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Effect of paclobutrazol and cytokinin on growth and source–sink relationship during grain filling of black rice (Oryza sativa L. “Cempo Ireng”)

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Abstract

The black rice (Oryza sativa L. “Cempo Ireng”) contains high amount of anthocyanin which functions as an antioxidant. Cultivation of black rice in Indonesia is still limited because of its long vegetative phase and low yield. Paclobutrazol is a growth retardant that may affect the photosynthate allocation, whereas cytokinin is a plant hormone that could increase the sink strength. This research was aimed to evaluate the effect of paclobutrazol and cytokinin on growth and source–sink relationship during grain filling of black rice. Black rice seeds were sown in the plastic bucket filled with soil and organic fertilizer (3:1 = v/v). Paclobutrazol of 0 ppm (control), 50 ppm or 100 ppm were applied as a foliar spray at 10 weeks after sowing. Cytokinin of 0 M (control) or 10−5 M in 0.8% agar gel was applied twice at 2 weeks after anthesis and the day after by injection in the flag leaf internode lacuna. For each treatment combination 8 replicates were made. Cytokinin enhanced the net photosynthesis, grains growth both in vivo and in vitro, dry weight of 100 grains, percentage of filled grain, the number of endosperm cells, starch and amylose contents in the grain. The flag-leaf area was reduced by paclobutrazol, but paclobutrazol of 50 ppm increased sucrose and amylopectin content in the grain. Paclobutrazol reduced plant height and application of cytokinin could improve the assimilate allocation to developing black rice grain.

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References

  • Alizadeh, O., Haghighi, B. A., & Ordookhani, K. (2010). The effect of exogenous cytokinin application on sinks size of bread wheat (Triticum aestivum). African Journal of Agricultural Research, 5(21), 2893–2898.

    Google Scholar 

  • Ashikari, M., & Sakamoto, T. (2008). Rice yielding and plant hormones. Biotechnology in Agricultural and Forestry, 72, 3009–3320.

    Google Scholar 

  • Black, L. T., & Bagley, E. B. (1978). Determination of oligosaccharides in soybeans by high pressure liquid chromatography using an internal standard. Journal of the American Oil Chemists’ Society, 55(2), 228–232.

    Article  CAS  Google Scholar 

  • Bora, K. K., Munot, J., & Marthur, S. (1991). Metabolic changes associated with paclobutrazol induced rootings in hypocotyl of mung bean. Botany Bulletin Academia Sinica, 32, 9–14.

    CAS  Google Scholar 

  • Bridgemohan, P., & Bridgemohan, R. S. T. (2014). Evaluation of anti-lodging plant growth regulators on the growth and development of rice (Oryza sativa L.). Journal of Cereals and Oilseeds, 5(3), 12–16.

    CAS  Google Scholar 

  • Chen, M. H., & Bergman, C. J. (2007). Method for determining the amylosa content, molecular weights and molar based distribution of degree of plymerization of amylose and fine-structure of amylopectin. Carbohydrate Polymers, 69, 562–578.

    Article  CAS  Google Scholar 

  • Chrastil, J. (1987). Improve colometric determination of amylosa in starches or flours. Carbohydrate Research, 159, 154–158.

    Article  CAS  Google Scholar 

  • Chrastil, J. (1990). Protein-starch interactions in rice grain. Influence of storage on oryzein and starch. Journal of Agriculture and Food Chemistry, 38, 1804–1809.

    Article  CAS  Google Scholar 

  • Chrastil, J. (1993). Changes of oryzenin and starch during preharvest and maturation of rice-grain. Journal of Agriculture and Food Chemistry, 41, 2242–2744.

    Article  CAS  Google Scholar 

  • Daie, J. (1985). Carbohydratae partitioning and metabolism in crops. Horticultural Review, 7, 69–108.

    Google Scholar 

  • Darussalam, Cole, M. A., & Patrick, J. W. (1998). Auxin control of photoassimlate transport to and within developing grain of wheat. Australian Journal of Plant Physiology, 25, 69–77.

    Article  CAS  Google Scholar 

  • Davies, D. J. (2004). Plant hormones. Biosynthesis, signal transduction, action (p. 750). The Netherlands: Kluwer Academic Publishers.

    Google Scholar 

  • Davindra, S., & Longvah, T. (2011). Analysis of digestive carbohybdrate in different varieties of Basmati rice and other popular cereal samples by using HPLC-R1. Word Journal of Dairy & Food Science, 6, 146–151.

    Google Scholar 

  • de Moura, F. B., Marcos R. da S. Vieira, Adriano do N. Simões Sergio L. F. da Silva Damiana C. de Medeiros R.einaldo de A. PaesArthur A. S. de Oliveira Antônio H. C. do Nascimento Walter S. E. Júnior. (2017). Participation of cytokinin on gas exchange and antioxidant enzymes activities. Indian Journal of Plant Physiology, 22(1): 16–29

  • Del Rosario, A. R., Briones, V. P., Vidal, A. J., & Yuliano, B. O. (1968). Composition and endosperm structure of developing and mature rice kernel. Cereal Chemistry, 45, 225–235.

    Google Scholar 

  • Dewi, K., Rizkika, Z. A., & Farida, N. (2016). Effects of blue light and paclobutrazol on seed germination, vegetative growth and yield of black rice (Oryza sativa L. ‘Cempo Ireng’). Biotropia, 23(2), 84–95. https://doi.org/10.11598/btb.2016.23.2.478.

    Article  Google Scholar 

  • Falqueto, A. R., Cassol, D., Megalhaes, A. M., Jr., De Oliveira, A. C., & Bacarin, M. (2000). Physiological analysis of leaf senescence of two rice cultivars with different yield potential. Pesquisa Agropecuária Brasileira, 44(7), 695–700.

    Article  Google Scholar 

  • Frymire, R. M., & Henderson-Cole, J. C. (1992). Effect of uniconazol and limited water on growth, water reaction and mineral nutrition of “Lalandu” Pyracanthe. Journal of Plant Growth Regulation, 11, 227–231.

    Article  CAS  Google Scholar 

  • Gomez, K. A., & Gomez, A. A. (1976). Statistical procedure for agriculture research with emphasize on rice (p. 294). Los Banos Philippines: Internationational Rice Research Institute.

    Google Scholar 

  • Hao, W., & Lin, H. X. (2010). Toward understanding genetic mechanism of complex traits in rice. Journal of Genectic and Genomic, 37, 653–666.

    Article  CAS  Google Scholar 

  • Hua, S., Zhang, Y., Yu, H., Lin, B., Ding, H., Zhang, D., et al. (2014). Paclobutrazol application effects on plant height, seed yield and carbohydrate metabolism in canola. International Journal of Agricultural Biology, 16, 471–479.

    CAS  Google Scholar 

  • Jaleel, C. A., Gopi, R., Azooz, M. M., & Panneerselvam, R. (2009). Leaf anatomical modification in Cataranthus roseus as affected by plant growth promotors and retardants. Global Journal of Molecular Sciences, 4, 1–5.

    Google Scholar 

  • Jeng, T. L., Tseng, T. H., Wang, C. S., Chen, C. L., & Sung, J. M. (2006). Yield and grain uniformity in contrasting rice genotype suitable for different growth evironments. Field Crops Research, 99, 59–66.

    Article  Google Scholar 

  • Juan, G., Luis, A., & David, B. (2006). Isolation and molecular characterization of Makal (Xanthosomayucatanensis) starch. Starch, 58, 300–307.

    Article  Google Scholar 

  • Juliano, B. O. (1992). Structure, chemistry and function of the rice grain and its fractions. CVF Review, 37(10), 772–779.

    CAS  Google Scholar 

  • Keshorekumar, A., Jaleel, C. A., Manivannam, P., Sankar, B., Sridan, D., & Panneerselvan, R. (2007). Comparative effect of different triazole compound on growth, photoshyntetic pigment and carbohydrate metabolism of Solanum rotundifolius. Colloid and Surface: Biointerface, 60, 2017–2212.

    Google Scholar 

  • Lee, S.-T., & Hing, W.-L. (2014). Cytokinin, auxin and absicic acid effect sucrose metabolism induce to de novo shoot callus organogenesis in rice (Oryza sativa L.) callus. Botanical Studies, 54, 2–11.

    Google Scholar 

  • Ling, W. H., Cheng, Q. X., Ma, J., & Wang, T. (2001). Red and black rice decreases artheroscherotic plaque formation and increase antioxidant status in rabbits. Journal of Nutrition, 131, 1421–1426.

    Article  CAS  Google Scholar 

  • Mia, M. A. B., & Shamsuddin, Z. H. (2011). Physio-morphological appraisal of aromatic fine rice (Oryza sativa L.) in relation to yield potential. International Journal of Botany, 7(3), 223–229.

    Article  Google Scholar 

  • Mohapatra, P. K., & Kariali, E. (2008). Time of emergence determines the pattern of dominance rice tiller. Australian Journal of Crop Sciences, 1(2), 53–62.

    Google Scholar 

  • Mohapatra, P. K., Sarkar, R. K., & Kuanar, S. R. (2009). Starch synthesizing enzyme and sink strength of grain of contrasting rice cultivars. Plant Science, 176, 256–263.

    Article  CAS  Google Scholar 

  • Nagai, Y. S., Sakulsinggharoj, C., Edward, G. E., Satoh, H., Greene, T. W., Blakeslee, B., et al. (2009). Control of starch synthesis in cereals: Metabolite analysis of transgenic rice expressing up-regulated cytoplasmic ADP-glucose pyrophosphorylase in developing seed. Plant Cell Physiology, 50(3), 635–643.

    Article  CAS  Google Scholar 

  • Nakamura, Y., & Yuki, K. (1992). Changes in enzymes activities associated with carbohydrate metabolism during the development of rice endosperm. Plant Science, 82, 15–20.

    Article  CAS  Google Scholar 

  • Ookawa, T., Naruoka, Y., Sayama, A., & Hirasawa, T. (2004). Cytokinin effects on ribulose-1,5-bisphosphate carboxylase/oxygenase and nitrogen partitioning in rice during ripening. Crop Science, 44, 2107–2115. https://doi.org/10.2135/cropsci2004.2107.

    Article  CAS  Google Scholar 

  • Oparka, K. J., & Gates, P. J. (1981a). Transport of assimilates in the developing caryopsis of rice (Oryza sativa L.). Ultrastructure of the pericarp vascular bundle and its connection with the aleurone layer. Planta, 151, 561–573.

    Article  CAS  Google Scholar 

  • Oparka, K. J., & Gates, P. J. (1981b). Transport of assimilates in the developing caryopsis of rice (Oryza sativa L.). The pathway of water and assimilate carbon. Planta, 152, 388–396.

    Article  CAS  Google Scholar 

  • Pan, S., Rasul, F., Mo, Z., Duon, M., & Tang, X. (2013). Roles of plant growth regulation on yield, grain qualities and antioxidant enzyme activities in super hibrid rice (Oryza sativa L.). Rice, 6, 1–10.

    Article  Google Scholar 

  • Peleg, Z., Reguera, M., Tumimbang, E., Walia, H., & Blumwald, E. (2011). Cytokinin-mediated source/sink modifications improve drought tolerance and increase grain yield in rice under water-stress. Plant Biotechnology Journal, 9, 747–758.

    Article  CAS  Google Scholar 

  • Rahman, M. M., Lee, K. E., Matin, M. N., Lee, D. S., Yun, J. S., Kim, J. B., et al. (2013). The genetic constitutions of complementary genes Pp and Pb determine the purple color variation in black rice. Journal of Plant Biology, 56, 24–31.

    Article  CAS  Google Scholar 

  • Rijven, A. H. G. C., & Wardlaw, I. F. (1966). Experimental Cell Research, 41, 324.

    Article  CAS  Google Scholar 

  • Roitsch, T., & Ehneb, R. (2000). Regulation of source/sink relation by cytokinin. Plant Growth Regulation, 32, 359–367.

    Article  CAS  Google Scholar 

  • Ruan, J. L., Patrick, J. W., & Weber, H. (2010). Asimilat partitioning and plant development. Molecular Plant Shanghai Editorial, 3(6), 941.

    Article  CAS  Google Scholar 

  • Sadeghi-Shoae, M., Habibi, D., Taleghani, D. F., Paknejad, F., & Kashani, A. (2014). Evaluation the effect of paclobutrazol on bolting, qualitative and quantitative performance in autumn sown-sugar beet genotypes in Moghan region. International Journal of Biosciences, 5(1), 345–354.

    Google Scholar 

  • Sasamoto, H. O., & Suzuki, H. (1979). Activities of enzyme relating starch synthesis and endogenous levels of growth regulator in potato stolon tips during tuberization. Physiologia Plantarum, 45, 320–324.

    Article  Google Scholar 

  • Setia, R. C., Bathal, G., & Setia, N. (1995). Influence of paclobutrazol on growth and yield of Brassica caranata A.Br. Plant Growth Regulation, 16, 121–127.

    Article  CAS  Google Scholar 

  • Sinniah, U. A., Wahyuni, S., Syahputra, B. S. A., & Gantait, S. (2012). A potential retardant for lodging resistance in direct seeded rice (Oryza sativa L.). Canadian Journal of Plant Science, 92, 13–18.

    Article  CAS  Google Scholar 

  • Smeekens, S. (1998). Sugar regulation of gene expression in plants. Current Opinion in Plant Biology, 1, 230–234.

    Article  CAS  Google Scholar 

  • Smith, A. M., & Stitt, M. (2007). Coordination of carbon supply and plant growth. Plant, Cell and Environment, 30, 1126–1149.

    Article  CAS  Google Scholar 

  • Soumya, P. R., Pramod, K., & Madan, P. (2017). Paclobutrazol: A novel plant growth regulator and multi-stress ameliorant. Indian Journal of Plant Physiology, 22(3), 267–278.

    Article  CAS  Google Scholar 

  • Tsegaw, T., Hammes, S., & Robbertse, J. (2005). Paclobutrazol-induced leaf, stem, and root anatomical modifications in potato. HortScience, 40(5), 1343–1346.

    CAS  Google Scholar 

  • Turgeon, R. (2010). The role of phloem loading. Plant Physiology, 152, 1817–1823.

    Article  CAS  Google Scholar 

  • Warner, T., Motyka, V., Strnad, M., & Schmulling, T. (2001). Regulation of plant growth by cytokinin. Proceeding National Academy of Science USA, 98(18), 10487–10492.

    Article  Google Scholar 

  • Wieland, W., & Wampler, R. (1985). Effect of paklobutrazol on growth, photosynthesis and carbohydrate content. Science Horticulturae, 26, 139–147.

    Article  CAS  Google Scholar 

  • Wingler, A., von Schaewen, A., Leegood, R. C., Lea, P. J., & Quick, W. P. (1998). Regulation of leaf senescence by cytokinin, sugars, and light effects on NADH dependent hydroxypyruvate reductase. Plant Physiology, 116, 329–335.

    Article  CAS  Google Scholar 

  • Winkar, V.L. 1998. Effet of nitrogen and cytokinins on photosynthesis and growth of Sorghum (Sorghum bicolor (L). Moench) under Striga hermonthica infestati. Thesis, Agricultural Wegeningen University, The Netherland, 35 pp.

  • Xia, X., Yuhan, T., Mengran, W., & Daqiu, Z. (2018). Effect of paclobutrazol application on plant photosynthetic performance and leaf greenness of herbaceous peony. Horticulturae, 4, 5. https://doi.org/10.3390/horticulturae4010005.

    Article  Google Scholar 

  • Xie, Z., Jiang, D., Dai, T., Jing, Q., & Cao, W. (2004). Effect of exogenous ABA and cytokinin on leaf photosynthesis and grain protein accumulation in wheat ears in vitro. Plant Growth Regulation, 44, 25–32.

    Article  CAS  Google Scholar 

  • Yang, J., & Zhang, J. (2010). Crop management techniques to enhance harvest index in rice. Journal of Experimental Botany, 61(12), 3177–3189. https://doi.org/10.1093/jxb/erq112.

    Article  CAS  PubMed  Google Scholar 

  • Yang, J., Zhang, J., Huang, Z., Wang, Z., Zhu, Q., & Liu, L. (2002). Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice. Annals of Botany, 90, 369–377.

    Article  CAS  Google Scholar 

  • Zhang, H., Chen, T., Wang, Z., Yang, J., & Zhang, J. (2010). Involvement of cytokinins in the grain filling of rice under alternate wetting and drying irrigation. Journal of Experimental Botany, 61(13), 3719–3733.

    Article  CAS  Google Scholar 

  • Zhang, M. W., Guo, B. J., Zhang, R. F., Chi, J. W., Wei, Z. C., Xu, Z. H., et al. (2006). Separation, purification and composition on black rice. Agricultural Sciences in China, 5(6), 431–440.

    Article  Google Scholar 

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Acknowledgements

The present research was financially supported by TP3F research Grant from Faculty of Biology, Gadjah Mada University, Financial year 2013. The authors are thankful to Dr. Maryani for critical review and English editing.

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Authors and Affiliations

  1. Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Yogyakarta, 55281, Indonesia

    Kumala Dewi

  2. Agriculture Faculty, Universitas Tanjungpura, Jl. Prof. Hadari Nawawi, Pontianak, 78121, Indonesia

    Darussalam

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  1. Kumala Dewi
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KD contributed to the experiment design, DS contributed to data analysis and drafted the manuscript. Both authors read and approved the final manuscript.

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Correspondence to Kumala Dewi.

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Dewi, K., Darussalam Effect of paclobutrazol and cytokinin on growth and source–sink relationship during grain filling of black rice (Oryza sativa L. “Cempo Ireng”). Ind J Plant Physiol. 23, 507–515 (2018). https://doi.org/10.1007/s40502-018-0397-1

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