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Improving Catalytic Performance of Burkholderiacepacia Lipase by Chemical Modification with Functional Ionic Liquids

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Abstract

Various imidazolium and choline-based functional ionic liquids(ILs) comprising different cations and anions were grafted onto Burkholderiacepacia lipase(BCL) through surface amino acids coupling. The catalytic ac-tivity, thermostability, organic solvent tolerance and adaptability to temperature and pH changes of the modified BCL were then evaluated in olive oil hydrolysis reaction. The results showed that different combinations of cations and anions in ILs had important influence on the catalytic performance of the modified lipases. BCL modified with IL [Choline][H2PO4] was the most improved lipase, in which increases by 1.2 folds in relative activity, 2.5 folds in typi-cal proton solvent(10% methanol, volume fraction), and 1.4 folds in thermostability(after incubation at 70 °C for 2 h) were achieved in relative toits native form. BCL modified with [HOOCEPEG350IM][BF4] had higher optimal tempe- rature and pH, and better thermosability compared with the native and other modified BCLs. The conformational changes of BCLs were also confirmed by fluorescence spectroscopy and circular dichroism spectroscopy.

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 210009, P. R. China

    Chao Xu, Xuhan Yin, Chuan Zhang, Hongyue Chen, He Huang & Yi Hu

Authors
  1. Chao Xu
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  2. Xuhan Yin
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  3. Chuan Zhang
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  4. Hongyue Chen
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  5. He Huang
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  6. Yi Hu
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Corresponding author

Correspondence to Yi Hu.

Additional information

Supported by the National Natural Science Foundation of China(No.21676143), the Self-owned Research Project from Key Laboratory of Material-Oriented Chemical Engineering, China(No.ZK201603), the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture, China and Qing Lan Project of Jiangsu Province, China.

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Xu, C., Yin, X., Zhang, C. et al. Improving Catalytic Performance of Burkholderiacepacia Lipase by Chemical Modification with Functional Ionic Liquids. Chem. Res. Chin. Univ. 34, 279–284 (2018). https://doi.org/10.1007/s40242-018-7246-7

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  • DOI: https://doi.org/10.1007/s40242-018-7246-7

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