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Loop Mediated Isothermal Amplification: A Promising Tool for Screening Genetic Mutations

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Abstract

Mutation screening is elemental for clinical diagnosis and in determining therapeutic strategies. Nucleic acid-based techniques are considered to be the most accurate tools in genetic diagnosis. One such technique is loop-mediated isothermal amplification (LAMP) assay, which has seen tremendous applications in recent years. The advantages of the assay lie in its rapidity, efficiency, sensitivity, and cost. It works in isothermal conditions and amplifies the target gene using DNA polymerases that have strand displacement activity. To date, the assay has been widely used in different fields of research, including pathogen detection, crop development, and disease diagnosis. However, despite the potential, its application in mutation screening has been minimal. This review highlights the LAMP assay and its variants that have been developed for screening single-nucleotide polymorphisms and gene translocations in cancer.

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References

  1. Peake I. The polymerase chain reaction. J Clin Pathol. 1989;42:673.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Compton J. Nucleic acid sequence-based amplification. Nature. 1991;350:91–2.

    Article  CAS  PubMed  Google Scholar 

  3. Guatelli JC, Whitfield KM, Kwoh DY, Barringer KJ, Richman DD, Gingeras TR. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proc Natl Acad Sci USA. 1990;87:1874–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Lizardi PM, Huang X, Zhu Z, Bray-Ward P, Thomas DC, Ward DC. Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat Genet. 1998;19:225.

    Article  CAS  PubMed  Google Scholar 

  5. Walker GT, Fraiser MS, Schram JL, Little MC, Nadeau JG, Malinowski DP. Strand displacement amplification—an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 1992;20:1691–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Garibyan L, Avashia N. Research techniques made simple: polymerase chain reaction (PCR). J Invest Dermatol. 2013;133:1–4.

    Article  CAS  PubMed  Google Scholar 

  7. Sahoo PR, Sethy K, Mohapatra S, Panda D. Loop mediated isothermal amplification: an innovative gene amplification technique for animal diseases. Vet World. 2016;9:465.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Fakruddin MD. Loop mediated isothermal amplification (LAMP)–an alternative to polymerase chain reaction (PCR). Bangladesh Res Publ J. 2011;5:425–39.

    Google Scholar 

  9. Lucchi NW, Ljolje D, Silva-Flannery L, Udhayakumar V. Use of malachite green-loop mediated isothermal amplification for detection of Plasmodium spp. parasites. PLoS One. 2016;11:e0151437.

  10. Poon LL, Wong BW, Ma EH, Chan KH, Chow LM, Abeyewickreme W, Tangpukdee N, Yuen KY, Guan Y, Looareesuwan S, Peiris JM. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006;52:303–6.

    Article  CAS  PubMed  Google Scholar 

  11. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:e63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Bista BR, Ishwad C, Wadowsky RM, Manna P, Randhawa PS, Gupta G, Adhikari M, Tyagi R, Gasper G, Vats A. Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. J Clin Microbiol. 2007;45:1581–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Yoneyama T, Kiyohara T, Shimasaki N, Kobayashi G, Ota Y, Notomi T, Totsuka A, Wakita T. Rapid and real-time detection of hepatitis A virus by reverse transcription loop-mediated isothermal amplification assay. J Virol Methods. 2007;145:162–8.

    Article  CAS  PubMed  Google Scholar 

  14. Kundapur RR, Nema V. Loop-mediated isothermal amplification: beyond microbial identification. Cogent Biol. 2016;2:1137110.

    Article  CAS  Google Scholar 

  15. Fukuta S, Mizukami Y, Ishida A, Ueda J, Hasegawa M, Hayashi I, Hashimoto M, Kanbe M. Real-time loop-mediated isothermal amplification for the CaMV-35S promoter as a screening method for genetically modified organisms. Eur Food Res Technol. 2004;218:496–500.

    Article  CAS  Google Scholar 

  16. Lee D, La Mura M, Allnutt TR, Powell W. Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences. BMC Biotechnol. 2009;9:7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Liu M, Luo Y, Tao R, He R, Jiang K, Wang B, Wang L. Sensitive and rapid detection of genetic modified soybean (roundup ready) by loop-mediated isothermal amplification. Biosci Biotechnol Biochem. 2009;73:2365–9.

    Article  CAS  PubMed  Google Scholar 

  18. Guan X, Guo J, Shen P, Yang L, Zhang D. Visual and rapid detection of two genetically modified soybean events using loop-mediated isothermal amplification method. Food Anal Methods. 2010;3:313–20.

    Article  Google Scholar 

  19. Chen L, Guo J, Wang Q, Kai G, Yang L. Development of the visual loop-mediated isothermal amplification assays for seven genetically modified maize events and their application in practical samples analysis. J Agric Food Chem. 2011;59:5914–8.

    Article  CAS  PubMed  Google Scholar 

  20. Njiru ZK. Loop-mediated isothermal amplification technology: towards point of care diagnostics. PLoS Negl Trop Dis. 2012;6:e1572.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Mori Y, Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother. 2009;15:62–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002;16:223–9.

    Article  CAS  PubMed  Google Scholar 

  23. Sun WW, Sun Q, Yan LP, Zhang Q. The application of IS6110-baced loop-mediated isothermal amplification (LAMP) in the early diagnosis of tuberculous meningitis. Oncotarget. 2017;8:57537–42.

    PubMed  PubMed Central  Google Scholar 

  24. Saharan P, Dhingolia S, Khatri P, Duhan JS, Gahlawat S. Loop-mediated isothermal amplification (LAMP) based detection of bacteria: a review. Afr J Biotechnol. 2014;13:1920–8.

    Article  CAS  Google Scholar 

  25. Zhao Y, Chen F, Li Q, Wang L, Fan C. Isothermal amplification of nucleic acids. Chem Rev. 2015;115:12491–545.

    Article  CAS  PubMed  Google Scholar 

  26. Zhang X, Lowe SB, Gooding JJ. Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron. 2014;61:491–9.

    Article  CAS  PubMed  Google Scholar 

  27. Notomi T, Mori Y, Tomita N, Kanda H. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J Microbiol. 2015;53:1–5.

    Article  CAS  PubMed  Google Scholar 

  28. Biswal D. Advances in loop-mediated isothermal amplification (LAMP) technology and its necessity to detect helminth infections: an overview. Biomarkers J. 2016;2:1–5.

    Google Scholar 

  29. Li S, Wang Y, Li Y, Jiang J, Yu R, Xiang MS, Xia G. Loop-mediated isothermal amplification (LAMP): real-time methods for the detection of the survivin gene in cancer cells. Anal Methods. 2016;8:6277–83.

  30. Matsuzaki I, Iguchi H, Mikasa Y, Morishita H, Okuda K, Nakaguchi K, Mori Y, Iwahashi Y, Warigaya K, Fujimoto M, Kojima F. Novel application of loop-mediated isothermal amplification for rapid detection of gene translocation. Acta Histochem Cytochem. 2017;50:169–76.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Maeda J, Inoue M, Nakabayashi K, Otomo Y, Shintani Y, Ohta M, Okumura M, Matsuura N. Rapid diagnosis of lymph node metastasis in lung cancer with loop-mediated isothermal amplification assay using carcinoembryonic antigen–mRNA. Lung Cancer. 2009;65:324–7.

    Article  PubMed  Google Scholar 

  32. Liu Y, Zhao Y, Qin Y, Du X, Wang Q, Lyu J. A novel microfluidic device that integrates nucleic acid extraction, amplification, and detection to identify an EGFR mutation in lung cancer tissues. RSC Adv. 2016;6:13399–406.

    Article  CAS  Google Scholar 

  33. Nakamura N, Ito K, Takahashi M, Hashimoto K, Kawamoto M, Yamanaka M, Taniguchi A, Kamatani N, Gemma N. Detection of six single-nucleotide polymorphisms associated with rheumatoid arthritis by a loop-mediated isothermal amplification method and an electrochemical DNA chip. Anal Chem. 2007;79:9484–93.

    Article  CAS  PubMed  Google Scholar 

  34. Kuzuhara Y, Yonekawa T, Iwasaki M, Kadota T, Kanda H, Horigome T, Notomi T. Homogeneous assays for single-nucleotide polymorphism genotyping: exo-proofreading assay based on loop-mediated isothermal amplification. Yokohama Med J. 2005;56:9–16.

    CAS  Google Scholar 

  35. Horibe D, Ochiai T, Shimada H, Tomonaga T, Nomura F, Gun M, Tanizawa T, Hayashi H. Rapid detection of metastasis of gastric cancer using reverse transcription loop-mediated isothermal amplification. Int J Cancer. 2007;120:1063–9.

    Article  CAS  PubMed  Google Scholar 

  36. Yoneda A, Taniguchi K, Torashima Y, Susumu S, Kanetaka K, Kuroki T, Eguchi S. The detection of gastric cancer cells in intraoperative peritoneal lavage using the reverse transcription–loop-mediated isothermal amplification method. J Surg Res. 2014;187:e1–6.

    Article  CAS  PubMed  Google Scholar 

  37. Ikeda S, Takabe K, Inagaki M, Funakoshi N, Suzuki K. Detection of gene point mutation in paraffin sections using in situ loop-mediated isothermal amplification. Pathol Int. 2007;57:594–9.

    Article  CAS  PubMed  Google Scholar 

  38. Minnucci G, Amicarelli G, Salmoiraghi S, Spinelli O, Montalvo ML, Giussani U, Adlerstein D, Rambaldi A. A novel, highly sensitive and rapid allele-specific loop-mediated amplification assay for the detection of the JAK2V617F mutation in chronic myeloproliferative neoplasms. Haematologica. 2012;97:1394–400.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Carlos FF, Veigas B, Matias AS, Doria G, Flores O, Baptista PV. Allele specific LAMP-gold nanoparticle for characterization of single nucleotide polymorphisms. Biotechnol Rep. 2017;16:21–5.

    Article  Google Scholar 

  40. Zhang C, Yao Y, Zhu JL, Zhang SN, Zhang SS, Wei H, Hui WL, Cui YL. Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms. Sci Rep. 2016;6:26533.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Itonaga M, Matsuzaki I, Warigaya K, Tamura T, Shimizu Y, Fujimoto M, Kojima F, Ichinose M, Murata SI. Novel methodology for rapid detection of KRAS mutation using PNA-LNA mediated loop-mediated isothermal amplification. PLoS ONE. 2016;11:e0151654.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Raack EJ. A rapid colorimetric peptide nucleic acid loop-mediated isothermal amplification assay for the detection of the IDH1 mutation in glioblastoma. All NMU Master’s Theses. 2017. p. 158.

  43. Matsumoto N, Kumasaka A, Ando T, Komiyama K. Detection of EGFR gene mutation by mutation-oriented LAMP method. Anticancer Res. 2018;38:2093–9.

    Article  CAS  PubMed  Google Scholar 

  44. Fu Y, Duan X, Huang J, Huang L, Zhang L, Cheng W, Ding S, Min X. Detection of KRAS mutation via ligation-initiated LAMP reaction. Sci Rep. 2019;9:5955.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. LaBarre P, Hawkins KR, Gerlach J, Wilmoth J, Beddoe A, Singleton J, Boyle D, Weigl B. A simple, inexpensive device for nucleic acid amplification without electricity—toward instrument-free molecular diagnostics in low-resource settings. PLoS One. 2011;6:e19738.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Abdul-Ghani R, Al-Mekhlafi AM, Karanis P. Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test? Acta Trop. 2012;122:233–40.

    Article  CAS  PubMed  Google Scholar 

  47. Parida M, Sannarangaiah S, Dash PK, Rao PV, Morita K. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol. 2008;18:407–21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Neonakis IK, Spandidos DA, Petinaki E. Use of loop-mediated isothermal amplification of DNA for the rapid detection of Mycobacterium tuberculosis in clinical specimens. Eur J Clin Microbiol Infect Dis. 2011;30:937–42.

    Article  CAS  PubMed  Google Scholar 

  49. Inácio J, Flores O, Spencer-Martins I. Efficient identification of clinically relevant Candida yeast species by use of an assay combining panfungal loop-mediated isothermal DNA amplification with hybridization to species-specific oligonucleotide probes. J Clin Microbiol. 2008;46:713–20.

    Article  CAS  PubMed  Google Scholar 

  50. Wang L, Shi L, Alam MJ, Geng Y, Li L. Specific and rapid detection of foodborne Salmonella by loop-mediated isothermal amplification method. Food Res Int. 2008;41:69–74.

    Article  CAS  Google Scholar 

  51. Kaneko H, Kawana T, Fukushima E, Suzutani T. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods. 2007;70:499–501.

    Article  CAS  PubMed  Google Scholar 

  52. Kiddle G, Hardinge P, Buttigieg N, Gandelman O, Pereira C, McElgunn CJ, Rizzoli M, Jackson R, Appleton N, Moore C, Tisi LC. GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification suitable for field use. BMC Biotechnol. 2012;12:15.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Hill J, Beriwal S, Chandra I, Paul VK, Kapil A, Singh T, Wadowsky RM, Singh V, Goyal A, Jahnukainen T, Johnson JR. Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. J Clin Microbiol. 2008;46:2800–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Dugan L, Bearinger J, Hinckley A, Strout C, Souza B. Detection of Bacillus anthracis from spores and cells by loop-mediated isothermal amplification without sample preparation. J Microbiol Methods. 2012;90:280–4.

    Article  CAS  PubMed  Google Scholar 

  55. Tanner NA, Evans TC. Loop-mediated isothermal amplification for detection of nucleic acids. Curr Protoc Mol Biol. 2014;105:15-4.

    Article  Google Scholar 

  56. Goto M, Honda E, Ogura A, Nomoto A, Hanaki KI. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques. 2009;46:167–72.

    Article  CAS  PubMed  Google Scholar 

  57. Asiello PJ, Baeumner AJ. Miniaturized isothermal nucleic acid amplification, a review. Lab Chip. 2011;11:1420–30.

    Article  CAS  PubMed  Google Scholar 

  58. Sako Y, Nkouawa A, Yanagida T, Ito A. Loop-mediated isothermal amplification method for a differential identification of human Taenia tapeworms. Methods Mol Biol. 2013;1039:109–20.

    Article  CAS  PubMed  Google Scholar 

  59. Curtis KA, Rudolph DL, Nejad I, Singleton J, Beddoe A, Weigl B, LaBarre P, Owen SM. Isothermal amplification using a chemical heating device for point-of-care detection of HIV-1. PLoS One. 2012;7:e31432.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Mori Y, Kanda H, Notomi T. Loop-mediated isothermal amplification (LAMP): recent progress in research and development. J Infect Chemother. 2013;19:404–11.

    Article  CAS  PubMed  Google Scholar 

  61. Tanner NA, Zhang Y, Evans TC Jr. Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. Biotechniques. 2012;53:81–9.

    Article  CAS  PubMed  Google Scholar 

  62. Abe T, Segawa Y, Watanabe H, Yotoriyama T, Kai S, Yasuda A, Shimizu N, Tojo N. Point-of-care testing system enabling 30 min detection of influenza genes. Lab Chip. 2011;11:1166–7.

    Article  CAS  PubMed  Google Scholar 

  63. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001;289:150–4.

    Article  CAS  PubMed  Google Scholar 

  64. Kubota R, Vine BG, Alvarez AM, Jenkins DM. Detection of Ralstonia solanacearum by loop-mediated isothermal amplification. Phytopathology. 2008;98:1045–51.

    Article  CAS  PubMed  Google Scholar 

  65. Le TH, Nguyen NT, Truong NH, Van De N. Development of mitochondrial loop-mediated isothermal amplification for detection of the small liver fluke Opisthorchis viverrini (Opisthorchiidae; Trematoda; Platyhelminthes). J Clin Microbiol. 2012;50:1178–84.

    Article  PubMed  PubMed Central  Google Scholar 

  66. Liu W, Huang S, Liu N, Dong D, Yang Z, Tang Y, Ma W, He X, Ao D, Xu Y, Zou D. Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay. Sci Rep. 2017;7:40125.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  67. Le Roux CA, Kubo T, Grobbelaar AA, van Vuren PJ, Weyer J, Nel LH, Swanepoel R, Morita K, Paweska JT. Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid detection of Rift Valley fever virus in clinical specimens. J Clin Microbiol. 2009;47:645–51.

    Article  CAS  PubMed  Google Scholar 

  68. Mori Y, Kitao M, Tomita N, Notomi T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods. 2004;59:145–57.

    Article  CAS  PubMed  Google Scholar 

  69. Wang F, Jiang L, Ge B. Loop-mediated isothermal amplification assays for detecting Shiga toxin-producing Escherichia coli in ground beef and human stools. J Clin Microbiol. 2012;50:91–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  70. Denschlag C, Vogel RF, Niessen L. Hyd5 gene based analysis of cereals and malt for gushing-inducing Fusarium spp. by real-time LAMP using fluorescence and turbidity measurements. Int J Food Microbiol. 2013;162:245–51.

  71. Higgins O, Clancy E, Cormican M, Boo T, Cunney R, Smith T. Evaluation of an internally controlled multiplex Tth endonuclease cleavage Loop-mediated isothermal amplification (TEC-LAMP) assay for the detection of bacterial Meningitis pathogens. Int J Mol Sci. 2018;19:524.

    Article  CAS  PubMed Central  Google Scholar 

  72. Gadkar VJ, Goldfarb DM, Gantt S, Tilley PA. Real-time detection and monitoring of loop- mediated amplification (LAMP) reaction using self-quenching and de-quenching fluorogenic probes. Sci Rep. 2018;8:5548.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Jiang YS, Bhadra S, Li B, Wu YR, Milligan JN, Ellington AD. Robust strand exchange reactions for the sequence-specific, real-time detection of nucleic acid amplicons. Anal Chem. 2015;87:3314–20.

    Article  CAS  PubMed  Google Scholar 

  74. Chen X, Sullivan PF. Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput. Pharmacogenomics J. 2003;3:77–96.

    Article  CAS  PubMed  Google Scholar 

  75. Sachidanandam R, Weissman D, Schmidt SC, Kakol JM, Stein LD, Marth G, Sherry S, Mullikin JC, Mortimore BJ, Willey DL, Hunt SE, Cole CG, Coggill PC, Rice CM, Ning Z, Rogers J, Bentley DR, Kwok PY, Mardis ER, Yeh RT, Schultz B, Cook L, Davenport R, Dante M, Fulton L, Hillier L, Waterston RH, McPherson JD, Gilman B, Schaffner S, Van Etten WJ, Reich D, Higgins J, Daly MJ, Blumenstiel B, Baldwin J, Stange-Thomann N, Zody MC, Linton L, Lander ES, Altshuler D. A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms. Nature. 2001;409:928–33.

  76. Singleton J, Osborn JL, Lillis L, Hawkins K, Guelig D, Price W, Johns R, Ebels K, Boyle D, Weigl B, LaBarre P. Electricity-free amplification and detection for molecular point-of-care diagnosis of HIV-1. PLoS One. 2014;9:e113693.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. Gurrala R, Lang Z, Shepherd L, Davidson D, Harrison E, McClure M, Kaye S, Toumazou C, Cooke GS. Novel pH sensing semiconductor for point-of-care detection of HIV-1 viremia. Sci Rep. 2016;6:36000.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. Park BH, Oh SJ, Jung JH, Choi G, Seo JH, Lee EY, Seo TS. An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics. Biosens Bioelectron. 2017;91:334–40.

    Article  CAS  PubMed  Google Scholar 

  79. Song J, Mauk MG, Hackett BA, Cherry S, Bau HH, Liu C. Instrument-free point-of-care molecular detection of Zika virus. Anal Chem. 2016;88:7289–94.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  80. Wan L, Chen T, Gao J, Dong C, Wong AH, Jia Y, Mak PI, Deng CX, Martins RP. A digital microfluidic system for loop-mediated isothermal amplification and sequence specific pathogen detection. Sci Rep. 2017;7:14586.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  81. Anupama KP, Chakraborty A, Karunasagar I, Karunasagar I, Maiti B. Loop-mediated isothermal amplification assay as a point-of-care diagnostic tool for Vibrio parahaemolyticus: recent developments and improvements. Expert Rev Mol Diagn. 2019;19:229–39.

    Article  CAS  PubMed  Google Scholar 

  82. Erlichster M, Tye-Din JA, Varney MD, Skafidas E, Kwan P. Rapid, loop-mediated isothermal amplification detection of celiac disease risk alleles. J Mol Diagn. 2018;20:307–15.

    Article  CAS  PubMed  Google Scholar 

  83. Sharma G, Tewari R, Dhatwalia SK, Yadav R, Behera D, Sethi S. A loop-mediated isothermal amplification assay for the diagnosis of pulmonary tuberculosis. Lett Appl Microbiol. 2019;68:219–25.

    Article  CAS  PubMed  Google Scholar 

  84. Seki M, Kilgore PE, Kim EJ, Ohnishi M, Hayakawa S, Kim DW. Loop-mediated isothermal amplification methods for diagnosis of bacterial meningitis. Front Pediatr. 2018;6:57.

    Article  PubMed  PubMed Central  Google Scholar 

  85. Yang Y, Qin X, Wang G, Jin J, Shang Y, Zhang Z. Development of an isothermal amplification-based assay for rapid visual detection of an Orf virus. Virol J. 2016;13:46.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  86. Takayama I, Nakauchi M, Takahashi H, Oba K, Semba S, Kaida A, Kubo H, Saito S, Nagata S, Odagiri T, Kageyama T. Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assay with quenching primer for influenza virus and respiratory syncytial virus. J Virol Methods. 2019;267:53–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  87. Cao Y, Wang L, Duan L, Li J, Ma J, Xie S, Shi L, Li H. Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis. Sci Rep. 2017;7:13394.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  88. Zhang SY, Dai DJ, Wang HD, Zhang CQ. One-step loop-mediated isothermal amplification (LAMP) for the rapid and sensitive detection of Fusarium fujikuroi in bakanae disease through NRPS31, an important gene in the gibberellic acid bio-synthesis. Sci Rep. 2019;9:3726.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  89. Chakraborty G, Karunasagar I, Chakraborty A. Loop-mediated isothermal amplification (LAMP): a rapid molecular diagnosis technique for detection of human pathogens. Nitte Univ J Health Sci. 2017;7:42–8.

    Google Scholar 

  90. Mohon AN, Menard D, Alam MS, Perera K, Pillai DR. A novel SNP-LAMP assay for detection of artemisinin-resistant Plasmodium falciparum malaria. Open Forum Infect Dis. 2018;5:ofy011.

  91. Tambo M, Mwinga M, Mumbengegwi DR. Loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) as quality assurance tools for rapid diagnostic test (RDT) malaria diagnosis in northern Namibia. PLoS One. 2018;13:e0206848.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  92. Panek J, Frąc M. Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species. Sci Rep. 2019;9:5846.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  93. Kumar Y, Bansal S, Jaiswal P. Loop-mediated isothermal amplification (LAMP): a rapid and sensitive tool for quality assessment of meat products. Compr Rev Food Sci Food Saf. 2017;16:1359–78.

    Article  CAS  PubMed  Google Scholar 

  94. Yamanaka ES, Tortajada-Genaro LA, Pastor N, Maquieira Á. Polymorphism genotyping based on loop-mediated isothermal amplification and smartphone detection. Biosens Bioelectron. 2018;109:177–83.

    Article  CAS  PubMed  Google Scholar 

  95. Lin X, Huang X, Urmann K, Xie X, Hoffmann MR. Digital loop-mediated isothermal amplification on a commercial membrane. ACS Sens. 2019;4:242–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The support by Nitte (Deemed to the University) in the form of a PhD fellowship to the first author is gratefully acknowledged.

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  1. Division of Molecular Genetics and Cancer, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangaluru, 575018, India

    Arjuna Srividya, Anirban Chakraborty & Gunimala Chakraborty

  2. Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangaluru, 575018, India

    Biswajit Maiti

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  1. Arjuna Srividya
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Correspondence to Gunimala Chakraborty.

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The third author acknowledges Nitte (Deemed to be University), Mangaluru, India, for the financial support provided through an intramural research project (NUFR3/2016/05-04). In addition, this paper was partially supported by the Department of Biotechnology, Government of India through a project (BT/PR12230/AAQ/3/698/2014) awarded to the second author.

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The authors (AS, BM, AC, and GC) have no conflict of interest to declare.

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Srividya, A., Maiti, B., Chakraborty, A. et al. Loop Mediated Isothermal Amplification: A Promising Tool for Screening Genetic Mutations. Mol Diagn Ther 23, 723–733 (2019). https://doi.org/10.1007/s40291-019-00422-0

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