In vitro propagation of Plum (Prunus salicina) cv. ‘Santa Rosa’ and assessment of genetic stability using RAPD markers
- 26 Downloads
Prunus necrotic ring spot virus (PNRSV), Cherry leaf roll virus (CLRV) and Apple chlorotic leaf spot virus (ACLSV) indexed tree of Japanese plum Santa Rosa was used as the source of explants in our studies. Shoot buds were collected and cultured on Murashige and Skoog (MS) medium with different concentrations of benzyl adenine (BA) and gibberellic acid (GA3) for in vitro culture establishment. Maximum per cent establishment (75.92%) was achieved on MS medium supplemented with 2.0 mg L−1 BA and 0.5 mg L−1 GA3. Maximum shoot multiplication was achieved on MS medium fortified with 0.5 mg L−1 BA, 0.1 mg L−1 GA3, 0.05 mg L−1 IBA and 10 mg L−1 casein hydrolysate. For in vitro rooting shoots were pretreated on half strength liquid MS medium containing 5.0 mg L−1 IBA for 24 h followed by transfer to MS basal medium resulting in 79.80% rooting. In vitro raised plantlets were hardened with 80–85% survival after 1 month and were successfully transferred to the field after 1 year with 100% survival. On RAPD analysis, all the amplified products were monomorphic in micropropagated plants and were similar to the mother plant. The micropropagation protocol developed is appropriate for mass clonal propagation of Santa Rosa.
KeywordsVirus indexing Stone fruit DAS ELISA In vitro shoot multiplication RAPD markers
The authors are thankful to the Department of Biotechnology, Ministry of Science and Technology, Government of India for the research grant under the project ‘Biotechnological interventions for establishment of own rooted progeny orchard of some stone fruits’.
- Arab, M. M., Yadollahi, A., Shojaeiyan, A., Shokri, S., & Ghojah, S. M. (2014). Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G × N15 (hybrid of almond × peach) vegetative rootstock. Journal of Genetic Engineering and Biotechnology, 12, 81–87.CrossRefGoogle Scholar
- Calayan, K., Serçe, Ç. U., Gazel, M., & Jelkmann, W. (2006). Detection of four apple viruses by ELISA and RT-PCR assays in Turkey. Turkish Journal of Agriculture and Forestry, 30, 241–246.Google Scholar
- Mandic, B., Matic, S., Rwahnih, M. A. L., Jelkmann, W., & Myrta, A. (2007). Viruses of sweet and sour cherry in Serbia. Journal of Plant Pathology, 89, 103–108.Google Scholar
- Minaev, V. A., Verzilin, A. V., & Vysotskii, V. A. (2003). Efficiency of clonal micropropagation and quality of improved rootstock material of apple. Sadovodstva-i-Vinogradarstvo, 4, 12–13.Google Scholar
- Rana, T., Chandel, V., Hallan, V., & Zaidi, A. A. (2007). Molecular evidence of Applechlorotic leaf spot virus in wild and cultivated apricot in Himachal Pradesh, India. Journal of Plant Pathology, 89, 72–76.Google Scholar
- Soliman, H. I. A. (2012). In vitro propagation of apricot (Prunus armeniaca L.) and assessment of genetic stability of micropropagated plants using RAPD analysis. World Applied Sciences Journal, 19, 674–687.Google Scholar
- Soni, M., Thakur, M., & Modgil, M. (2011). In vitro multiplication of Merton I. 793—an apple rootstock suitable for replantation. Indian Journal of Experimental Biology, 10, 362–368.Google Scholar
- Te-Chato, S., Hilae, A., & In-Peuy, K. (2008). Effects of cytokinin types and concentrations on growth and development of cell suspension culture of oil palm. Journal of Agricultural Technology, 4, 157–161.Google Scholar
- Vinthehalter, B., & Neskovic, M. (1992). Factors affecting in vitro propagation of quince (Cydonia oblonga Mall.). Journal of Horticultural Sciences, 67, 35–43.Google Scholar
- Yildirim, H., Onay, A., Tilkat, E., & Aktürk, Z. (2011). Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture. Turkish Journal of Agriculture and Forestry, 35, 55–59.Google Scholar
- Zou, Z. N. (2010). Micropropagation of Chinese Plum (Prunus salicina Lindl.) using mature stem segments. Notulae Botanicae Horti Agrobotanici Cluj, 38, 214–218.Google Scholar