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Transferability of nuclear microsatellite markers to the atmospheric bromeliads Tillandsia recurvata and T. aeranthos (Bromeliaceae)

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Abstract

The genus Tillandsia L. is the most diversified genus of Bromeliaceae and represents one of the most specialized cases of vascular epiphytism: the ‘atmospheric bromeliads.’ Such great diversity and ecological specialization make it an interesting model for evolutionary and population genetics studies. Here we report the cross-transferability of SSR markers isolated from other bromeliad species to Tillandsia recurvata (L.) L. and T. aeranthos (Loisel.) Desf., epiphytes with great abundance in both natural and anthropogenic-modified environments, but with contrasting patterns of geographic distribution and mating systems. We tested a total of 27 microsatellite markers and successfully amplified seven polymorphic markers in T. recurvata and T. aeranthos . We then described cross-amplified markers in two populations per species, sampled in both anthropogenic-transformed and natural environments. T. recurvata presented lower allelic richness and heterozygosities, and greater inbreeding coefficient values. Such differences clearly reflect their contrasting mating systems (self-fertilizing in T. recurvata versus self-incompatible in T. aeranthus). The set of cross-amplified microsatellite markers described here will be a helpful tool to address a range of evolutionary and ecological questions.

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Acknowledgements

This study received financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2014/15588-6, 2014/08087-0, 2016/04396-4, 2016/03777-4), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 471756/2013-0).

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Author notes

  1. Cleber Juliano Neves Chaves and Felipe Aoki-Gonçalves have contributed equally to this work.

Authors and Affiliations

  1. Programa de Pós-graduação em Ecologia e Biodiversidade, Instituto de Biociências, Universidade Estadual Paulista (Unesp), Rio Claro, 13506-900, Brazil

    Cleber Juliano Neves Chaves, Felipe Aoki-Gonçalves, Bárbara Simões Santos Leal, Davi Rodrigo Rossatto & Clarisse Palma-Silva

  2. Departamento de Biologia, Universidade Estadual Paulista (Unesp), Jaboticabal, 14884-900, Brazil

    Davi Rodrigo Rossatto

  3. Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083-862, Brazil

    Clarisse Palma-Silva

Authors
  1. Cleber Juliano Neves Chaves
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  2. Felipe Aoki-Gonçalves
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  3. Bárbara Simões Santos Leal
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  4. Davi Rodrigo Rossatto
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  5. Clarisse Palma-Silva
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Contributions

CP-S designed the study. CJNC and FA-G performed the experiments and analyzed the data. CJNC, FA-G, and BSSL wrote the manuscript. DRR and CP-S reviewed the manuscript.

Corresponding author

Correspondence to Clarisse Palma-Silva.

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Chaves, C.J.N., Aoki-Gonçalves, F., Leal, B.S.S. et al. Transferability of nuclear microsatellite markers to the atmospheric bromeliads Tillandsia recurvata and T. aeranthos (Bromeliaceae). Braz. J. Bot 41, 931–935 (2018). https://doi.org/10.1007/s40415-018-0494-4

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  • DOI: https://doi.org/10.1007/s40415-018-0494-4

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