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Tropical Plant Pathology

, Volume 42, Issue 2, pp 121–125 | Cite as

Antifungal activity of Musa phyllosphere Bacillus pumilus strain against Mycosphaerella fijiensis

  • Mileidy Cruz-Martín
  • Mayra Acosta-Suárez
  • Eilyn Mena
  • Berkis Roque
  • Tatiana Pichardo
  • Yelenys Alvarado-Capó
Short Communication

Abstract

Black leaf streak disease (BLSD; also known as black Sigatoka) caused by Mycosphaerella fijiensis Morelet is one of the main diseases affecting banana trees worldwide. Associated microorganisms in the phylloplane have been previously studied for their control. An exhaustive understanding of biocontrol mechanisms will potentiate the biological control agent. Therefore, strain Bacillus pumilus CCIBP-C5, isolated from banana leaves surface was evaluated for in vitro antagonism against M. fijiensis and the effect of culture filtrate on fungal growth and disease progress was determined. Bacterial cell and its diffused metabolites inhibited M. fijiensis growth. The culture filtrate of B. pumilus affected the mycelial growth, with antifungal effect on hyphal tip, membrane permeability and integrity. Also, a reduction of number of BLSD necrotic lesions and disease progression were found after application of the B. pumilus CCIBP-5 culture filtrate in banana plants. Results indicate that CCIBP-C5 has potential for biological control of BLSD.

Keywords

Black Sigatoka Biological control Culture filtrate Greenhouse Mycelial damage 

Notes

Acknowledgements

The authors are grateful to MSc. Miguel Tzec for his help in microscopy technics. Partial financial support for this work was an output of a scholarship from the Food Security Center from the University of Hohenheim, which is part of the DAAD (German Academic Exchange Service) program “exceed” in cooperation with the host institute Centro de Investigación Científica de Yucatán (CICY), México and PhD Blondy Canto Canché.

References

  1. Alvarado-Capó Y, Leiva-Mora M, Rodríguez MA, Acosta-Suárez M, Cruz-Martín M, Portal O, Kosky RG, García L, Bermúdez I, Padrón J (2003) Early evaluation of black leaf streak resistance by using mycelial suspensión of Mycosphaerella fijiensis. In: Jacome L, Lepoivre P, Martin D, Ortiz R, Romero R, Escalante JV (eds) Mycosphaerella leaf spot diseases of bananas: present status and outlook. Proceedings of the Workshop on Mycosphaerella Leaf Spot Diseases, San José, Costa Rica. The International Network for the Improvement of Banana and Plantain, Montpellier, pp 169–175Google Scholar
  2. Ceballos I, Mosquera S, Angulo M, Mira J, Argel L, Uribe-Velez D, Romero-Tabarez M, Orduz-Peralta S, Villegas V (2012) Cultivable bacteria populations associated with leaves of banana and plantain plants and their antagonistic activity against Mycosphaerella fijiensis. Microb Ecol 64:641–653CrossRefPubMedGoogle Scholar
  3. Choi G, Lee HJ, Cho K (1996) Lipid peroxidation and membrane disruption by vinclozolin in dicarboximide-susceptible and resistant isolates of Botrytis cinerea. Pest Biochem Physiol 55:29–39CrossRefGoogle Scholar
  4. Churchill AC (2011) Mycosphaerella fijiensis, the black leaf streak pathogen of banana: progress towards understanding pathogen biology and detection, disease development, and the challenges of control. Mol Plant Pathol 12:307–328CrossRefPubMedGoogle Scholar
  5. Cruz-Martín M, Acosta-Suárez M, Mena E, Roque B, Leiva-Mora M, Pichardo T, del Pilar-Castro R, Alvarado-Capó Y (2013) Cuantificación del crecimiento in vitro de Mycosphaerella fijiensis mediante lecturas de absorbancia. Biotecnología Vegetal 13:219–224Google Scholar
  6. Fu G, Huang S, Ye Y, Wu Y, Cen Z, Lin S (2010) Characterization of a bacterial biocontrol strain B106 and its efficacy in controlling banana leaf spot and post-harvest anthracnose diseases. Biol Control 55:1–10CrossRefGoogle Scholar
  7. Gutierrez-Monsalve JA, Mosquera S, González-Jaramillo LM, Mira JJ, Villegas-Escobar V (2015) Effective control of black Sigatoka disease using a microbial fungicide based on Bacillus subtilis EA-CB0015 culture. Biol Control 87:39–46CrossRefGoogle Scholar
  8. Gutiérrez-Román MI, Holguín-Meléndez F, Dunn F, Guillén K, Huerta-Palacios G (2015) Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella fijiensis, causal agent of black Sigatoka in banana (Musa spp.). BioControl 60:565–572Google Scholar
  9. Johanson LH, Jeger MJ (1993) Use of PCR for detection of Mycosphaerella fijiensis and musicola, the causal agents of Sigatoka leaf spots in banana and plantain. Mycol Res 97:670–674CrossRefGoogle Scholar
  10. Leiva-Mora M, Alvarado-Capó Y, Acosta-Suárez M, Cruz-Martín M, Sánchez-García C, Roque B (2010) Protocolo para la inoculación artificial de plantas de Musa spp. con Mycosphaerella fijiensis y evaluación de su respuesta mediante variables epifitiológicas y componentes de la resistencia. Biotecnología Vegetal 10:79–88Google Scholar
  11. Matteuci E, Biasci E, Giampietro O (2001) Advanced oxidation protein products in plasma: stability during storage and corrrelation with other clinical characteristics. Acta Diabetol 38:187–189CrossRefGoogle Scholar
  12. Moreno B, Dunn F, Guillén K, Holguín F, Hernández M, Encarnación S, Huerta G (2016) Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella fijiensis Morelet. World J Microbiol Biotechnol 32:44CrossRefGoogle Scholar
  13. Ongena M, Jourdan E, Adam A, Paquot M, Brans A, Joris B (2007) Surfactin and fengycin lipopeptidesof Bacillus subtilis as elicitors of induced systemic resistance in plants. Environ Microbiol 9:1084–1090CrossRefPubMedGoogle Scholar
  14. Ruangwong O, Chang C, Lamine S, Liang W (2012) Identification of antifungal compound produced by Bacillus subtilis LB5 with ability to control anthracnose disease caused by Colletotrichum gloeosporioides. Afr J Microbiol Res 6:3732–3738Google Scholar
  15. Shanner G, Finney RE (1977) The effect of nitrogen fertilization on the expression of show-mildewing resistance in Knox wheat. Phytopathology 67:1051–1056CrossRefGoogle Scholar
  16. Stein T (2005) Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 56:845–857CrossRefPubMedGoogle Scholar
  17. Wang C, Xing J, Chin CK, Peters JS (2002) Fatty acids with certain structural characteristics are potent inhibitors of germination and inducers of cell death of powdery mildew. Physiol Mol Plant Pathol 61:151–161CrossRefGoogle Scholar
  18. Zhou W, Huang J, Niu T (2008) Isolation of an antifungal Paenibacillus strain HT16 from locusts and purification of its medium dependent antagonistic component. J Appl Microbiol 105:912–919CrossRefPubMedGoogle Scholar

Copyright information

© Sociedade Brasileira de Fitopatologia 2017

Authors and Affiliations

  • Mileidy Cruz-Martín
    • 1
  • Mayra Acosta-Suárez
    • 1
  • Eilyn Mena
    • 1
  • Berkis Roque
    • 1
  • Tatiana Pichardo
    • 1
  • Yelenys Alvarado-Capó
    • 1
  1. 1.Instituto de Biotecnología de las PlantasUniversidad Central “Marta Abreu” de Las VillasSanta ClaraCuba

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