Indian Journal of Plant Physiology

, Volume 23, Issue 1, pp 77–90 | Cite as

Screening soybean genotypes for high temperature tolerance by in vitro pollen germination, pollen tube length, reproductive efficiency and seed yield

  • Kanchan Jumrani
  • Virender Singh Bhatia
  • Govind Prakash Pandey
Original Article


High temperature stress is a major environmental stress and there are limited studies elucidating the impact of high day and night time temperature on reproductive processes in soybean. Twelve soybean genotypes were grown at day/night temperatures of 30/22, 34/24, 38/26 and 42/28 °C with an average temperature of 26, 29, 32 and 35 °C, respectively under green-house conditions. High temperature stress significantly increased duration of flowering and decreased number of flowers and pods formed as compared to ambient temperature. When plants were grown at elevated temperature pollen germination, pollen size and pollen tube length were declined leading to reduced reproductive efficiency which ultimately resulted reduction in seed yield. The average seed yield was maximum (13.2 g/plant) in plants grown under ambient temperature condition. Seed yield was declined by 8, 14, 51 and 65% as the plants were grown at 30/22, 34/24, 38/26 and 42/28 °C as compared to plants grown under ambient temperature conditions, respectively. The genotypes such as NRC 7 and EC 538828 showed less reduction in yield and stable reproductive biology as compared to other genotypes. It is concluded that for heat tolerance in soybean, breeding efforts needs to be focused on improving the reproductive efficiency.


Pollen germination Reproductive efficiency Soybean Temperature Yield 



Kanchan Jumrani would like to acknowledge the Council of Scientific and Industrial Research (CSIR)/University Grants commission (UGC), Government of India (20-06/2010 (i) EU-IV) for providing the financial support in the form of Research Fellowship.


  1. Adriana, G. K., Mercau, J. L., Slafer, G. A., & Sadras, V. O. (2007). Simulated yield advantages of extending post flowering development at the expense of a shorter pre flowering development in soybean. Field Crops Research, 101, 321–330.CrossRefGoogle Scholar
  2. Ali, M., Gupta, S., & Basu, P. S. (2009). Higher levels of warming in North India will affect crop productivity. Hindu Survey of Indian Agriculture, pp. 44–48.Google Scholar
  3. Bhatia, V. S., & Jumrani, K. (2016). A maximin-minimax approach for classifying soybean genotypes for drought tolerance based on yield potential and loss. Plant Breeding, 136, 691–700.CrossRefGoogle Scholar
  4. Bhatia, V. S., Jumrani, K., & Pandey, G. P. (2014a). Developing drought tolerance in soybean using physiological approaches. Soybean Research, 12, 1–19.Google Scholar
  5. Bhatia, V. S., Jumrani, K., & Pandey, G. P. (2014b). Evaluation of the usefulness of senescing agent potassium iodide as a screening tool for tolerance to terminal drought in soybean. Plant Knowledge Journal, 3, 23–30.Google Scholar
  6. Bhatia, V. S., Singh, P., Wani, S. P., Chauhan, G. S., Kesava Rao, A. V. R., Mishra, A. K., et al. (2008). Analysis of potential yields and yield gaps of rain fed soybean in India using CROPGRO-Soybean model. Agriculture and Forest Meteorology, 148, 1252–1265.CrossRefGoogle Scholar
  7. Boyer, J. S., & Westgate, M. E. (2004). Grain yield with limited water. Journal of Experimental Botany, 55, 2385–2394.CrossRefPubMedGoogle Scholar
  8. Cross, R. H., McKay, S. A. B., Mc Hughen, A. G., & Bonham-Smith, P. C. (2003). Heat stress effects on reproduction and seed set in Linum usitatissimum L. (flax). Plant, Cell and Environment, 26, 1013–1020.CrossRefGoogle Scholar
  9. Djanaguiraman, M., Prasad, P. V. V., Boyle, D. L., & Schapaugh, W. T. (2013a). Soybean pollen anatomy, viability and pod set under high temperature stress. Journal of Agronomy and Crop Science, 199, 171–177.CrossRefGoogle Scholar
  10. Djanaguiraman, M., Prasad, P. V. V., & Schapaugh, W. T. (2013b). High day or nighttime temperature alters leaf assimilation, reproductive success, and phosphatidic acid of pollen grain in soybean (Glycine max (L.) Merr.). Crop Science, 53, 1594–1604.CrossRefGoogle Scholar
  11. Gross, Y., & Kigel, J. (1994). Differential sensitivity to high temperature of stages in the reproductive development of common bean (Phaseolus vulgaris L.). Field Crops Research, 36, 201–212.CrossRefGoogle Scholar
  12. Hedhly, A., Hormaza, J. I., & Herrero, M. (2009). Global warming and sexual plant reproduction. Trends in Plant Science, 14, 30–36.CrossRefPubMedGoogle Scholar
  13. Huan, F., Lizhe, A., Ling Ling, T., Zong Dong, H., & Xunling, W. (2000). Effect of enhanced ultraviolet-B radiation on pollen germination and tube growth of 19 Taxa in vitro. Environmental and Experimental Botany, 43, 45–53.CrossRefGoogle Scholar
  14. IPCC. (2013). Summary for policymakers. In: T. F. Stocker, D. Qin, G. K. Plattner, M. Tignor, S. K. Allen, J. Boschung, A. Nauels, Y. Xia, V. Bex, & P. M. Midgley (Eds.), Climate change: The physical science basis. Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge, United Kingdom and New York, USA.Google Scholar
  15. Jagadish, S. V. K., Craufurd, P. Q., & Wheeler, T. R. (2007). High temperature stress and spikelet fertility in rice (Oryza sativa L.). Journal of Experimental Botany, 58, 1627–1635.CrossRefPubMedGoogle Scholar
  16. Jumrani, K., & Bhatia, V. S. (2014). Impact of elevated temperatures on growth and yield of chickpea (Cicer arietinum L.). Field Crops Research, 164, 90–97.CrossRefGoogle Scholar
  17. Jumrani, K., Bhatia, V. S., & Pandey, G. P. (2017). Impact of elevated temperatures on specific leaf weight, stomatal density, photosynthesis and chlorophyll fluorescence in soybean. Photosynthesis Research, 131, 333–350.CrossRefPubMedGoogle Scholar
  18. Kakani, V. G., Prasad, P. V. V., Craufurd, P. Q., & Wheeler, T. R. (2002). Response of in vitro pollen germination and pollen tube growth of groundnut (Arachis hypogaea L.) genotype to temperature. Plant, Cell and Environment, 25, 1651–1661.CrossRefGoogle Scholar
  19. Kakani, V. G., Reddy, K. R., Koti, S., Wallace, T. P., Prasad, P. V. V., Reddy, V. R., et al. (2005). Differences in in vitro pollen germination and pollen tube growth of cotton cultivars in response to high temperature. Annals of Botany, 96, 59–67.CrossRefPubMedPubMedCentralGoogle Scholar
  20. Koti, S., Reddy, K. R., Kakani, V. G., Zhao, D., & Reddy, V. R. (2004). Soybean (Glycine max) pollen germination characteristics, flower and pollen morphology in response to enhanced ultraviolet-B radiation. Annals of Botany, 94, 855–864.CrossRefPubMedPubMedCentralGoogle Scholar
  21. Mall, R. K., Singh, R., Gupta, A., Srinivasan, G., & Rathore, L. S. (2006). Impact of climate change on Indian agriculture: A review. Climate Change, 78, 445–478.CrossRefGoogle Scholar
  22. Peet, M. M., & Willits, D. H. (1998). The effect of night temperature on greenhouse grown tomato yields in warm climate. Agriculture and Forest Meteorology, 92, 191–202.CrossRefGoogle Scholar
  23. Porch, T. G., & Jahn, M. (2001). Effects of high temperature stress on microsporogenesis in heat sensitive and heat tolerant genotypes of Phaseolus vulgaris. Plant, Cell and Environment, 24, 723–731.CrossRefGoogle Scholar
  24. Prasad, P. V. V., Boote, K. J., & Allen, L. H. (2006a). Adverse high tempearture effects on pollen viability, seed-set, seed yield and harvest index of grain-sorghum (Sorghum bicolor (L.) Moench) are more severe at elevated carbon dioxide due to higher tissue temperatures. Agriculture and Forest Meteorology, 139, 237–251.CrossRefGoogle Scholar
  25. Prasad, P. V. V., Boote, K. J., Allen, L. H., Sheehy, J. E., & Thomas, J. M. G. (2006b). Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress. Field Crops Research, 95, 398–411.CrossRefGoogle Scholar
  26. Prasad, P. V. V., Boote, K. J., Allen, H., & Thomas, J. M. G. (2002). Effects of elevated temperature and carbon dioxide on seed-set and yield of kidney bean (Phaseolus vulgaris L.). Global Change Biology, 8, 710–721.CrossRefGoogle Scholar
  27. Prasad, P. V. V., Boote, K. J., Allen, L. H., Jr., & Thomas, J. M. G. (2003). Supra-optimal temperatures are detrimental to peanut (Arachis hypogaea L) reproductive processes and yield at ambient and elevated carbon dioxide. Global Change Biology, 9, 1775–1787.CrossRefGoogle Scholar
  28. Prasad, P. V. V., Craufurd, P. Q., & Summerfield, R. J. (1999). Fruit number in relation to pollen production and viability in groundnut exposed to short episodes of heat stress. Annals of Botany, 84, 381–386.CrossRefGoogle Scholar
  29. Prasad, P. V. V., Craufurd, P. Q., Summerfield, R. J., & Wheeler, T. R. (2000). Effects of short episodes of heat stress on flower production and fruit-set of groundnut (Arachis hypogaea L.). Journal of Experimental Botany, 51, 777–784.PubMedGoogle Scholar
  30. Prasad, P. V. V., Craufurd, P. Q., Summerfield, R. J., Wheeler, T. R., & Boote, K. J. (2001). Influence of high temperature during pre- and post-anthesis stages of floral development on fruit-set and pollen germination in peanut. Australian Journal of Plant Physiology, 28, 233–240.Google Scholar
  31. Prasad, P. V. V., Pisipati, S. R., Momcilovic, I., & Ristic, Z. (2011). Independent and combined effects of high temperature and drought stress during grain filling on plant yield and chloroplast EF-Tu expression in spring wheat. Journal of Agronomy and Crop Science, 197, 430–441.CrossRefGoogle Scholar
  32. Prasad, P. V. V., Pisipati, S. R., Mutava, R. N., & Tuinstra, M. R. (2008a). Sensitivity of grain sorghum to high temperature stress during reproductive development. Crop Science, 48, 1911–1917.CrossRefGoogle Scholar
  33. Prasad, P. V. V., Staggenborg, S. A., & Ristic, Z. (2008b). Impacts of drought and/or heat stress on physiological, developmental, growth and yield processes of crop plants. In: L. H. Ahuja, & S. A. Saseendran (Eds.), Response of crops to limited water: Understanding and modeling water stress effects on plant growth processes, advances in agricultural systems modeling series 1. ASA-CSSA: Madison, WI, USA, 2008, pp. 301–355.Google Scholar
  34. Reddy, K. R., & Kakani, V. G. (2007). Screening capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length. Scientia Horticulturae, 112, 130–135.CrossRefGoogle Scholar
  35. Salem, M. A., Kakani, V. G., Koti, S., & Reddy, K. R. (2007). Pollen based screening of soybean genotypes for high temperatures. Crop Science, 47, 219–231.CrossRefGoogle Scholar
  36. Sato, S., Peet, M. M., & Thomas, J. F. (2000). Physiological factors limit fruit set of tomato (Lycopersicon esculentum Mill.) under chronic high temperature stress. Plant, Cell and Environment, 23, 719–726.CrossRefGoogle Scholar
  37. Young, L. W., Wilen, R. W., & Bonham Smith, P. C. (2004). High temperature stress of Brassica napus during flowering reduces micro and megagametophyte fertility, induces fruit abortion and disrupts seed production. Journal of Experimental Botany, 55, 485–495.CrossRefPubMedGoogle Scholar

Copyright information

© Indian Society for Plant Physiology 2018

Authors and Affiliations

  • Kanchan Jumrani
    • 1
  • Virender Singh Bhatia
    • 1
  • Govind Prakash Pandey
    • 2
  1. 1.Indian Institute of Soybean ResearchIndoreIndia
  2. 2.School of Life SciencesDAVVIndoreIndia

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