mild tomato leaf curl disease symptoms, were shown to be infected with tomato leaf curl New Delhi virus. The plant with severe symptoms was shown to additionally contain tobacco leaf curl betasatellite (TobLCuB). Inoculation of Nicotiana benthamiana plants with the cloned components showed the severe symptoms to be due to the presence of TobLCuB. A shorter latent period was associated with the presence of TobLCuB except in the presence of the DNA-A and DNA-B components of the severe type. The DNA-B component from the mild type also reduced the latent period, more so than the DNA-B from the severe type, except in the interaction with the DNA-A from the mild type. These differences in the effects of the virus components from the two isolates may possibly be due to mutations in the DNA-B from the severe type. The results show that betasatellites can enhance the virulence of bipartite begomoviruses, even for isolates that induce quite mild symptoms.

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All data generated or analyzed during this study are included in this published article.

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Acknowledgments

MSS was supported by a Ph.D. fellowship and International Research Support Initiative Program (IRSIP) fellowship from the Higher Education Commission (HEC), Government of Pakistan. RWB was supported by the HEC under the ‘Foreign Faculty Hiring Program’.

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Author notes

  1. M. S. Shahid

    Present address: Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod, 123, Muscat, Oman

Authors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad, Pakistan

    M. S. Shahid, S. Mansoor & R. W. Briddon

  2. School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA

    M. S. Shahid & J. K. Brown

Authors
  1. M. S. Shahid
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  2. S. Mansoor
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  4. R. W. Briddon
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Contributions

MS, SM, and RWB conceived and designed the study. MS conducted the study and analyzed the results with the assistance of RWB and JB. The first draft of the manuscript was written by MS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to M. S. Shahid.

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There is no involvement of any human or animals by any of the authors in this study.

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Shahid, M.S., Mansoor, S., Brown, J.K. et al. Interaction of a tomato leaf curl New Delhi virus with a betasatellite enhances symptom severity in field-infected tomato plants. Trop. plant pathol. 46, 169–174 (2021). https://doi.org/10.1007/s40858-020-00414-0

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