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Polyamines and amino acids in triticale plants grown on humic acids enriched nutrient solution and treated with UV-B irradiation

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Abstract

The effects of UV-B irradiation and Biomin (a natural substance extracted from coal with active ingredients of humic acids) on the content of endogenous polyamines spermine, spermidine and putrescine, and free amino acids in shoots and roots of young triticale seedlings were investigated. Biomin was added to the nutrient medium 3 days prior to UV-B irradiation. The seedlings were treated with 7.7 kJ m−2 day−1 UV-B light for 4 days. The exposure to UV-B increased total free amino acids, while Biomin application alone did not affect considerably their content. The treatment with UV-B or Biomin alone provoked augmentation of conjugated and bound polyamine (PA) fractions. Data suggest that Biomin alleviates the negative consequences of UV-B stress, manifested by the normalized amino acid and polyamine amounts in the UV-B + Biomin-treated plants. This study demonstrates the protective effect of Biomin on triticale plants against UV-B irradiation, which could be related to alterations in PAs and amino acids pools.

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Acknowledgements

This study was partially funded by the Bulgarian Science Fund, project DMU 03/60. The authors gratefully acknowledge Asya Nikolova and Nina Georgieva from the IPPG-BAS for their excellent technical assistance.

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Authors and Affiliations

  1. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg. 21, 1113, Sofia, Bulgaria

    Iskren Sergiev, Dessislava Todorova & Zornitsa Katerova

  2. National Research Council, Institute of Agricultural Biology and Biotechnology, via Bassini 15, 20133, Milan, Italy

    Ida Brambilla & Sergio Mapelli

  3. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg. 9, 1113, Sofia, Bulgaria

    Svetlana Simova

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  1. Iskren Sergiev
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  2. Dessislava Todorova
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  3. Zornitsa Katerova
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  4. Ida Brambilla
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  5. Sergio Mapelli
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  6. Svetlana Simova
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Correspondence to Dessislava Todorova.

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Sergiev, I., Todorova, D., Katerova, Z. et al. Polyamines and amino acids in triticale plants grown on humic acids enriched nutrient solution and treated with UV-B irradiation. Theor. Exp. Plant Physiol. 30, 153–163 (2018). https://doi.org/10.1007/s40626-018-0110-9

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