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Transferability of microsatellite markers in four species of Masdevallia (Orchidaceae, Pleurothallidinae) endemic to mountain cloud forests of the Bolivian Andes: a phylogenetic approach

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Abstract

Cross-amplification and transferability of ten microsatellite loci previously developed for Masdevallia solomonii Luer & R.Vasquéz were assessed in M. chaparensis T. Hashim, M. exquisita Luer & Hirtz, M. scandens Rolfe and M. yungasensis Hashimoto. The transferability rates and a phylogenetic analysis are presented here to show potential application for these markers. Cross-amplification was fully successful (100 %) in closely related species (M. yungasensis and M. chaparensis) and highly successful in M. exquisita and M. scandens (90 %). Average allele number/effective allele was 9.3/6.0 in M. chaparensis, 9.4/5.7 in M. exquisita, 5.8/3.2 in M. scandens and 6.2/3.9 in M. yungasensis. Deviations from Hardy–Weinberg equilibrium were observed in seven loci in M. chaparensis, five loci in M. exquisita, one locus in M. scandens, and two loci in M. yungasensis. The high transferability rates obtained indicate high potential for comparative population genetic studies in these species of great interest for conservation.

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Acknowledgments

We thank Paulo Ricardo M. Almeida for support in laboratory, Gabriela Villegas Alvarado, Beatriz Mamani Sánchez, Iván Jimenez Pérez and Saul Altamirano, for support in the field. Special thanks to Jorge Quezada Portugal of IBMB and the staff of LPB for logistic support. We thank Lucas Marinho Cardoso for helping us in figure edition. This work was funded by PRONEX (FAPESB/CNPq, Grant PNX0014/20090), the Productivity Scholarship from CNPq (PQ-1B) to C.v.d.B. and a scholarship from CNPq (PEC-PG 190107/09-0) to M.C.L.R.

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Authors and Affiliations

  1. Laboratório de Sistemática Molecular de Plantas, Universidade Estadual de Feira de Santana, Av. Transnordestina, s.n.,, Feira de Santana, BA, 44036-900, Brazil

    María Cristina Lopez-Roberts & Cássio van den Berg

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  1. María Cristina Lopez-Roberts
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  2. Cássio van den Berg
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Correspondence to María Cristina Lopez-Roberts.

Appendix

Appendix

Taxon; Voucher specimen, collection locale; Herbarium. Number of Genbank accession.

Masdevallia exquisita Luer & Hirtz JI4315, PN-ANMI Cotapata, Nor Yungas Bolivia; LPB. Genbank accession: KF479479.

Masdevallia scandens Rolfe JI5743, PN-ANMI Cotapata, Nor Yungas Bolivia; LPB. Genbank accession: KF479476.

Masdevallia solomonii Luer & Vasquez JI3742, PN-ANMI Cotapata, Nor Yungas Bolivia; LPB. Genbank accession: KF479477.

Masdevallia yungasensis Hashimoto subp. yungasensis JI3690, PN-ANMI Cotapata, Nor Yungas Bolivia; LPB. Genbank accession: KF479478.

Since Masdevallia chaparensis is a threatened species, we did not collect voucher specimen for the genotyped population. For phylogenetic analyses, we obtained the ITS sequences of M. chaparensis and Trisetella triglochin from GenBank (AF262797.1 and AF262807.1, respectively).

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Lopez-Roberts, M.C., van den Berg, C. Transferability of microsatellite markers in four species of Masdevallia (Orchidaceae, Pleurothallidinae) endemic to mountain cloud forests of the Bolivian Andes: a phylogenetic approach. Braz. J. Bot 39, 943–948 (2016). https://doi.org/10.1007/s40415-016-0264-0

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  • DOI: https://doi.org/10.1007/s40415-016-0264-0

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