Advertisement

Indian Journal of Plant Physiology

, Volume 23, Issue 1, pp 40–47 | Cite as

Physiological responses of commercial sugarcane (Saccharum spp. hybrids) varieties to moisture deficit stress tolerance

  • Manel Dapanage
  • Sumangala Bhat
Original Article
  • 105 Downloads

Abstract

Physiological responses of 52 commercial sugarcane varieties (Saccharum sp. hybrids) were studied by withholding irrigation after 2 months of growth under green house conditions. Based on the time taken for appearance of wilting symptoms after exposure to moisture deficit stress, varieties were grouped into different groups and susceptible (Co 775 and Co 99010) and highly tolerant (Co 94008, CoT 8201 and ISH 100) varieties were identified. Physiological parameters such as proline, RWC and chlorophyll content were measured 8 days after stress induction and the changes were recorded under unstressed and stressed conditions. RWC and chlorophyll content under stress condition were positively correlated with the time taken for appearance of wilting symptoms. Reduction in RWC and chlorophyll content under stress condition was negatively correlated with the time taken for appearance of wilting symptoms. Hence, RWC and chlorophyll content can be suggested as a good indicator of stress tolerance.

Keywords

Correlation Moisture deficit stress Physiological responses Sugarcane 

Notes

Acknowledgements

We acknowledge the Indian Council for Cultural Relations, New Delhi for the Financial Assistance in the form of Fellowship to the first author. We also thank Dr. S. B. Patil, Head, ARS, Sankeshwar for a sharing sugarcane varieties and Dr. R.V. Koti for his valuable suggestions while planning the experiment.

References

  1. Altinkut, A., Kazan, K., Ipekci, Z., & Gozukirmizi, N. (2001). Tolerance to paraquat is correlated with the traits associated with water stress tolerance in segregating F2 populations of barley and wheat. Euphytica, 121, 81–86.CrossRefGoogle Scholar
  2. Bates, L. S., Waldren, R. P., & Teare, J. D. (1973). Rapid determination of free proline for water use studies. Plant and Soil, 39, 205–208.CrossRefGoogle Scholar
  3. Bayoumi, T. Y., Eid, M. H., & Metwali, E. M. (2008). Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology, 7, 2341–2352.Google Scholar
  4. Black, C. A. (1965). Methods of soil analysis: Part I physical and mineralogical properties. Madison, WI: American Society of Agricultural and Biological Engineers.Google Scholar
  5. Brestic, M., Cornic, G., Fryer, M. J., & Baker, N. R. (1995). Does photorespiration protect the photosynthetic apparatus in French bean leaves from photoinhibition during drought stress. Planta, 196, 450–457.CrossRefGoogle Scholar
  6. Ceh, B., Cerenak, A., & Majer, D. (2009). Potassium and proline content in hop leaves as biochemical marker for drought stress tolerance. Hmeljarski Bilten, 16, 43–52.Google Scholar
  7. Centritto, M., Lauteri, M., Monteverdi, M. C., & Serraj, R. (2009). Leaf gas exchange, carbon isotope discrimination, and grain yield in contrasting rice genotypes subjected to water deficits during the reproductive stage. Journal of Experimental Botany, 60, 2325–2339.CrossRefPubMedGoogle Scholar
  8. Chaves, M. M., Pereira, J. S., Maroco, J., Rodriguez, M. L., & Ricardo, C. P. (2002). How plants cope with water stress in the field. Photosynthesis and growth. Annals of Botany, 89, 907–916.CrossRefPubMedPubMedCentralGoogle Scholar
  9. Colom, M. R., & Vazzana, C. (2003). Photosynthesis and PSII functionality of drought-resistant and droughtsensitive weeping lovegrass plants. Environmental and Experimental Botany, 49, 135–144.CrossRefGoogle Scholar
  10. Davies, W. J., Wilkinson, S., & Loveys, B. (2002). Stomatal control by chemical signaling and the exploitation of this mechanism to increase water use efficiency in agriculture. New Phytologist, 153, 449–460.CrossRefGoogle Scholar
  11. de Silva, M. A., John, L. J., Sharma, V., da Jorge, A. G. S., Caputo, D. M. M., Guimaraes, E. R., & Ferro, M.I.T. (2011). Use of physiological parameters in screening drought tolerance in sugarcane genotypes. Sugar Tech, 13(3), 191–197.CrossRefGoogle Scholar
  12. Gomathi, R., & Vasantha, S. (2010). Screening for drought tolerance in sugarcane. Extension publication no 180, Sugarcane Breeding Institute, Coimbatore, India.Google Scholar
  13. Gomathi, R., Vasantha, S., Hemaprabha, G., Alarmelu, S., & Shanthi, R. M. (2011). Evaluation of elite sugarcane clones for drought tolerance. Journal of Sugarcane Research, 1(1), 55–62.Google Scholar
  14. Graça, J.P., Rodrigues, F.A., Farias, J.R.B., Oliveira, M.C.N., Hoffmann-Campo, C.B., & Zingaretti, S.M. (2010). Physiological parameters in sugarcane cultivars submitted to water deficit. Brazilian Journal of Plant Physiology, 22(3), 189–197.CrossRefGoogle Scholar
  15. Hanson, A. D., Nelsen, C. E., Pedersen, A. R., & Everson, E. H. (1979). Capacity for proline accumulation during water stress in barley and its implications for breeding for drought resistance. Crop Science, 19, 489–493.CrossRefGoogle Scholar
  16. Hemaprabha, G., Natarajan, U. S., Balasundaram, N., & Singh, N. K. (2006). STMS based genetic divergence among common parents and its use in identifying productive cross combinations for varietal evolution in sugarcane (Saccharum sp.). Sugarcane International, 24(6), 22–27.Google Scholar
  17. Hemaprabha, G., & Swapna, S. (2012). Genetic diversity and selection among drought tolerant genotypes of sugarcane using microsatellite markers. Sugar Tech, 14(4), 327–333.CrossRefGoogle Scholar
  18. Hemaprabha, G., Swapna, S., Leena, D. L., Sajitha, B., & Venkataramana, S. (2013). Evaluation of drought tolerance potential of elite genotypes and progenies of sugarcane (Saccharum sp. hybrids). Sugar Tech, 15(1), 9–16.CrossRefGoogle Scholar
  19. Hien, D. T., Jacobs, M., Angenon, G., Hermans, C., & Thu, T. T. (2003). Proline accumulation and 1- pyrroline-5-carboxylate synthetase gene properties in three rice cultivars differing in salinity and drought tolerance. Plant Science, 165, 1059–1068.CrossRefGoogle Scholar
  20. Ilahi, I., & Dorffling, K. (1982). Changes in abscisic acid and proline levels in maize varieties of different drought resistance. Physiologia Plantarum, 55, 129–135.CrossRefGoogle Scholar
  21. Jamaux, I., Steinmertz, A., & Belhassen, E. (1997). Looking for molecular and physiological markers of osmotic adjustment in sunflower. New Phytologist, 137, 117–127.CrossRefGoogle Scholar
  22. Jangpromma, N., Songsri, P., Thammasirirak, S., & Jaisil, P. (2010). Rapid assessment of chlorophyll content in sugarcane using a SPAD chlorophyll meter across different water stress conditions. Asian Journal of Plant Sciences, 9, 368–374.CrossRefGoogle Scholar
  23. Korir, P., Nyabundi, J., & Kimurto, P. (2006). Genotypic response of common bean (Phaseolus vulgaris. L). to moisture stress condition in Kenya. Asian Journal of Plant Sciences, 5, 24–32.CrossRefGoogle Scholar
  24. Ma, Q., Turner, D. W., Levy, D., & Cowling, W. A. (2004). Solute accumulation and osmotic adjustment in leaves of Brassica oilseeds in response to soil water deficit. Australian Journal of Agricultural Research, 55, 939–945.CrossRefGoogle Scholar
  25. Manivannan, P., Jaleel, C. A., Chang-Xing, Z., Somasundaram, R., & Azooz, M. M. (2008). Variations in growth and pigment composition of sunflower varieties under early season drought stress. Global Journal of Molecular Sciences, 3, 50–56.Google Scholar
  26. Matin, M.A., Jarvis, H., Brown, A., & Ferguson, H. (1989). Leaf water potential, relative water content, and diffusive resistance as screening techniques for drought resistance in Barley. Agronomy Journal, 81(1), 100–105.CrossRefGoogle Scholar
  27. Moore, P. H. (1987). Breeding for stress resistance. In D. J. Heinz (Ed.), Sugarcane improvement through breeding (pp. 503–542). Amsterdam: Elsevier.CrossRefGoogle Scholar
  28. Munawarti, A., Endang, S. T., Paul, H., & Sismindari, K. (2013). Tolerance of accessions of Glagah (Saccharum spontaneum) to drought stress and their accumulation of proline. American Journal of Agricultural and Biological Sciences, 8(1), 1–11.CrossRefGoogle Scholar
  29. Naser, L., Kourosh, V., Bahman, K., & Reza, A. (2010). Soluble sugars and proline accumulation play a role as effective indices for drought tolerance screening in Persian walnut (Juglans regia L.) during germination. Fruits, 65, 97–112.CrossRefGoogle Scholar
  30. O’Neill, P. M., Shanahan, J. F., & Schepers, J. S. (2006). Use of chlorophyll fluorescence assessments to differentiate corn hybrid response to variable water conditions. Crop Science, 46, 681–687.CrossRefGoogle Scholar
  31. Panse, V. G., & Sukhatme, P. V. (1967). Statistical methods for agricultural workers (pp. 167–174). New Delhi: ICAR Publication.Google Scholar
  32. Ramesh, P. (2000). Effect of different levels of drought during the formative phase on growth parameters and its relationship with dry matter accumulation in sugarcane. Journal of Agronomy and Crop Science, 185(2), 83–89.CrossRefGoogle Scholar
  33. Rao, K. C., & Asokan, S. (1978). Studies of free proline association to drought resistance in sugar cane. Sugar Journal, 40, 23–24.Google Scholar
  34. Silva, M. A., Jifon, J. L., Da Silva, J. A. G., & Sharma, V. (2007). Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology, 19, 193–201.CrossRefGoogle Scholar
  35. Silva, M. A., Silva, J. A. G., Enciso, J., Sharma, V., & Jifon, J. (2008). Yield components as indicators of drought tolerance of sugarcane. Scientia Agricola, 65, 620–627.CrossRefGoogle Scholar
  36. Swapna, S., & Hemaprabha, G. (2010). Identification of two new drought specific candidate genes in sugarcane (Saccharum spp.). Electronic Journal of Plant Breeding, 1(4), 1164–1170.Google Scholar
  37. Turner, N. C., Wright, G. C., & Siddique, K. H. M. (2001). Adaptation of grain legume (pulses) to water limited environments. Advances in Agronomy, 71, 193–231.CrossRefGoogle Scholar
  38. Vajrabhaya, M., Kumpun, W., & Chadchawan, S. (2001). The solute accumulation: The mechanism for drought tolerance in RD23 rice (Oryza sativa L.) lines. Science Asia, 27, 93–97.CrossRefGoogle Scholar
  39. Venkataramana, S., Gururajarao, P., & Naidu, K.M. (1986). The effects of water stress during the formative phase on stomatal resistance and leaf water potential and its relationship with yield in ten sugarcane varieties. Field Crops Research, 13, 345–353.CrossRefGoogle Scholar
  40. Zhao, D., Glaz, B., & Comstock, J. C. (2010). Sugarcane response to water-deficit stress during early growth on organic and sand soils. American Journal of Agricultural and Biological Sciences, 5, 403–414.CrossRefGoogle Scholar

Copyright information

© Indian Society for Plant Physiology 2017

Authors and Affiliations

  1. 1.Department of Biotechnology, College of AgricultureUniversity of Agricultural Sciences, DharwadDharwadIndia
  2. 2.Division of Crop ImprovementSugarcane Research InstituteUda WalaweSri Lanka
  3. 3.Department of Genetics and Plant BreedingCollege of AgricultureHanumanamatii, HaveriIndia

Personalised recommendations