Abstract
Glutathione(GSH), as the most abundant intracellular biothiol, plays an important role in the redox homeostasis of the organism. Abnormal concentrations of GSH in cells may lead to many malignant diseases, such as cancer, liver damage and neurodegenerative diseases. It is urgent to develop effective methods to detect GSH in living organisms. In this work, a new two-photon ratiometric fluorescent probe Co-GSH based on the coumarinchalcone dye platform was judiciously developed. Based on the Michael-addition reaction, Co-GSH was able to identify GSH with high selectivity and sensitivity. Furthermore, assisted by laser-scanning confocal microscopy, Co-GSH could specifically response GSH over the other biothiols, including Cys and Hcy, in living HeLa cells by using one- and two-photon modes.
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Miller G. L., J. Am. Chem. Soc., 1960, 82(16), 4439
Hyman L. M., Franz K. J., Coord. Chem. Rev., 2012, 256(20), 2333
Meister A., Anderson M. E., Ann. Rev. Biochem., 1983, 52(6), 711
Townsend D. M., Tew K. D., Tapiero H., Biomed. Pharmacother., 2003, 57(4), 145
Carvalho F. D., Remião F., Valet P., Timbrell J. A., Bastos M. L., Ferreira M. A., Biomed. Chromatogr., 1994, 8(3), 134
Raoof J. B., Ojani R., Baghayeri M., Sens. Actuators B, 2009, 143(1), 261
Rellán-Álvarez R., Hernández L. E., Abadía J., Álvarez-Fernández A., Anal. Biochem., 2006, 356(2), 254
Lakowicz J. R., Principles of Fluorescence Spectroscopy, Springer US, Boston, 2006, 607
Kim H. M., Cho B. R., Chem. Rev., 2015, 115(11), 5014
Carrone G., Etchenique R., Anal. Chem., 2015, 87(8), 4363
Niu L., Guan Y., Chen Y., Wu L., Tung C., Yang Q., Chem. Commun., 2013, 49(13), 1294
Lim S. Y., Na M. J., Kim H. J., Sens. Actuators B, 2013, 185(8), 720
Hong K. H., Kim D. I., Kwon H., Kim H. J., RSC Adv., 2014, 4(2), 978
Wang S., Wu Q., Wang H., Zheng X., Shen S., Zhang Y., Miao J., Zhao B., Biosens. Bioelectron., 2014, 55(9), 386
Hou X., Guo X., Chen B., Liu C., Gao F., Zhao J., Wang J., Sens. Actuators, B, 2015, 209, 838
Chen C., Liu W., Xu C., Liu W., Biosens. Bioelectron, 2015, 71, 68
Liu M., Jiang Q., Lu Z., Huang Y., Tan Y., Jiang Q., Luminescence, 2015, 30(8), 1395
Qi F., Liu X., Yang L., Chen W., Song X., Tetrahedron, 2016, 72(44), 6909
Li J., Yin C., Chao J., Huo F., Anal. Methods, 2016, 8(37), 6748
Wang Y., Zhu M., Jiang E., Hua R., Li Q., Sci. Rep., 2017, 7(1), 4377
Li Y., Wang K., Liu B., Lu X., Li M., Ji L., Mao Z., Sens. Actuators, B, 2018, 255, 193
Wang F., Feng C., Lu L., Xu Z., Zhang W., Talanta, 2018, 169, 149
Li J., Kwon Y., Chung K. S., Chang S. L., Lee D., Yin C., Theranos-tics, 2018, 8(5), 1411
Hu Q., Yu C., Xia X., Zeng F., Wu S., Biosens. Bioelectron., 2016, 81, 341
Cheng X., Jia H., Feng. J., J. Mater. Chem. B, 2013, 1(33), 4110
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Supported by the National Natural Science Foundation of China(Nos.21472067, 21672083), the Taishan Scholar Foundation, China(No.TS20151104 1) and the Startup Fund of University of Jinan, China(No.309-10004).
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Xu, G., Tang, Y. & Lin, W. Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells. Chem. Res. Chin. Univ. 34, 523–527 (2018). https://doi.org/10.1007/s40242-018-8089-y
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DOI: https://doi.org/10.1007/s40242-018-8089-y