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Auxin homeostasis and signaling alterations result in the aberrant phenotype in scl mutant of cotton (Gossypium hirsutum L.)

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Abstract

Leaves are the most important organs for photosynthesis. Many cotton leaf mutants (Gossypium spp.) have been reported and broadened the insights of leaf development. A natural occurring small, crinkled leaf (scl) mutant of upland cotton (Gossypium hirsutum L.) occurs in SY321 (wild type, WT), a high-yield upland cotton cultivar that is widely planted in China. The scl has a small and crinkled leaf phenotype. The shoot apical meristem (SAM) of scl did not form the essential typical layered structure, and the cell layers were arranged in a disordered manner. The free IAA concentration of shoot tips was significantly reduced in the scl according to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. Gene expression profiles showed that 432 genes were differentially expressed between scl and WT. The top 20 enriched pathways were analyzed using the Kyoto encyclopedia of genes and genomes (KEGG) database and four pathways, including flavonoid biosynthesis, photosynthesis, RNA transport, and carbon fixation in photosynthetic organisms, were considered as significantly enriched pathways. Furthermore, a number of differentially expressed genes related to auxin activity, including flavonoids biosynthesis, auxin perception and signal transduction pathway, auxin transport, and ubiquitin-mediated proteolysis pathways, were identified, and the altered expressions of these genes might be responsible for the aberrant phenotype of the scl.

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Acknowledgements

This work was supported by the Grants from the National Key Research and Development Program of China (2016YFD0100203), National Science Foundation in China (31501351), National Project of Modern Agricultural Industry Technology System in China (CARS-15-05), Taishan Scholars Program of Shandong Province (No. ts201511070), and The Youth Scientific Research Foundation of Shandong Academy of Agricultural Science (2015YQN04).

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  1. Jingxia Zhang and Yang Gao contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture, Cotton Research Center of Shandong Academy of Agricultural Sciences, Jinan, 250100, People’s Republic of China

    Jingxia Zhang, Yang Gao, Chuanyun Zhang, Guodong Liu, Yu Chen, Furong Wang & Jun Zhang

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  1. Jingxia Zhang
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  2. Yang Gao
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  6. Furong Wang
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  7. Jun Zhang
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Contributions

FW, JZ and JXZ designed the experiments, JXZ and YG carried out the lab experiments, CZ, GL and YC contributed to data analysis, FW, JZ and JXZ contributed to writing the paper.

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Correspondence to Furong Wang or Jun Zhang.

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Zhang, J., Gao, Y., Zhang, C. et al. Auxin homeostasis and signaling alterations result in the aberrant phenotype in scl mutant of cotton (Gossypium hirsutum L.). Braz. J. Bot 41, 775–784 (2018). https://doi.org/10.1007/s40415-018-0493-5

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