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Assessment of antimicrobial potential of iron on phytopathogenic isolates of Nocardia sp. and their effect on tobacco false broomrape symptom expression

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Abstract

Tobacco crop is affected by false broomrape caused by phytopathogenic isolates belonging to the Nocardia genus, present in the soil. Both persistence and incidence levels of its causal agent are lower in soils with red to reddish tonalities. These tones are associated with the presence of oxidized iron forms. The aim of this research was to assess the antimicrobial potential of different chemical forms of iron on phytopathogenic isolates of Nocardia sp., and evaluate its effect on the symptom expression of false broomrape in tobacco plants. Susceptibility to iron salts of four isolates was assessed in vitro by the minimal inhibitory concentration test. In in vivo experiments, soil inoculated with phytopathogenic isolates was treated with FeSO4.7H2O and symptom expression of false broomrape was evaluated as the average degree of affection, which considers grades of growth of tumours and buds developed on roots. We found that phytopathogenic isolates from four tobacco areas of Cuba with high incidence of the disease showed in vitro susceptibility to free iron in its oxidized forms of Fe+2 and Fe+3. It was shown that the application of 1000 ppm of FeSO4.7H2O on the roots of tobacco seedlings and in the soil where they were transplanted reduces expression of the symptoms of false broomrape.

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Acknowledgements

Our thanks to Dr. Nóra Mendler-Drienyovszki (Research Institute of Nyíregyháza, University of Debrecen, Hungary) and to Dr. Vivian Marlene Izaguirre-García (University of San Carlos, Guatemala) for their special contribution as critical reviewers of the manuscript.

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Authors and Affiliations

  1. Instituto de Investigaciones del Tabaco, Carretera a Tumbadero km 8.5, San Antonio de los Baños, 32500, Artemisa, Cuba

    Yunior Miguel Morán-Gómez, Abdón Joaquín Trémols-González & Rosario Domínguez-Larrinaga

  2. Facultad de Ingeniería y Ciencias Empresariales, Universidad de Artemisa, Calle 8C No. 713. e/ 7 y Campo, Manzana 22, Zona Urbana Consejo Popular Lincoln, 33800, Artemisa, Cuba

    Yunior Miguel Morán-Gómez

  3. Colegio de Postgraduados, Campus Montecillo, Carretera México-Texcoco km 36.5, Montecillo, Texcoco, 56230, México

    María Guadalupe Carrillo-Benites

  4. UCTB Estación Experimental San Juan y Martínez, Instituto de Investigaciones del Tabaco, Carretera Panamericana km 62.5, Finca Vivero, San Juan y Martínez, 23200, Pinar del Río, Cuba

    José Ramón Cabrera-Alfonso

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  1. Yunior Miguel Morán-Gómez
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  2. Abdón Joaquín Trémols-González
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  3. Rosario Domínguez-Larrinaga
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  4. María Guadalupe Carrillo-Benites
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  5. José Ramón Cabrera-Alfonso
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Correspondence to Yunior Miguel Morán-Gómez.

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Section Editor: Marcelo Carmona

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Morán-Gómez, Y.M., Trémols-González, A.J., Domínguez-Larrinaga, R. et al. Assessment of antimicrobial potential of iron on phytopathogenic isolates of Nocardia sp. and their effect on tobacco false broomrape symptom expression. Trop. plant pathol. 43, 333–340 (2018). https://doi.org/10.1007/s40858-018-0213-5

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  • DOI: https://doi.org/10.1007/s40858-018-0213-5

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