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Antifungal activity of Musa phyllosphere Bacillus pumilus strain against Mycosphaerella fijiensis

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Abstract

Black leaf streak disease (BLSD; also known as black Sigatoka) caused by Mycosphaerella fijiensis Morelet is one of the main diseases affecting banana trees worldwide. Associated microorganisms in the phylloplane have been previously studied for their control. An exhaustive understanding of biocontrol mechanisms will potentiate the biological control agent. Therefore, strain Bacillus pumilus CCIBP-C5, isolated from banana leaves surface was evaluated for in vitro antagonism against M. fijiensis and the effect of culture filtrate on fungal growth and disease progress was determined. Bacterial cell and its diffused metabolites inhibited M. fijiensis growth. The culture filtrate of B. pumilus affected the mycelial growth, with antifungal effect on hyphal tip, membrane permeability and integrity. Also, a reduction of number of BLSD necrotic lesions and disease progression were found after application of the B. pumilus CCIBP-5 culture filtrate in banana plants. Results indicate that CCIBP-C5 has potential for biological control of BLSD.

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Acknowledgements

The authors are grateful to MSc. Miguel Tzec for his help in microscopy technics. Partial financial support for this work was an output of a scholarship from the Food Security Center from the University of Hohenheim, which is part of the DAAD (German Academic Exchange Service) program “exceed” in cooperation with the host institute Centro de Investigación Científica de Yucatán (CICY), México and PhD Blondy Canto Canché.

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Authors and Affiliations

  1. Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní km 5.5, Santa Clara, Villa Clara, Cuba, 54830

    Mileidy Cruz-Martín, Mayra Acosta-Suárez, Eilyn Mena, Berkis Roque, Tatiana Pichardo & Yelenys Alvarado-Capó

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  1. Mileidy Cruz-Martín
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  2. Mayra Acosta-Suárez
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  3. Eilyn Mena
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  4. Berkis Roque
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  5. Tatiana Pichardo
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  6. Yelenys Alvarado-Capó
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Correspondence to Mileidy Cruz-Martín.

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Section Editor: Bernardo A. Halfeld-Vieira

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Cruz-Martín, M., Acosta-Suárez, M., Mena, E. et al. Antifungal activity of Musa phyllosphere Bacillus pumilus strain against Mycosphaerella fijiensis . Trop. plant pathol. 42, 121–125 (2017). https://doi.org/10.1007/s40858-017-0139-3

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  • DOI: https://doi.org/10.1007/s40858-017-0139-3

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