Additive manufacturing of hydroxyapatite bioceramic scaffolds: Dispersion, digital light processing, sintering, mechanical properties, and biocompatibility


Hydroxyapatite (HA) bioceramic scaffolds were fabricated by using digital light processing (DLP) based additive manufacturing. Key issues on the HA bioceramic scaffolds, including dispersion, DLP fabrication, sintering, mechanical properties, and biocompatibility were discussed in detail. Firstly, the effects of dispersant dosage, solid loading, and sintering temperature were studied. The optimal dispersant dosage, solid loading, and sintering temperature were 2 wt%, 50 vol%, and 1250 °C, respectively. Then, the mechanical properties and biocompatibility of the HA bioceramic scaffolds were investigated. The DLP-prepared porous HA bioceramic scaffold was found to exhibit excellent mechanical properties and degradation behavior. From this study, DLP technique shows good potential for manufacturing HA bioceramic scaffolds.


  1. [1]

    Fu SY, Zhu M, Zhu YF. Organosilicon polymer-derived ceramics: An overview. J Adv Ceram 2019, 8: 457–478.

    CAS  Article  Google Scholar 

  2. [2]

    Wu Z, Zhou ZR, Hong YL. Isotropic freeze casting of through-porous hydroxyapatite ceramics. J Adv Ceram 2019, 8: 256–264.

    CAS  Article  Google Scholar 

  3. [3]

    Witek L, Shi Y, Smay J. Controlling calcium and phosphate ion release of 3D printed bioactive ceramic scaffolds: An in vitro study. J Adv Ceram 2017, 6: 157–164.

    CAS  Article  Google Scholar 

  4. [4]

    Shen TT, Yang WH, Shen XK, et al. Polydopamine-assisted hydroxyapatite and lactoferrin multilayer on titanium for regulating bone balance and enhancing antibacterial property. ACS Biomater Sci Eng 2018, 4: 3211–3223.

    CAS  Article  Google Scholar 

  5. [5]

    Ramay HR, Zhang MQ. Preparation of porous hydr-oxyapatite scaffolds by combination of the gel-casting and polymer sponge methods. Biomaterials 2003, 24: 3293–3302.

    CAS  Article  Google Scholar 

  6. [6]

    Lee EJ, Koh YH, Yoon BH, et al. Highly porous hydroxyapatite bioceramics with interconnected pore channels using camphene-based freeze casting. Mater Lett 2007, 61: 2270–2273.

    CAS  Article  Google Scholar 

  7. [7]

    Yang T, Lee JM, Yoon SY, et al. Hydroxyapatite scaffolds processed using a TBA-based freeze-gel casting/polymer sponge technique. J Mater Sci: Mater Med 2010, 21: 1495–1502.

    CAS  Google Scholar 

  8. [8]

    Yan S, Huang YF, Zhao DK, et al. 3D printing of nano-scale Al2O3-ZrO2 eutectic ceramic: Principle analysis and process optimization of pores. Addit Manuf 2019, 28: 120–126.

    CAS  Google Scholar 

  9. [9]

    Chen ZW, Li ZY, Li JJ, et al. 3D printing of ceramics: A review. J Eur Ceram Soc 2019, 39: 661–687.

    CAS  Article  Google Scholar 

  10. [10]

    Cheng ZL, Ye F, Liu YS, et al. Mechanical and dielectric properties of porous and wave-transparent Si3N4-Si3N4 composite ceramics fabricated by 3D printing combined with chemical vapor infiltration. J Adv Ceram 2019, 8: 399–407.

    CAS  Article  Google Scholar 

  11. [11]

    Du XY, Fu SY, Zhu YF. 3D printing of ceramic-based scaffolds for bone tissue engineering: An overview. J Mater Chem B 2018, 6: 4397–4412.

    CAS  Article  Google Scholar 

  12. [12]

    Tan KH, Chua CK, Leong KF, et al. Scaffold development using selective laser sintering of polyetheretherketone-hydroxyapatite biocomposite blends. Biomaterials 2003, 24: 3115–3123.

    CAS  Article  Google Scholar 

  13. [13]

    Hao L, Dadbakhsh S, Seaman O, et al. Selective laser melting of a stainless steel and hydroxyapatite composite for load-bearing implant development. J Mater Process Technol 2009, 209: 5793–5801.

    CAS  Article  Google Scholar 

  14. [14]

    Xu N, Ye XJ, Wei DX, et al. 3D artificial bones for bone repair prepared by computed tomography-guided fused deposition modeling for bone repair. ACS Appl Mater Interfaces 2014, 6: 14952–14963.

    CAS  Article  Google Scholar 

  15. [15]

    Wei QH, Wang YN, Chai WH, et al. Molecular dynamics simulation and experimental study of the bonding properties of polymer binders in 3D powder printed hydroxyapatite bioceramic bone scaffolds. Ceram Int 2017, 43: 13702–13709.

    CAS  Article  Google Scholar 

  16. [16]

    Vorndran E, Moseke C, Gbureck U. 3D printing of ceramic implants. MRS Bull 2015, 40: 127–136.

    CAS  Article  Google Scholar 

  17. [17]

    Brunello G, Sivolella S, Meneghello R, et al. Powder-based 3D printing for bone tissue engineering. Biotechnol Adv 2016, 34: 740–753.

    CAS  Article  Google Scholar 

  18. [18]

    Fu S, Hu HR, Chen JJ, et al. Silicone resin derived larnite/C scaffolds via 3D printing for potential tumor therapy and bone regeneration. Chem Eng J 2020, 382: 122928.

    Article  CAS  Google Scholar 

  19. [19]

    Fu S, Yu B, Ding HF, et al. Zirconia incorporation in 3D printed β-Ca2SiO4 scaffolds on their physicochemical and biological property. J Inorg Mater 2019, 34: 444.

    Article  Google Scholar 

  20. [20]

    Du XY, Wei DX, Huang L, et al. 3D printing of mesoporous bioactive glass/silk fibroin composite scaffolds for bone tissue engineering. Mater Sci Eng: C 2019, 103: 109731.

    CAS  Article  Google Scholar 

  21. [21]

    Shao H, He JZ, Lin T, et al. 3D gel-printing of hydroxyapatite scaffold for bone tissue engineering. Ceram Int 2019, 45: 1163–1170.

    CAS  Article  Google Scholar 

  22. [22]

    Sun L, Parker ST, Syoji D, et al. Direct-write assembly of 3D silk/hydroxyapatite scaffolds for bone Co-cultures. Adv Healthc Mater 2012, 1: 729–735.

    CAS  Article  Google Scholar 

  23. [23]

    Shao HF, Yang XY, He Y, et al. Bioactive glass-reinforced bioceramic ink writing scaffolds: Sintering, microstructure and mechanical behavior. Biofabrication 2015, 7: 035010.

    Article  CAS  Google Scholar 

  24. [24]

    Simon JL, Michna S, Lewis JA, et al. In vivo bone response to 3D periodic hydroxyapatite scaffolds assembled by direct ink writing. J Biomed Mater Res 2007, 83A: 747–758.

    CAS  Article  Google Scholar 

  25. [25]

    Ronca A, Ambrosio L, Grijpma DW. Preparation of designed poly(d,l-lactide)/nanosized hydroxyapatite composite structures by stereolithography. Acta Biomater 2013, 9: 5989–5996.

    CAS  Article  Google Scholar 

  26. [26]

    Skoog SA, Goering PL, Narayan RJ. Stereolithography in tissue engineering. J Mater Sci: Mater Med 2014, 25: 845–856.

    CAS  Google Scholar 

  27. [27]

    Wang Z, Huang CZ, Wang J, et al. Development of a novel aqueous hydroxyapatite suspension for stereolithography applied to bone tissue engineering. Ceram Int 2019, 45: 3902–3909.

    CAS  Article  Google Scholar 

  28. [28]

    Lasgorceix M, Champion E, Chartier T. Shaping by microstereolithography and sintering of macro-micro-porous silicon substituted hydroxyapatite. J Eur Ceram Soc 2016, 36: 1091–1101.

    CAS  Article  Google Scholar 

  29. [29]

    Chen QH, Zou B, Lai QG, et al. A study on biosafety of HAP ceramic prepared by SLA-3D printing technology directly. J Mech Behav Biomed Mater 2019, 98: 327–335.

    CAS  Article  Google Scholar 

  30. [30]

    Putlyaev VI, Evdokimov PV, Safronova TV, et al. Fabrication of osteoconductive Ca3-xM2x(PO4)2 (M = Na, K) calcium phosphate bioceramics by stereolithographic 3D printing. Inorg Mater 2017, 53: 529–535.

    CAS  Article  Google Scholar 

  31. [31]

    Wang M, Xie C, He RJ, et al. Polymer-derived silicon nitride ceramics by digital light processing based additive manufacturing. J Am Ceram Soc 2019, 102: 5117–5126.

    CAS  Article  Google Scholar 

  32. [32]

    Liu ZB, Liang HX, Shi TS, et al. Additive manufacturing of hydroxyapatite bone scaffolds via digital light processing and in vitro compatibility. Ceram Int 2019, 45: 11079–11086.

    CAS  Article  Google Scholar 

  33. [33]

    Zeng Y, Yan YZ, Yan HF, et al. 3D printing of hydroxyapatite scaffolds with good mechanical and biocompatible properties by digital light processing. J Mater Sci 2018, 53: 6291–6301.

    CAS  Article  Google Scholar 

  34. [34]

    Lee YH, Lee JB, Maeng WY, et al. Photocurable ceramic slurry using solid camphor as novel diluent for conventional digital light processing (DLP) process. J Eur Ceram Soc 2019, 39: 4358–4365.

    CAS  Article  Google Scholar 

  35. [35]

    He RX, Liu W, Wu ZW, et al. Fabrication of complex-shaped zirconia ceramic parts via a DLP- stereoli-thography-based 3D printing method. Ceram Int 2018, 44: 3412–3416.

    CAS  Article  Google Scholar 

  36. [36]

    Karalekas D, Aggelopoulos A. Study of shrinkage strains in a stereolithography cured acrylic photopolymer resin. J Mater Process Technol 2003, 136: 146–150.

    CAS  Article  Google Scholar 

  37. [37]

    Wang WL, Cheah CM, Fuh JYH, et al. Influence of process parameters on stereolithography part shrinkage. Mater Des 1996, 17: 205–213.

    CAS  Article  Google Scholar 

  38. [38]

    Xing HY, Zou B, Li SS, et al. Study on surface quality, precision and mechanical properties of 3D printed ZrO2 ceramic components by laser scanning stereolithography. Ceram Int 2017, 43: 16340–16347.

    CAS  Article  Google Scholar 

  39. [39]

    Schwentenwein M, Homa J. Additive manufacturing of dense alumina ceramics. Int J Appl Ceram Technol 2015, 12: 1–7.

    CAS  Article  Google Scholar 

  40. [40]

    Zhang KQ, He RJ, Ding GJ, et al. Digital light processing of 3Y-TZP strengthened ZrO2 ceramics. Mater Sci Eng: A 2020, 774: 138768.

    CAS  Article  Google Scholar 

  41. [41]

    Qu HW, Fu HY, Han ZY, et al. Biomaterials for bone tissue engineering scaffolds: A review. RSC Adv 2019, 9: 26252–26262.

    CAS  Article  Google Scholar 

  42. [42]

    Yang YW, Wang GY, Liang HX, et al. Additive manufacturing of bone scaffolds. Int J Bioprint 2019, 5: 148–172.

    CAS  Google Scholar 

  43. [43]

    Lin SJ, LeGeros RZ, Rohanizadeh R, et al. Biphasic calcium phosphate (BCP) bioceramics: Preparation and properties. Key Eng Mater 2003, 240-242: 473–476.

    CAS  Article  Google Scholar 

  44. [44]

    Zhao HX, Liang WH. A novel comby scaffold with improved mechanical strength for bone tissue engineering. Mater Lett 2017, 194: 220–223.

    CAS  Article  Google Scholar 

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This study is mainly financially supported by the Beijing Natural Science Foundation (2182064) hosted by Prof. Rujie He. Prof. Rujie He also thanks the support from the National Natural Science Foundation of China (51772028). Prof. Min Xia thanks the support from the Fundamental Research Funds for the Central Universities (3052017010). Prof. Xinxin Jin thanks the support from the National Natural Science Foundation of China (51602082). Dr. Keqiang Zhang thanks the support from the Graduate Technology Innovation Project of Beijing Institute of Technology (No. 2019CX10020).

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Correspondence to Rujie He or Chen Xie.

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Feng, C., Zhang, K., He, R. et al. Additive manufacturing of hydroxyapatite bioceramic scaffolds: Dispersion, digital light processing, sintering, mechanical properties, and biocompatibility. J Adv Ceram 9, 360–373 (2020).

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  • additive manufacturing
  • digital light processing
  • vat photopolymerization
  • hydroxyapatite
  • bioceramic scaffold