Abstract
Ten wilt resistant and three wilt susceptible chickpea genotypes were subjected to drought stress at pre and post flowering stages along with four drought tolerant and three drought susceptible checks by withholding irrigation. The three wilt resistant chickpea genotypes viz., GJG 0922, JG 24 and JG 2001-4 recorded significant increase in proline and glycine betaine accumulation and SOD, APX, GPX, CAT activities under water-deficit stress at preflowering stage. The mean leaf proline content of the control genotypes was 23.72 µmol g−1 DW and increased to 56.73 µmol g−1 DW under drought stress at preflowering stage. Based on the level of osmolyte accumulation, induction of antioxidative enzymes, five chickpea genotypes viz., JG 552476, GJG 0922, JG 24, JG 2001-4 and GJG 0919 could be categorized as drought tolerant. Chickpea genotype PBG 5 which recorded 26.8% wilt incidence and exhibited higher induction of antioxidative enzymes was rolerant to drought stress at preflowering stage while the three wilt resistant genotypes viz., BCP 2010-1, GJG 0904 and IPC 1048 appeared to be drought susceptible.
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References
Ahmad, F., Gaur, P., & Croser, J. (2005). Chickpea (Cicer arietinum L.). In R. Singh & P. Jauhar (Eds.), Genetic resources, chromosome engineering and crop improvement-grain legumes (pp. 185–214). Boca Raton: CRC Press.
Ahmed, I. M., Dai, H., Zheng, W., Cao, F., Zhang, G., Sun, D., et al. (2013). Genotypic differences in physiological characteristics in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley. Plant Physiology and Biochemistry, 63, 49–60.
Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205–207.
Cevik, S., Yildizli, A., Yandim, G., Goksu, H., Gultekin, M. S., Degera, A. G., et al. (2014). Some synthetic cyclitol derivatives alleviate the effect of water deficit in cultivated and wild-type chickpea species. Journal Plant Physiology, 171, 807–816.
Costa, H., Gallego, S. M., & Tomaro, M. L. (2002). Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Science, 162, 939–945.
Farjam, S., Siosemardeh, A., Kazemi-Arbat, H., Yarnia, M., & Rokhzadi, A. (2014). Response of chickpea (Cicer arietinum L.) to exogenous salicylic acid and ascorbic acid under vegetative and reproductive drought stress conditions. Applied Botany Food Quality, 87, 80–86.
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185–212.
Ghanbari, A. A., Shakiba, M. R., Toorchi, M., & Choukan, R. (2013). Morpho-physiological responses of common bean leaf to water deficit stress. European Journal of Experimental Biology, 3, 487–492.
Hasegawa, P. M., Bressan, R. A., Zhu, J. K., & Bohnert, H. J. (2000). Plant cellular and molecular responses to high salinity. Plant Molecular Biology, 51, 463–499.
Henderson, C. R. (1952). Specific and general combining ability. In J. W. Gowen (Ed.), Heterosis. Ames, IA: Iowa State College Press.
Kadkhodaie, A., Zahedi, M., Razmjoo, J., & Pessarakli, M. (2014). Changes in some anti-oxidative enzymes and physiological indices among sesame genotypes (Sesamum indicumL.) in response to soil water deficits under field conditions. Acta Physiologiae Plantarum, 36, 641–650.
Kaur, K., Kaur, N., Gupta, A. K., & Singh, I. (2013). Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions. Plant Growth Regulator, 70, 49–60.
Lugojan, C., & Ciulca, S. (2011). Evaluation of relative water content in winter wheat. Journal of Horticulture Forestry and Biotechnology, 15, 173–177.
Meena, N., Ali, K., Dehmukh, P. S., & Tyagi, A. (2014). Effect of heat stress on physio-biochemical characteristics of chickpea (Cicer arietinum) genotypes. Indian Journal of Agricultural Sciences, 84, 401–406.
Mohammadi, A., Davood, H., Mahyar, R., & Mafakheri, S. (2011). Effect of drought stress on antioxidant enzymes activity of some chickpea cultivars. American-Eurasian Journal of Agriculture and Environmental Science, 11, 782–785.
Munne-Bosch, S., & Penuelas, J. (2003). Photo-and antioxidative protection, and a role for salicylic acid during drought and recovery in field-grown Phillyrea angustifolia plants. Planta, 217, 758–766.
Oberoi, H. K., Gupta, A. K., Kaur, S., & Singh, I. (2014). Stage specific upregulation of antioxidant defence system in leaves for regulating drought tolerance in chickpea. Journal of Applied and Natural Science, 6, 326–337.
Panse, V. G., & Sukhatme, P. V. (1985). Statistical methods for agricultural workers. New Delhi: ICAR publication.
Prochazkova, D., Sairam, R. K., Srivastava, G. C., & Singh, D. V. (2001). Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Science, 161, 765–771.
Rabert, G. A., Manivannan, P., Somasundaram, R., & Panneerselvam, R. (2014). Triazole compounds alter the antioxidant and osmoprotectant status in drought stressed Helianthus annuus L. plants. Emirates Journal of Food and Agriculture, 26, 265–276.
Raheleh, R., Ramazanali, K. N., Ali, G., Abdolreza, B., Farzaneh, N., & Masoud, R. (2012). Use of biochemical indices and antioxidant enzymes as a screening technique for drought tolerance in chickpea genotypes (Cicer arietinum L.). African Journal of Agricultural Research, 7, 5372–5380.
Randhawa, N., & Kaur, J. (2015). Antioxidant responses of Chickpea genotypes exposed to moisture stress. International Journal of Advance Research, 3, 950–955.
Ryan, J. G. (1997). A global perspective on pigeon pea and chickpea sustainable production systems: Present status and future potential. In A. N. Asthana & M. Ali (Eds.), Recent advances in pulses research (pp. 1–31). Kanpur: Indian Society of Pulses Research and Development, IIPR.
Singh, N. P. & Sewak, S. (2013). Global perspective of chickpea research. In AICRP on chickpea (pp. 8–13).
Singh, S., Verma, A., & Dubey, V. K. (2012). Effectivity of anti-oxidative enzymatic system on diminishing the oxidative stress induced by aluminium in chickpea (Cicer arietinum L.) seedlings. Brazilian Journal of Plant Physiology, 24, 47–54.
Stumpf, D. K. (1984). Quantification and purification of quarternary ammonium compounds from halophyte tissue. Plant Physiology, 75, 273–274.
Wu, G., Zhou, Z., Chen, P., Tang, X., Shao, H., & Wang, H. (2014). Comparative ecophysiological study of salt stress for wild and cultivated soybean species from the yellow river delta, China. The Scientific World Journal, 10, 1–13.
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Dalvi, U.S., Naik, R.M. & Lokhande, P.K. Antioxidant defense system in chickpea against drought stress at pre- and post- flowering stages. Ind J Plant Physiol. 23, 16–23 (2018). https://doi.org/10.1007/s40502-017-0322-z
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DOI: https://doi.org/10.1007/s40502-017-0322-z