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Biohydrogen production under hyper salinity stress by an anaerobic sequencing batch reactor with mixed culture

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Abstract

Background

This study investigated the effect of organic loading rate (OLR) and NaCl concentration on biohydrogen production by preheated anaerobic sludge in a lab scale anaerobic sequencing batch reactor (ASBR) fed with glucose during long time operation.

Methods

During ASBR operation, the OLR was increased in steps from 0.5 to 5 g glucose/L.d and NaCl addition started at an OLR of 5 g glucose/L.d, to obtain NaCl concentrations in the reactor in the range of 0.5–30 g/L.

Results

With an increasing OLR from 0.5 to 5 g glucose/L.d, the biohydrogen yield increased and reached 0.8 ± 0.4 mol H2/mol glucose at an OLR of 5 g glucose/L.d. A NaCl concentration of 0.5 g/L resulted in a higher yield of biohydrogen (1.1 ± 0.2 mol H2/mol glucose). Concentrations above 0.5 g/L NaCl led to decreasing biohydrogen yield and the lowest yield (0.3 ± 0.1 mol H2/mol glucose) was obtained at 30 g/L of NaCl. The mass balance errors for C, H, and O in all constructed stoichiometric reactions were below 5%.

Conclusions

The modified Monod model indicated that r (H2)max and Ccrit values were 23.3 mL H2/g VSS/h and 119.9 g/L, respectively. Additionally, ASBR operation at high concentrations of NaCl shifted the metabolic pathway from acidogenic toward solventogenic.

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Abbreviations

C :

Added NaCl concentration (g/L)

C crit :

Critical NaCl concentration by H2 production ceases (g/L)

C I,50 :

NaCl concentration with repress 50% of relative activity (g/L)

k c :

Specific growth rate (1/h).

K s :

Apparent half velocity constant for the substrate (g/L)

n :

Inhibition degree

r(H 2 ) :

H2 production rate (mL H2/g VSS/h)

r(H 2 ) max :

Maximum H2 production rate (mL H2/g VSS/h)

S:

Substrate concentration (g/L)

SHPR:

Specific biohydrogen production rate (L H2/g VSS.d)

ν :

Specific substrate degradation rate (g/gVSS.h)

νm:

Maximum specific substrate degradation rate (g/gVSS.h)

X :

Microbial concentration (gVSS/L)

X 0 :

Initial microbial concentration (g VSS/L)

X max :

Maximum microbial concentration (g/L)

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Acknowledgements

The authors would like to thank the financial support of Isfahan University of Medical Sciences (IUMS) under project #394341.

Funding

This paper is issued from the thesis of Ensiyeh Taheri and Isfahan University of Medical Sciences (IUMS) was financial support.

Author information

Authors and Affiliations

  1. Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

    Ensiyeh Taheri, Mohammad Mehdi Amin, Ali Fatehizadeh, Hamidreza Pourzamani & Bijan Bina

  2. Student Research Committee, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

    Ensiyeh Taheri

  3. Environment Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

    Ensiyeh Taheri, Mohammad Mehdi Amin, Ali Fatehizadeh, Hamidreza Pourzamani & Bijan Bina

  4. Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, 2628, CN, Delft, The Netherlands

    Henri Spanjers

Authors
  1. Ensiyeh Taheri
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  2. Mohammad Mehdi Amin
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Contributions

Bijan Bina supervised the study, Ensiyeh Taheri was the main investigator, collected the data, Mohammad Mehdi Amin and Hamidreza Pourzamani were advisor the study, Ensiyeh Taheri, Ali Fatehizadeh, and Henri Spanjers drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bijan Bina.

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The authors declare that they have no competing interest.

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Taheri, E., Amin, M.M., Fatehizadeh, A. et al. Biohydrogen production under hyper salinity stress by an anaerobic sequencing batch reactor with mixed culture. J Environ Health Sci Engineer 16, 159–170 (2018). https://doi.org/10.1007/s40201-018-0304-8

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