The effect of vitamin D supplementation in combination with low-calorie diet on anthropometric indices and androgen hormones in women with polycystic ovary syndrome: a double-blind, randomized, placebo-controlled trial
Polycystic ovary syndrome (PCOS) is known as the most common endocrine disorder in reproductive age women. The aim of this studywas to evaluate the effects of vitamin D supplementation in combination with low-calorie diet on anthropometric indices, reproductive hormones and menstrual regularity in overweight and obese PCOS women.
In this randomized controlled clinical trial, 60 PCOS women with vitamin D insufficiency were randomly assigned to 12 weeks of either (1) weight-loss intervention + 50,000 IU/week oral vitamin D3 or (2) weight-loss intervention + placebo. At the beginning and end of the study, the anthropometric indices, body composition, 25-hydroxyvitamin D, total testosterone, dehydroepiandrosterone sulfate (DHEAS), sex hormone-binding globulin (SHBG) and free androgen index (FAI) were measured and regularity of menses was compared among the two groups.
After 12-week intervention, median of serum 25-hydroxyvitamin D3 significantly increased from 18.5 (10.75–20) ng/ml to 42.69 (34–53.25) ng/ml in vitamin D group compared to placebo group (p < 001). Moreover, there was a significant improvement in frequency regular menstrual cycle (p = 0.01). Mean of weight, body mass index, fat mass, waist and hip circumference and waist-to-hip ratio significantly decreased in both groups, but was not different between two groups. Mean of total testosterone insignificantly decreased from 0.7 to 0.5 ng/ml in vitamin D group (p = 0.18). In addition, we did not observe significant differences regarding DHEAS, FAI and SHBG between two groups.
In women with PCOS, androgen profile did not change with vitamin D supplementation when combined with low-calorie diet, but menstrual frequency significantly improved.
Clinical Trial Registration Number
KeywordsPolycystic ovary syndrome Vitamin D Androgens Low-calorie diet Menstrual cycle Randomized controlled trial
The authors thank all the women that participated in the present study. This study was done as a MS thesis in Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Compliance with ethical standards
Financial support for this study was provided by Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Conflict of interest
The authors declare that they have no conflict of interests.
This research conducted according to Helsinki statement and the protocol was approved by Ethics Committee in Shahid Sadoughi University of Medical Sciences.
All participants completed written informed consent.
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