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In vitro and in vivo investigation on biodegradable Mg-Li-Ca alloys for bone implant application

镁锂钙合金作为骨植入材料的体内外研究

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Abstract

Magnesium alloys show promise for application in orthopedic implants, owing to their biodegradability and biocompatibility. In the present study, ternary Mg-(3.5, 6.5 wt%) Li-(0.2, 0.5, 1.0 wt%) Ca alloys were developed. Their mechanical strength, corrosion behavior and cytocompatibility were studied. These alloys showed improved mechanical strength than pure Mg and exhibited suitable corrosion resistance. Furthermore, Mg-3.5Li-0.5Ca alloys with the best in vitro performance were implanted intramedullary into the femurs of mice for 2 and 8 weeks. In vivo results revealed a significant increase in cortical bone thickness around the Mg-3.5Li-0.5Ca alloy rods, without causing any adverse effects. Western blotting and immunofluorescence staining of β-catenin illustrated that Mg-3.5Li-0.5Ca alloy extracts induced osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMMSCs) through the canonical Wnt/β-catenin pathway. Our studies demonstrate that Mg-3.5Li-0.5Ca alloys hold much promise as candidates for the facilitation of bone implant application.

摘要

本文制备了三元Mg-(3.5, 6.5 wt.%)Li-(0.2, 0.5, 1.0 wt.%)Ca合金, 并研究了其力学性能、 腐蚀性能与生物相容性. 此合金的力学性能较纯镁显著提高, 并具有良好的耐腐蚀性. 然后, 将体外性能最佳的Mg-3.5Li-0.5Ca合金植入小鼠股骨骨髓腔, 体内实验结果显示, Mg-3.5Li-0.5Ca合金周围的骨厚度增加, 未见不良反应. Western blot和免疫荧光染色结果显示, Mg-3.5Li-0.5Ca合金通过经典的Wnt/β-catenin信号通路促进了人骨髓间充质干细胞的成骨向分化. 研究结果表明, Mg-3.5Li-0.5Ca合金具有作为骨植入材料的巨大潜力.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2016YFC1102900 and 2016YFC1102402), the National Natural Science Foundation of China (81771039, 81470769 and 51431002), the Project for Culturing Leading Talents in Scientific and Technological Innovation of Beijing, China (Z171100001117169), the NSFC-RFBR Cooperation Project (51611130054), and the NSFC/RGC Joint Research Scheme (51361165101 and 5161101031).

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Authors and Affiliations

  1. Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China

    Dandan Xia  (夏丹丹), Siyi Wang  (王思仪), Yunsong Liu  (刘云松) & Yongsheng Zhou  (周永胜)

  2. Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing, 100871, China

    Yang Liu  (刘洋) & Yufeng Zheng  (郑玉峰)

  3. College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, China

    Rong-Chang Zeng  (曾荣昌)

  4. National Engineering Laboratory for Digital and Material Technology of Stomatology, National Clinical Research Center for Oral Diseases, Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, China

    Yunsong Liu  (刘云松) & Yongsheng Zhou  (周永胜)

Authors
  1. Dandan Xia  (夏丹丹)
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  2. Yang Liu  (刘洋)
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  3. Siyi Wang  (王思仪)
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  4. Rong-Chang Zeng  (曾荣昌)
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  5. Yunsong Liu  (刘云松)
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  6. Yufeng Zheng  (郑玉峰)
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  7. Yongsheng Zhou  (周永胜)
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Corresponding authors

Correspondence to Yunsong Liu  (刘云松) or Yufeng Zheng  (郑玉峰).

Additional information

Dandan Xia is currently a PhD student at the School and Hospital of Stomatology, Peking University. She received her Bachelor’s degree from Peking University in 2015. Her research focuses on the magnesium alloys and mesenchymal stem cells.

Yunsong Liu received his PhD in the School and Hospital of Stomatology from Peking University, China, in 2008. Since 2008, he has been a full professor at Peking University in Beijing, China. His research focuses on the magnesium alloys and mesenchymal stem cells.

Yufeng Zheng received his PhD in materials science from Harbin Institute of Technology, China, in 1998. Since 2004, he has been a full professor at Peking University in Beijing, China. His research focuses on the development of various new biomedical metallic materials (biodegradable Mg, Fe and Zn based alloys, β-Ti alloys with low elastic modulus, bulk metallic glass, ultra-fine grained metallic materials, etc.).

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Xia, D., Liu, Y., Wang, S. et al. In vitro and in vivo investigation on biodegradable Mg-Li-Ca alloys for bone implant application. Sci. China Mater. 62, 256–272 (2019). https://doi.org/10.1007/s40843-018-9293-8

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  • DOI: https://doi.org/10.1007/s40843-018-9293-8

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