Skip to main content
SpringerLink
Log in

Multi-host species of Cercospora are associated with Cercospora leaf blight and purple seed stain of soybean

  • Short Communication
  • Published:
Tropical Plant Pathology Aims and scope Submit manuscript

Abstract

Many Cercospora spp. are multi-host pathogens. The versatility of Cercospora newly associated with Cercospora leaf blight (CLB) and purple seed stain (PSS) in soybean seems to have been historically underestimated. Herein, we assembled multilocus sequence data (seven nuclear gene regions) with sequences from 36 fungal isolates sampled from non-soybean host species from Brazil (15 species) and United States (six species), in addition to 13 isolates from soybean. Phylogenetic tools explored relationships among isolates of Cercospora obtained from either soybeans or non-soybean hosts. Cercosporin production was determined to confirm whether the isolates belong to cercosporin-producing species of Cercospora. Among the five genealogical lineages, Lineage 1 showed C. kikuchii as the only member; this species is known to infect only soybean. The remaining four lineages were multi-host Cercospora which infected soybean along with ornamental and weed species. Genealogical placements were unrelated to host association and isolates clustered according to geography. Multi-host species of cercosporin-producing Cercospora are associated with CLB and PSS of soybean.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

References

  • Albu S, Schneider RW, Price PP, Doyle VP (2016) Cercospora Cf. flagellaris and C. Cf. sigesbeckiae are associated with Cercospora leaf blight and purple seed stain on soybean in North America. Phytopathology 106:1376–1385.

    Article  CAS  PubMed  Google Scholar 

  • Alfenas AC, Ferreira FA, Mafia RG, Gonçalves RC (2016) Isolamento de fungos fitopatogênicos. In: Alfenas AC, Mafia RG (eds) Métodos em fitopatologia, 2nd edn. Viçosa, Brazil. Editora UFV, pp 55–91

    Google Scholar 

  • Almeida AMR, Piuga FF, Marin SRR, Binneck E, Sartori F, Costamilan LM, Teixeira MRO, Lopes M (2005) Pathogenicity, molecular characterization, and cercosporin content of Brazilian isolates of Cercospora kikuchii. Fitopatologia Brasileira 30:594–602.

    Article  Google Scholar 

  • Cai L, Giraud T, Zhang N, Begerow D, Cai G, Shivas RG (2011) The evolution of species concepts and species recognition criteria in plant pathogenic fungi. Fungal Diversity 50:121–133.

    Article  Google Scholar 

  • Chupp C (1954) A monograph of the fungus genus Cercospora. The Ronald Press, Ithaca

    Google Scholar 

  • Cooperman CJ, Jenkins SF (1986) Conditions influencing growth and sporulation of Cercospora asparagi and Cercospora blight development in asparagus. Phytopathology 76:617–622.

    Article  Google Scholar 

  • Crous PW, Braun U (2003) Mycosphaerella and its anamorphs: 1. Names published in Cercospora and Passalora. Utrecht, The Netherlands: Centraalbureau voor Schimmelcultures (CBS)

  • Crous PW, Groenewald JZ (2005) Hosts, species and genotypes: opinions versus data. Australasian Plant Pathology 34:463–470.

    Article  Google Scholar 

  • Daub ME, Herrero S, Chung KR (2005) Photoactivated perylenequinone toxins in fungal pathogenesis of plants. FEMS Microbiology Letters 252:197–206.

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Fajola AO (1978) Cercosporin, a phytotoxin from Cercospora spp. Physiology. Plant Pathology 13:157–164

    CAS  Google Scholar 

  • Fresenius G (1863) Beiträge zur Ökologie 3. Frankfurt, German: Heinrich Ludwig Brommer Verlag

  • Goodwin SB, Dunkle LD, Zismann VL (2001) Phylogenetic analysis of Cercospora and Mycosphaerella based on the internal transcribed spacer region of ribosomal DNA. Phytopathology 91:648–658.

    Article  CAS  PubMed  Google Scholar 

  • Groenewald M, Groenewald JZ, Braun U, Crous PW (2006) Host range of Cercospora apii and C. beticola and description of C. apiicola, a novel species from celery. Mycologia 98:275–285.

    Article  PubMed  Google Scholar 

  • Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U, Crous PW (2012) Species concepts in Cercospora: spotting the weeds among the roses. Studies in Mycology 75:115–170

    Article  PubMed Central  Google Scholar 

  • Jenns AE, Daub ME, Upchurch RG (1989) Regulation of cercosporin accumulation in culture by medium and temperature manipulation. Phytopathology 79:213–219.

    Article  CAS  Google Scholar 

  • Matsumoto T, Tomoyasu R (1925) Studies on the purple speck of soybean seed. Annals of the Phytopathological Society of Japan 1:1–14.

    Article  Google Scholar 

  • Paul PA, Munkvold GP (2005) Influence of temperature and relative humidity on sporulation of Cercospora zeae-maydis and expansion of gray leaf spot lesions on maize leaves. Plant Disease 89:624–630.

    Article  Google Scholar 

  • Pollack FG (1987) An annotated compilation of Cercospora names. Mycologia Memoir 12:1–212

    Google Scholar 

  • R Core Team (2017) R: a language and environment for statistical computing. R foundation for statistical computing. Available at: https://www.R-project.org/

  • Satterthwaite FE (1946) An approximate distribution of estimates of variance components. Biometrics Bulletin 2:110–114.

    Article  CAS  PubMed  Google Scholar 

  • Soares APG, Guillin EA, Borges LL, Silva ACT, Almeida AMR, Grijalba PE, Gottlieb AM, Bluhm BH, Oliveira LO (2015) More Cercospora species infect soybeans across the americas than meets the eye. PLoS One 10:e0133495.

    Article  PubMed  PubMed Central  Google Scholar 

  • Upchurch RG, Ehrenshaft M, Walker DC, Sanders LA (1991) Genetic-transformation system for the fungal soybean pathogen Cercospora kikuchii. Applied Environmental Microbiology 57:2935–2939

    CAS  PubMed  PubMed Central  Google Scholar 

  • Walters HJ (1980) Soybean leaf blight caused by Cercospora kikuchii. Plant Disease 64:961–962.

    Article  Google Scholar 

Download references

Acknowledgements

We are grateful to those who provided assistance collecting samples. LLB received a scholarship from National Council of Scientific and Technological Development (CNPq; 140428/2014-3) and CAPES Foundation (8781/14-1). LOO received fellowships from CNPq (PQ 305827/2015-4, PDE 200786/2015-6) and grants from the Minas Gerais State Foundation of Research Aid (FAPEMIG; PPM 00561-15), CNPq (478752/2013–0), and CAPES (PPCP–Mercosul 015/2011). TFF received a fellowship from CAPES (PNPD 2010). MGL received a fellowship from CNPq (IC 104284/2016-1). IDC received a fellowship from CNPq (IC 121557/2014-6). BHB was supported by award #1253256 from the U.S. National Science Foundation. The National Institute of Food and Agriculture, U.S. Department of Agriculture (award #1008895 to BHB) provided additional support.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil

    L. L. Borges, T. F. Ferreira, M. G. Lana, I. D. Caliman & L. O. Oliveira

  2. University of Arkansas, Fayetteville, AR, 72701, USA

    B. H. Bluhm

Authors
  1. L. L. Borges
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. F. Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. G. Lana
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. D. Caliman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. H. Bluhm
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. O. Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. O. Oliveira.

Additional information

Senior Editor: Sarah J. Pethybridge

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Borges, L.L., Ferreira, T.F., Lana, M.G. et al. Multi-host species of Cercospora are associated with Cercospora leaf blight and purple seed stain of soybean. Trop. plant pathol. 43, 170–177 (2018). https://doi.org/10.1007/s40858-017-0205-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40858-017-0205-x

Keywords

Search

Navigation