Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro
- 20 Downloads
In this paper, antibody-modified silica nanoparticles were successfully synthesized, and their average di-ameter was (109±9) nm. These particles were mixed with cell extracts to target a protein, then, an antibody labeled with fluorescein isothiocyanate(FITC) was added to form FITC-labeled nanoparticle complexes and the product was analyzed using flow cytometry. The results confirm that the intracellular proteins and biomarkers were precisely and sensitivity detected by the novel method in vitro. The FITC intensity of Akt antibody-conjugated particles was in-creased ≥10-fold compared with that of control samples in MCF-7 cells. Furthermore, it can also simultaneously measure several proteins when modified with different antibodies.
KeywordsSilica nanoparticle Flow cytometry Intracellular protein Biomarker
Unable to display preview. Download preview PDF.
- Hutchinson J., Jin J., Cardiff R. D., Woodgett J. R., Muller W. J., Mol. Cell Biol., 2001, 21, 2203Google Scholar
- Jin H., Wang X., Xin T., Gao P., Lin J. M., Liang S., Chin. Sci. Bull., 2008, 53(19), 2958Google Scholar
- Zhang H. P., Han B., Jia Z. Z., Ding R. B., Xu B., Xu W. Q., Fan Z. M., Chem. J. Chinese Universities, 2017, 38(5), 860Google Scholar
- Herzenberg L. A., Parks D., Sahaf B., Perez O., Roederer M., Herzenberg L. A., Clin. Chem., 2002, 48, 1819Google Scholar
- Lukacs S., Szabo N., Woodhams S., Case Rep. Urol., 2012, 2012, 724940Google Scholar
- Ren H. T., Zhao Y. H., Guan H. Z., Li X. G., Shao Y. Q., Chen J. H., Chen L., Chin. J. Neuroimmunol and Neurol., 2011, 18(5), 332Google Scholar
- Ma Z. X., Guan Y. P., Zhang X. X., Wang J., Wang B. C., Rong X. D., Hu H., Chin. J. Cancer Biother., 2015, 22(4), 500Google Scholar