Electrochemical Detection of Superoxide Anion Released by Living Cells by Manganese(III) Tetraphenyl Porphine as Superoxide Dismutase Mimic

Chemical Research in Chinese Universities volume 36, pages774–780(2020)Cite this article

Abstract

Superoxide anion, one of the most active reactive oxygen species, is associated with the development of many diseases. So monitoring superoxide anion in living cells is of great significance for the pathological research of many diseases. In this work, a new non-enzymatic sensor for the detection of superoxide anion(O•−2 ) was developed, which was fabricated by the nanocomposites composed of manganese(III) tetraphenyl porphine(MnTPP) as superoxide dismutase mimic and electrochemical reduced graphene oxide(ERGO) as electrode support material to modify the glassy carbon electrode(GCE). The electrochemical behavior of the fabricated electrode(MnTPP/ERGO/GCE) was performed by electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV), which revealed that MnTPP/ERGO/GCE possessed good catalytic ability to the electrochemical reduction of O•−2 . The MnTPP/ERGO/GCE showed excellent electroanalysis performance towards O•−2 using the technique of differential pulse voltammetry(DPV) with a linear relationship in the range of 0.2–110.0 µmol/L, a sensitivity of 445 µA·L·mmol−1·cm−2 and a detection limit of 0.039 µmol/L(S/N=3). The real-time monitoring of O•−2 from MCF-7 breast cancer cells stimulated by zymosan was realized in this work, which indicates that the MnTPP/ERGO/GCE hold potential application for electrochemical quantification of superoxide anions in biological applications.

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Affiliations

  1. Department of Chemistry, School of Sciences, Hebei University of Science and Technology, Shijiazhuang, 050018, P. R. China

    Min Cui, Jujie Ren, Xiaofang Wen, Na Li, Yifei Xing, Cong Zhang & Yuanyuan Han

  2. Department of Medical Chemistry, Hebei Medical University, Shijiazhuang, 050017, P. R. China

    Xueping Ji

Authors
  1. Min Cui
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  2. Jujie Ren
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  3. Xiaofang Wen
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  4. Na Li
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  5. Yifei Xing
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  6. Cong Zhang
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  7. Yuanyuan Han
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  8. Xueping Ji
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Corresponding authors

Correspondence to Jujie Ren or Xueping Ji.

Additional information

Supported by the National Natural Science Foundation of China(No.81872669) and the Scientific Research Projects of the Department of Education of Hebei Province, China(Nos.ZD2018037, QN2019140).

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Cui, M., Ren, J., Wen, X. et al. Electrochemical Detection of Superoxide Anion Released by Living Cells by Manganese(III) Tetraphenyl Porphine as Superoxide Dismutase Mimic. Chem. Res. Chin. Univ. 36, 774–780 (2020). https://doi.org/10.1007/s40242-019-0006-5

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Keywords

  • Superoxide anion
  • Electrochemical sensor
  • Manganese(III) tetraphenyl porphine
  • Electrochemical reduced graphene oxide
  • MCF-7 breast cancer cell