Science China Materials

, Volume 60, Issue 11, pp 1129–1144 | Cite as

Synthesis of magnetic core-branched Au shell nanostructures and their application in cancer-related miRNA detection via SERS

  • Yanjun Yang (杨琰君)
  • Xinyu Jiang (蒋新宇)
  • Jie Chao (晁洁)
  • Chunyuan Song (宋春元)
  • Bing Liu (刘冰)
  • Dan Zhu (朱丹)
  • Youzhi Sun (孙友志)
  • Boyue Yang (杨博玥)
  • Qiaowei Zhang (张乔伟)
  • Ye Chen (陈叶)
  • Lianhui Wang (汪联辉)
Articles

Abstract

Magnetic core gold shell nanostructures which integrate both SERS activity and superparamagnetism are widely utilized in SERS-based detection as SERS substrates, sample separation and preconcentration operators, as well as external magnetic field controlled directional carrier. However, most of the reported gold shells coated on the magnetic cores had smooth surfaces rather than branched nanostructures with enhanced SERS activity. Here, a novel type of Fe3O4-Au core-shell nanoparticles with branched gold shell was prepared by a seed-mediated method together with the shape induction agent AgNO3, and their growth process and mechanism, properties, as well as morphologically controlled synthesis were also investigated. The branched gold coated magnetic nanoparticles (B-GMNPs) with improved SERS performance were further utilized to build superparamagnetic and SERS-active capturers by assembling tetrahedral DNA onto their surfaces for sandwich-structured detection of cancer-related biomarker miRNA-21. The experimental results indicate that highly sensitive and specific detections can be obtained by the proposed SERS sensing system including B-GMNPs and tetrahedral DNA, and the limit of detection (LOD) of miRNA-21 in serum is 623 amol L−1. These B-GMNPs can be used as good SERS substrates with the functions of external magnetic field controlled sample separation and directional enrichment for effective SERS-based biochemical sensing and detections.

Keywords

magnetic nanoparticles branched Au shell DNA tetrahedron SERS miRNA 

磁核枝杈状金壳纳米颗粒的制备及其在肿瘤核酸标志物SERS检测中的应用研究

摘要

复合SERS活性和超顺磁性的磁核金壳纳米颗粒在SERS传感、样品分离与富集、定向输运等方面已有广泛应用. 已报道的基于磁 核制备的金纳米壳大多为平滑的金壳, 基于磁核生长枝杈状金壳可以提升颗粒的SERS增强性能, 但相关报道甚少. 本文基于种子生长法 并引入定向生长控制剂AgNO3, 合成了一种新型的磁核枝杈状金壳纳米颗粒, 系统地研究了金壳的生长过程与机制、表面形貌的控制生 长以及对SERS性能的影响. 此外, 设计并构建了具有良好的结构刚性、稳定性和空间定位能力的四面体DNA探针, 并将其修饰到磁核枝 杈状金壳纳米颗粒表面构建得到检测基底. 通过在金纳米颗粒表面修饰单链DNA构建SERS探针, 利用碱基互补配对形成“检测基底-目标 核酸-SERS探针”三明治结构复合物, 借助外加磁场分离检测液中的复合物并富集后进行SERS检测, 实现了对于血清中肿瘤核酸标志物 miRNA-21的高灵敏、特异性检测, 检测限达到623 amol L−1.

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (21475064), Sci-tech Support Plan of Jiangsu Province (BE2014719), Program for Changjiang Scholars and Innovative Research Team in University (IRT_15R37), the Research Innovation ProgramforCollegeGraduates of Jiangsu Province (SJZZ15_0107), the Scientific Research Foundation of Nanjing University of Posts and Telecommunications (NY215075), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (YX03001).

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Yanjun Yang (杨琰君)
    • 1
  • Xinyu Jiang (蒋新宇)
    • 1
  • Jie Chao (晁洁)
    • 1
  • Chunyuan Song (宋春元)
    • 1
  • Bing Liu (刘冰)
    • 1
  • Dan Zhu (朱丹)
    • 1
  • Youzhi Sun (孙友志)
    • 1
  • Boyue Yang (杨博玥)
    • 1
  • Qiaowei Zhang (张乔伟)
    • 1
  • Ye Chen (陈叶)
    • 1
  • Lianhui Wang (汪联辉)
    • 1
  1. 1.Key Lab for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)Nanjing University of Posts & TelecommunicationsNanjingChina

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