Comparison of biochemical and molecular responses of two Brassica napus L. cultivars differing in drought tolerance to salt stress
- 81 Downloads
In this study, levels of MDA, total protein, soluble sugars, and enzymes activity including POX, APX, and CAT were measured in 12 dSm−1 NaCl treated seedlings belong to Licord (drought sensitive cultivar) and SLM046 (drought tolerant cultivar) of Brassica napus. The results showed that salinity increased the amount of MDA, soluble sugars and total protein in both cultivars. Two cultivars partially displayed different trend concerning enzymatic activities under salt stress experiment. Moreover, transcript abundance of four genes involved in signal transduction pathway including Auxin responsive protein, Protein kinase, MAPK3 and MAPK4 were explored at 0, 3, 6, 12 and 24 h under 12 dSm−1 NaCl treatment using RT-PCR approach. The molecular analyses of Licord cultivar revealed the lowest accumulation of all genes after 6 h exposure to NaCl except MAPK3 which was detected at the highest level at this time point. Molecular results of SLM046 cultivar showed that maximum expression of all genes occurred after 6 h treatment except MAPK3 which showed the lowest transcript at 6 h. Our studies indicate better response of SLM046 cultivar to salinity condition compared to Licord cultivar.
KeywordsBrassica napus L. Biochemical parameters Gene expression Molecular parameters Salinity
Authors are also thankful to Biotechnology Research Center of Urmia University for technical support of this work.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Aebi, H. (1984). Catalase in vitro. In L. Packer (Ed.), Methods in enzymology (pp. 121–126). San Diego, CA: Academic Press Inc.Google Scholar
- Azevedo Neto, A. D., Prico, J. T., Eneas-Filho, J., Braga de Abreu, C. E., & Gomes-Filho, E. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environmental and Experimental Botany, 56, 87–94.CrossRefGoogle Scholar
- Hernandez, J. A., Jimenez, A., Mullineaux, P., & Sevilla, F. (2000). Tolerance of pea (Pisum sativum) to long term salt stress is associated with induction of antioxidant defences. Plant Cell and Environment, 23, 853–862.Google Scholar
- Kochert, G. (1978). Carbohydrate determination by phenol-sulfuric acid method. In J. A. Hellebust & J. S. Craige (Eds.), Handbook of physiological methods: physiological and biochemical methods (pp. 95–97). London: Cambridge University Press.Google Scholar
- Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22, 867–880.Google Scholar
- Shirani Rad, A. H., Naeemi, M., & Nasr Esfahani, S. H. (2010). Evaluation of terminal drought stress tolerance in spring and winter rapeseed genotypes. Iranian Journal of Crop Sciences, 12, 112–126. (in Persian).Google Scholar
- Smirnoff, N. (1995). Antioxidant system and plant responses to the environment. In N. Smirnoff (Ed.), Environment and plant metabolism: Flexibility and acclimation (pp. 217–243). Oxford: Bios Scientific Publishers.Google Scholar