In vitro inhibitory effect of two commercial probiotics on chromogenic actinomycetes

Abstract

Purpose

Black extrinsic discoloration is a common clinical and aesthetic problem. This study aims to evaluate the potential in vitro antagonistic activity of two commercial probiotics, Streptococcus salivarius M18 and Lactobacillus reuteri ProDentis, against microorganisms associated with black stains.

Methods

Streptococcus salivarius M18 and Lactobacillus reuteri were tested against Aggregatibacter actinomycetemcomitans and Actinomyces naeslundiiusing their cell-free fermentative broth in a planktonic growth inhibition test.

Results

Both probiotic cell-free supernatants showed the ability to reduce the pathogenic bacteria growth in a dose-dependent way. Streptococcus salivarius M18 showed a stronger antimicrobial activity than Lactobacillus reuteri ProDentis against the two indicator strains used. A. naeslundi was less susceptible to the probiotic activity of both S. salivarius and L. reuteri compared to A. actinomycetemcomitans.

Conclusions

The obtained results demonstrate a potent antagonistic ability of probiotics to reduce the growth of microorganisms associated with black tooth stains. Therefore, these strains could be evaluated for a therapeutic use against dental pigmentations.

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Acknowledgements

The Authors are grateful to Dr Veronica Cappa for the statistical support.

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Correspondence to E. Bardellini.

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This study did not need informed consent from the patients, being an in vitro study.

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Gobbi, E., De Francesco, M.A., Piccinelli, G. et al. In vitro inhibitory effect of two commercial probiotics on chromogenic actinomycetes. Eur Arch Paediatr Dent 21, 673–677 (2020). https://doi.org/10.1007/s40368-020-00512-2

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Keywords

  • Aggregatibacter actinomycetemcomitans
  • Actinomyces naeslundii
  • Streptococcus salivarius M18
  • Lactobacillus reuteri
  • Black stain