Abstract
Investigations on mechanical properties of biological cells especially cancer cells can considerably help recognizing various types of cancers. In this paper, we have concentrated on finding mechanical properties of breast cancer cell (MCF-7), elastic and viscoelastic, using atomic force microscopy. Initially, topography and apparent properties of the MCF-7 cell are studied, then the results are analyzed and compared with the literature to ensure the validity. After accurate diagnosis of MCF-7 cells, force–indentation curves for thirty-one cells, each in three different points, are obtained and the elasticity module of each point is calculated using Hertz and Dimitriadis theories. To ensure about the accuracy of experimental data, some statistical analysis is done to extract distribution functions for elasticity module of each theory. Due to the importance of adhesion force in the friction force, the purpose of this section is to determine adhesion changes in different points of the cell. In the next step, spring and viscosity force gradients and consequently stiffness and viscosity in different indentation depths are measured and finally appropriate creep function is extracted for viscoelastic behavior of MCF-7 using the Kelvin–Voigt model.
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References
Rodriguez ML, McGarry PJ, Sniadecki NJ (2013) Review on cell mechanics: experimental and modeling approaches. Appl Mech Rev 65:e060801
Korayem MH, Khaksar H, Taheri M (2013) Modeling of contact theories for the manipulation of biological micro/nanoparticles in the form of circular crowned rollers based on the atomic force microscope. J Appl Phys 114:1–13
Weisenhorn AL, Khorsandi M, Kasas S, Gotzos V, Butt HJ (1993) Deformation and height anomaly of soft surfaces studied with an AFM. Nanotechnology 4:106–113
Vinckier A, Semenza G (1998) Measuring elasticity of biological materials by atomic force microscopy. FEBS Lett 430:12–16
Ikai A, Afrin R, Sekiguchi H, Okajima T, Alam MT (2003) Nano-mechanical methods in biochemistry using atomic force microscopy. Curr Nanosci 4:181–193
Park S, Lee YJ (2013) Nano-mechanical compliance of Müller cells investigated by atomic force microscopy. Int J Biol Sci 9:546–554
Louey MD, Mulvaney P, Tewart PJS (2001) Characterization of adhesional properties of lactose carriers using atomic force microscopy. J Pharm Biomed Anal 25:559–567
Kasas S, Longo G, Dietler G (2013) Mechanical properties of biological specimens explored by atomic force microscopy. J Phys D Appl Phys 46:1–12
Li M, Liu L, Xi N, Wang Y, Dong Z, Xiao X, Zhang W (2012) Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells. Sci China Life Sci 55:968–973
Li QS, Lee GYH, Ong CN, Lim CT (2008) AFM indentation study of breast cancer cells. Biochem Biophys Res Commun 374:609–613
Faria EC, Ma N, Gazi E, Gardner P, Brown M, Clarke NW, Snook RD (2008) Measurement of elastic properties of prostate cancer cells using AFM. Analyst 133:1498–1500
Hui CY, Baney JM (1998) Contact mechanics and adhesion of viscoelastic spheres. Langmuir 14:6570–6578
Cartagena A, Raman A (2014) Local viscoelastic properties of live cells investigated using dynamic and quasi static atomic force microscopy methods. Biophysical 106:1033–1043
Zhai M, McKenna GB (2014) Viscoelastic modeling of nano-indentation experiments: a multi-curve method. J Polym Sci Part 52:633–639
Siamantouras E, Hills E, Squires PE, Liu K (2014) Nano-mechanical investigation of soft biological cell adhesion using atomic force microscopy. Cell Mol Bioeng 8:22–31
Puech H, Taubenberger A, Ulrich F, Krieg M, Muller DJ, Heisenberg CP (2005) Measuring cell adhesion forces of primary gastrulating cells from zebra-fish using atomic force microscopy. J Cell Sci 118:4199–4206
Hertz H (1881) Über die Berührung fester elastischer Körper. Journal für die reine und angewandte Mathematik 92:156–171
Dimitriadis EK, Horkay F, Maresca J, Kachar B, Chadwick RS (2002) Determination of elastic moduli of thin layers of soft material using the atomic force microscope. Biophys J 82:2798–2810
Stegemann B, Backhaus H, Kloss H, Santner E (2007) Spherical AFM probes for adhesion force measurements on metal single crystals. Mod Res Educ Top Microsc 64:820–827
Çolak A (2013) Measuring adhesion forces between hydrophilic surfaces with atomic force microscopy using flat tips. University of Twente, Enschede. https://doi.org/10.3990/1.9789036516129
Geltmeier A, Rinner B, Bade D, Meditz K, Witt R, Bicker U, Bludszuweit Philipp C, Maier P (2015) Characterization of dynamic behavior of MCF7 and MCF10A cells in ultrasonic field using modal and harmonic analyses. PLoS ONE 10:e0134999
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Technical Editor: Estevam Barbosa Las Casas.
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Korayem, M.H., Sooha, Y.H. & Rastegar, Z. MCF-7 cancer cell apparent properties and viscoelastic characteristics measurement using AFM. J Braz. Soc. Mech. Sci. Eng. 40, 297 (2018). https://doi.org/10.1007/s40430-018-1214-5
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DOI: https://doi.org/10.1007/s40430-018-1214-5